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Oxymatrine Inhibits Proliferation and Migration While Inducing Apoptosis in Human Glioblastoma Cells

Oxymatrine (OMT), an alkaloid derived from the traditional Chinese medicine herb Sophora flavescens Aiton, has been shown to exhibit anticancer properties on various types of cancer cells. In this study, we investigate the anticancer properties of OMT on human glioblastoma (GBM) cells and evaluate t...

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Autores principales: Liu, Feili, Wang, Baocheng, Wang, Jiajia, Ling, Xiaozheng, Li, Qifeng, Meng, Wei, Ma, Jie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5124477/
https://www.ncbi.nlm.nih.gov/pubmed/27957488
http://dx.doi.org/10.1155/2016/1784161
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author Liu, Feili
Wang, Baocheng
Wang, Jiajia
Ling, Xiaozheng
Li, Qifeng
Meng, Wei
Ma, Jie
author_facet Liu, Feili
Wang, Baocheng
Wang, Jiajia
Ling, Xiaozheng
Li, Qifeng
Meng, Wei
Ma, Jie
author_sort Liu, Feili
collection PubMed
description Oxymatrine (OMT), an alkaloid derived from the traditional Chinese medicine herb Sophora flavescens Aiton, has been shown to exhibit anticancer properties on various types of cancer cells. In this study, we investigate the anticancer properties of OMT on human glioblastoma (GBM) cells and evaluate their underlying mechanisms. MTT assays were performed and demonstrated that OMT significantly inhibits the proliferation of GBM cells. Flow cytometry suggested that OMT at a concentration of 10(−5) M may induce apoptosis in U251 and A172 cells. Western blot analyses demonstrated a significant increase in the expression of Bax and caspase-3 and a significant decrease in expression of Bcl-2 in both U251 and A172 cells. Additionally, OMT was found by transwell and high-content screening assays to decrease the migratory ability of the evaluated GBM cells. These findings suggest that the antitumor effects of OMT may be the result of inhibition of cell proliferation and migration and the induction of apoptosis by regulating the expression of apoptosis-associated proteins. OMT may represent a novel anticancer therapy for the treatment of GBM.
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spelling pubmed-51244772016-12-12 Oxymatrine Inhibits Proliferation and Migration While Inducing Apoptosis in Human Glioblastoma Cells Liu, Feili Wang, Baocheng Wang, Jiajia Ling, Xiaozheng Li, Qifeng Meng, Wei Ma, Jie Biomed Res Int Research Article Oxymatrine (OMT), an alkaloid derived from the traditional Chinese medicine herb Sophora flavescens Aiton, has been shown to exhibit anticancer properties on various types of cancer cells. In this study, we investigate the anticancer properties of OMT on human glioblastoma (GBM) cells and evaluate their underlying mechanisms. MTT assays were performed and demonstrated that OMT significantly inhibits the proliferation of GBM cells. Flow cytometry suggested that OMT at a concentration of 10(−5) M may induce apoptosis in U251 and A172 cells. Western blot analyses demonstrated a significant increase in the expression of Bax and caspase-3 and a significant decrease in expression of Bcl-2 in both U251 and A172 cells. Additionally, OMT was found by transwell and high-content screening assays to decrease the migratory ability of the evaluated GBM cells. These findings suggest that the antitumor effects of OMT may be the result of inhibition of cell proliferation and migration and the induction of apoptosis by regulating the expression of apoptosis-associated proteins. OMT may represent a novel anticancer therapy for the treatment of GBM. Hindawi Publishing Corporation 2016 2016-11-13 /pmc/articles/PMC5124477/ /pubmed/27957488 http://dx.doi.org/10.1155/2016/1784161 Text en Copyright © 2016 Feili Liu et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Liu, Feili
Wang, Baocheng
Wang, Jiajia
Ling, Xiaozheng
Li, Qifeng
Meng, Wei
Ma, Jie
Oxymatrine Inhibits Proliferation and Migration While Inducing Apoptosis in Human Glioblastoma Cells
title Oxymatrine Inhibits Proliferation and Migration While Inducing Apoptosis in Human Glioblastoma Cells
title_full Oxymatrine Inhibits Proliferation and Migration While Inducing Apoptosis in Human Glioblastoma Cells
title_fullStr Oxymatrine Inhibits Proliferation and Migration While Inducing Apoptosis in Human Glioblastoma Cells
title_full_unstemmed Oxymatrine Inhibits Proliferation and Migration While Inducing Apoptosis in Human Glioblastoma Cells
title_short Oxymatrine Inhibits Proliferation and Migration While Inducing Apoptosis in Human Glioblastoma Cells
title_sort oxymatrine inhibits proliferation and migration while inducing apoptosis in human glioblastoma cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5124477/
https://www.ncbi.nlm.nih.gov/pubmed/27957488
http://dx.doi.org/10.1155/2016/1784161
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