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Metabolic engineering of Serratia marcescens MG1 for enhanced production of (3R)-acetoin
BACKGROUND: Optically pure acetoin (AC) is an important platform chemical which has been widely used to synthesize novel optically active α-hydroxyketone derivatives and liquid crystal composites. RESULTS: In this study, slaC and gldA encoding meso-2,3-butanediol dehydrogenase (meso-2,3-BDH) and gly...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5124605/ https://www.ncbi.nlm.nih.gov/pubmed/27942437 http://dx.doi.org/10.1186/s40643-016-0128-2 |
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author | Lv, Xin Dai, Lu Bai, Fangmin Wang, Zhanqing Zhang, Liaoyuan Shen, Yaling |
author_facet | Lv, Xin Dai, Lu Bai, Fangmin Wang, Zhanqing Zhang, Liaoyuan Shen, Yaling |
author_sort | Lv, Xin |
collection | PubMed |
description | BACKGROUND: Optically pure acetoin (AC) is an important platform chemical which has been widely used to synthesize novel optically active α-hydroxyketone derivatives and liquid crystal composites. RESULTS: In this study, slaC and gldA encoding meso-2,3-butanediol dehydrogenase (meso-2,3-BDH) and glycerol dehydrogenase (GDH), respectively, in S. marcescens MG1 were knocked out to block the conversion from AC to 2,3-butanediol (2,3-BD). The resulting strain MG14 was found to produce a large amount of optically pure (3R)-AC with a little 2,3-BD, indicating that another enzyme responsible for 2,3-BD formation except meso-2,3-BDH and GDH existed in the strain MG1. Furthermore, SlaR protein, a transcriptional activator of AC cluster, was overexpressed using P (C) promoter in the strain MG14, leading to enhancement of the (3R)-AC yield by 29.91%. The recombinant strain with overexpression of SlaR, designated as S. marcescens MG15, was used to perform medium optimization for improving (3R)-AC production. CONCLUSION: Under the optimized conditions, 39.91 ± 1.35 g/l (3R)-AC was produced by strain MG15 with the productivity of 1.11 g/l h and the conversion rate of 80.13%. |
format | Online Article Text |
id | pubmed-5124605 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-51246052016-12-09 Metabolic engineering of Serratia marcescens MG1 for enhanced production of (3R)-acetoin Lv, Xin Dai, Lu Bai, Fangmin Wang, Zhanqing Zhang, Liaoyuan Shen, Yaling Bioresour Bioprocess Research BACKGROUND: Optically pure acetoin (AC) is an important platform chemical which has been widely used to synthesize novel optically active α-hydroxyketone derivatives and liquid crystal composites. RESULTS: In this study, slaC and gldA encoding meso-2,3-butanediol dehydrogenase (meso-2,3-BDH) and glycerol dehydrogenase (GDH), respectively, in S. marcescens MG1 were knocked out to block the conversion from AC to 2,3-butanediol (2,3-BD). The resulting strain MG14 was found to produce a large amount of optically pure (3R)-AC with a little 2,3-BD, indicating that another enzyme responsible for 2,3-BD formation except meso-2,3-BDH and GDH existed in the strain MG1. Furthermore, SlaR protein, a transcriptional activator of AC cluster, was overexpressed using P (C) promoter in the strain MG14, leading to enhancement of the (3R)-AC yield by 29.91%. The recombinant strain with overexpression of SlaR, designated as S. marcescens MG15, was used to perform medium optimization for improving (3R)-AC production. CONCLUSION: Under the optimized conditions, 39.91 ± 1.35 g/l (3R)-AC was produced by strain MG15 with the productivity of 1.11 g/l h and the conversion rate of 80.13%. Springer Berlin Heidelberg 2016-11-28 2016 /pmc/articles/PMC5124605/ /pubmed/27942437 http://dx.doi.org/10.1186/s40643-016-0128-2 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Research Lv, Xin Dai, Lu Bai, Fangmin Wang, Zhanqing Zhang, Liaoyuan Shen, Yaling Metabolic engineering of Serratia marcescens MG1 for enhanced production of (3R)-acetoin |
title | Metabolic engineering of Serratia marcescens MG1 for enhanced production of (3R)-acetoin |
title_full | Metabolic engineering of Serratia marcescens MG1 for enhanced production of (3R)-acetoin |
title_fullStr | Metabolic engineering of Serratia marcescens MG1 for enhanced production of (3R)-acetoin |
title_full_unstemmed | Metabolic engineering of Serratia marcescens MG1 for enhanced production of (3R)-acetoin |
title_short | Metabolic engineering of Serratia marcescens MG1 for enhanced production of (3R)-acetoin |
title_sort | metabolic engineering of serratia marcescens mg1 for enhanced production of (3r)-acetoin |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5124605/ https://www.ncbi.nlm.nih.gov/pubmed/27942437 http://dx.doi.org/10.1186/s40643-016-0128-2 |
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