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Structural divergence of chromosomes between malaria vectors Anopheles lesteri and Anopheles sinensis
BACKGROUND: Anopheles lesteri and Anopheles sinensis are two major malaria vectors in China and Southeast Asia. They are dramatically different in terms of geographical distribution, host preference, resting habitats, and other traits associated with ecological adaptation and malaria transmission. B...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5124334/ https://www.ncbi.nlm.nih.gov/pubmed/27887641 http://dx.doi.org/10.1186/s13071-016-1855-0 |
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author | Liang, Jiangtao Cheng, Biao Zhu, Guoding Wei, Yun Tang, Jianxia Cao, Jun Ma, Yajun Sharakhova, Maria V. Xia, Ai Sharakhov, Igor V. |
author_facet | Liang, Jiangtao Cheng, Biao Zhu, Guoding Wei, Yun Tang, Jianxia Cao, Jun Ma, Yajun Sharakhova, Maria V. Xia, Ai Sharakhov, Igor V. |
author_sort | Liang, Jiangtao |
collection | PubMed |
description | BACKGROUND: Anopheles lesteri and Anopheles sinensis are two major malaria vectors in China and Southeast Asia. They are dramatically different in terms of geographical distribution, host preference, resting habitats, and other traits associated with ecological adaptation and malaria transmission. Both species belong to the Anopheles hyrcanus group, but the extent of genetic differences between them is not well understood. To provide an effective way to differentiate between species and to find useful markers for population genetics studies, we performed a comparative cytogenetic analysis of these two malaria vectors. RESULTS: Presented here is a standard cytogenetic map for An. lesteri, and a comparative analysis of chromosome structure and gene order between An. lesteri and An. sinensis. Our results demonstrate that much of the gene order on chromosomes X and 2 was reshuffled between the two species. However, the banding pattern and the gene order on chromosome 3 appeared to be conserved. We also found two new polymorphic inversions, 2Lc and 3Rb, in An. lesteri, and we mapped the breakpoints of these two inversions on polytene chromosomes. CONCLUSIONS: Our results demonstrate the extent of structural divergence of chromosomes between An. lesteri and An. sinensis, and provide a new taxonomic cytogenetic tool to distinguish between these two species. Polymorphic inversions of An. lesteri could serve as markers for studies of the population structure and ecological adaptations of this major malaria vector. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13071-016-1855-0) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5124334 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-51243342016-12-08 Structural divergence of chromosomes between malaria vectors Anopheles lesteri and Anopheles sinensis Liang, Jiangtao Cheng, Biao Zhu, Guoding Wei, Yun Tang, Jianxia Cao, Jun Ma, Yajun Sharakhova, Maria V. Xia, Ai Sharakhov, Igor V. Parasit Vectors Research BACKGROUND: Anopheles lesteri and Anopheles sinensis are two major malaria vectors in China and Southeast Asia. They are dramatically different in terms of geographical distribution, host preference, resting habitats, and other traits associated with ecological adaptation and malaria transmission. Both species belong to the Anopheles hyrcanus group, but the extent of genetic differences between them is not well understood. To provide an effective way to differentiate between species and to find useful markers for population genetics studies, we performed a comparative cytogenetic analysis of these two malaria vectors. RESULTS: Presented here is a standard cytogenetic map for An. lesteri, and a comparative analysis of chromosome structure and gene order between An. lesteri and An. sinensis. Our results demonstrate that much of the gene order on chromosomes X and 2 was reshuffled between the two species. However, the banding pattern and the gene order on chromosome 3 appeared to be conserved. We also found two new polymorphic inversions, 2Lc and 3Rb, in An. lesteri, and we mapped the breakpoints of these two inversions on polytene chromosomes. CONCLUSIONS: Our results demonstrate the extent of structural divergence of chromosomes between An. lesteri and An. sinensis, and provide a new taxonomic cytogenetic tool to distinguish between these two species. Polymorphic inversions of An. lesteri could serve as markers for studies of the population structure and ecological adaptations of this major malaria vector. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13071-016-1855-0) contains supplementary material, which is available to authorized users. BioMed Central 2016-11-25 /pmc/articles/PMC5124334/ /pubmed/27887641 http://dx.doi.org/10.1186/s13071-016-1855-0 Text en © The Author(s). 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Liang, Jiangtao Cheng, Biao Zhu, Guoding Wei, Yun Tang, Jianxia Cao, Jun Ma, Yajun Sharakhova, Maria V. Xia, Ai Sharakhov, Igor V. Structural divergence of chromosomes between malaria vectors Anopheles lesteri and Anopheles sinensis |
title | Structural divergence of chromosomes between malaria vectors Anopheles lesteri and Anopheles sinensis |
title_full | Structural divergence of chromosomes between malaria vectors Anopheles lesteri and Anopheles sinensis |
title_fullStr | Structural divergence of chromosomes between malaria vectors Anopheles lesteri and Anopheles sinensis |
title_full_unstemmed | Structural divergence of chromosomes between malaria vectors Anopheles lesteri and Anopheles sinensis |
title_short | Structural divergence of chromosomes between malaria vectors Anopheles lesteri and Anopheles sinensis |
title_sort | structural divergence of chromosomes between malaria vectors anopheles lesteri and anopheles sinensis |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5124334/ https://www.ncbi.nlm.nih.gov/pubmed/27887641 http://dx.doi.org/10.1186/s13071-016-1855-0 |
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