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Clinical Evaluation of Humira(®) Biosimilar ONS-3010 in Healthy Volunteers: Focus on Pharmacokinetics and Pharmacodynamics

ONS-3010 is being developed by Oncobiologics Inc. (Cranbury, NJ, USA) as a biosimilar of Humira(®). This randomized, double blind, single-center phase I study (EudraCT registration # 2013-003551-38) was performed to demonstrate pharmacokinetic (PK) biosimilarity between two reference products (Humir...

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Autores principales: Dillingh, Marlous R., Reijers, Joannes A. A., Malone, Karen E., Burggraaf, Jacobus, Bahrt, Kenneth, Yamashita, Liz, Rehrig, Claudia, Moerland, Matthijs
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5124695/
https://www.ncbi.nlm.nih.gov/pubmed/27965661
http://dx.doi.org/10.3389/fimmu.2016.00508
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author Dillingh, Marlous R.
Reijers, Joannes A. A.
Malone, Karen E.
Burggraaf, Jacobus
Bahrt, Kenneth
Yamashita, Liz
Rehrig, Claudia
Moerland, Matthijs
author_facet Dillingh, Marlous R.
Reijers, Joannes A. A.
Malone, Karen E.
Burggraaf, Jacobus
Bahrt, Kenneth
Yamashita, Liz
Rehrig, Claudia
Moerland, Matthijs
author_sort Dillingh, Marlous R.
collection PubMed
description ONS-3010 is being developed by Oncobiologics Inc. (Cranbury, NJ, USA) as a biosimilar of Humira(®). This randomized, double blind, single-center phase I study (EudraCT registration # 2013-003551-38) was performed to demonstrate pharmacokinetic (PK) biosimilarity between two reference products (Humira(®) EU and US) and ONS-3010 in healthy volunteers, and to compare the safety and immunogenicity profiles. In addition, the intended pharmacological activity was assessed and compared by application of a whole blood challenge. Hundred ninety-eight healthy volunteers received a single 40 mg subcutaneous dose of ONS-3010, Humira(®) EU, or US. The pharmacodynamic effects were assessed by lipopolysaccharide (LPS)/aluminum hydroxide whole blood challenges (n = 36; n = 12 per treatment arm; male:female, 1:1). Equivalence was demonstrated on the PK endpoints (AUC(0–inf), C(max), and AUC(0–last)) based on bounds of 80–125% for the ratio of the geometric means (ONS-3010/Humira(®)). The immunogenicity profiles were comparable between treatment groups, and there were no indications for differences in routine safety parameters. Administration of adalimumab resulted in the observation of dramatically reduced tumor necrosis factor-α (TNFα) levels upon stimulation with LPS/aluminum hydroxide (>99%), with no differences between the three treatment groups in terms of magnitude or duration. Adalimumab also resulted in a reduction of LPS/aluminum hydroxide-induced interleukin (IL)-8 release (maximally 30%), suggested to have a causal relationship with the anti-TNFα treatment. LPS/aluminum hydroxide-induced release of IL-1β and IL-6 was not inhibited by anti-TNFα treatment. Taken together, these data are promising for the further clinical development of ONS-3010, demonstrate the relevance of the LPS/aluminum challenge to monitor Humira(®) effects, and emphasize the value of whole blood challenges for monitoring of proximal drug effects in healthy volunteers, and potentially in the target population.
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spelling pubmed-51246952016-12-13 Clinical Evaluation of Humira(®) Biosimilar ONS-3010 in Healthy Volunteers: Focus on Pharmacokinetics and Pharmacodynamics Dillingh, Marlous R. Reijers, Joannes A. A. Malone, Karen E. Burggraaf, Jacobus Bahrt, Kenneth Yamashita, Liz Rehrig, Claudia Moerland, Matthijs Front Immunol Immunology ONS-3010 is being developed by Oncobiologics Inc. (Cranbury, NJ, USA) as a biosimilar of Humira(®). This randomized, double blind, single-center phase I study (EudraCT registration # 2013-003551-38) was performed to demonstrate pharmacokinetic (PK) biosimilarity between two reference products (Humira(®) EU and US) and ONS-3010 in healthy volunteers, and to compare the safety and immunogenicity profiles. In addition, the intended pharmacological activity was assessed and compared by application of a whole blood challenge. Hundred ninety-eight healthy volunteers received a single 40 mg subcutaneous dose of ONS-3010, Humira(®) EU, or US. The pharmacodynamic effects were assessed by lipopolysaccharide (LPS)/aluminum hydroxide whole blood challenges (n = 36; n = 12 per treatment arm; male:female, 1:1). Equivalence was demonstrated on the PK endpoints (AUC(0–inf), C(max), and AUC(0–last)) based on bounds of 80–125% for the ratio of the geometric means (ONS-3010/Humira(®)). The immunogenicity profiles were comparable between treatment groups, and there were no indications for differences in routine safety parameters. Administration of adalimumab resulted in the observation of dramatically reduced tumor necrosis factor-α (TNFα) levels upon stimulation with LPS/aluminum hydroxide (>99%), with no differences between the three treatment groups in terms of magnitude or duration. Adalimumab also resulted in a reduction of LPS/aluminum hydroxide-induced interleukin (IL)-8 release (maximally 30%), suggested to have a causal relationship with the anti-TNFα treatment. LPS/aluminum hydroxide-induced release of IL-1β and IL-6 was not inhibited by anti-TNFα treatment. Taken together, these data are promising for the further clinical development of ONS-3010, demonstrate the relevance of the LPS/aluminum challenge to monitor Humira(®) effects, and emphasize the value of whole blood challenges for monitoring of proximal drug effects in healthy volunteers, and potentially in the target population. Frontiers Media S.A. 2016-11-28 /pmc/articles/PMC5124695/ /pubmed/27965661 http://dx.doi.org/10.3389/fimmu.2016.00508 Text en Copyright © 2016 Dillingh, Reijers, Malone, Burggraaf, Bahrt, Yamashita, Rehrig and Moerland. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Dillingh, Marlous R.
Reijers, Joannes A. A.
Malone, Karen E.
Burggraaf, Jacobus
Bahrt, Kenneth
Yamashita, Liz
Rehrig, Claudia
Moerland, Matthijs
Clinical Evaluation of Humira(®) Biosimilar ONS-3010 in Healthy Volunteers: Focus on Pharmacokinetics and Pharmacodynamics
title Clinical Evaluation of Humira(®) Biosimilar ONS-3010 in Healthy Volunteers: Focus on Pharmacokinetics and Pharmacodynamics
title_full Clinical Evaluation of Humira(®) Biosimilar ONS-3010 in Healthy Volunteers: Focus on Pharmacokinetics and Pharmacodynamics
title_fullStr Clinical Evaluation of Humira(®) Biosimilar ONS-3010 in Healthy Volunteers: Focus on Pharmacokinetics and Pharmacodynamics
title_full_unstemmed Clinical Evaluation of Humira(®) Biosimilar ONS-3010 in Healthy Volunteers: Focus on Pharmacokinetics and Pharmacodynamics
title_short Clinical Evaluation of Humira(®) Biosimilar ONS-3010 in Healthy Volunteers: Focus on Pharmacokinetics and Pharmacodynamics
title_sort clinical evaluation of humira(®) biosimilar ons-3010 in healthy volunteers: focus on pharmacokinetics and pharmacodynamics
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5124695/
https://www.ncbi.nlm.nih.gov/pubmed/27965661
http://dx.doi.org/10.3389/fimmu.2016.00508
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