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Effects of ω-3 fatty acids on toll-like receptor 4 and nuclear factor-κB p56 in lungs of rats with severe acute pancreatitis

AIM: To determine the effects of ω-3 fatty acids (ω-3FA) on the toll-like receptor 4 (TLR4)/nuclear factor κB p56 (NF-κBp56) signal pathway in the lungs of rats with severe acute pancreatitis (SAP). METHODS: A total of 56 Sprague-Dawley rats were randomly divided into 4 groups: control group, SAP-sa...

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Autores principales: Wang, Bin, Wu, Xiao-Wei, Guo, Mei-Xia, Li, Min-Li, Xu, Xiao-Bing, Jin, Xin-Xin, Zhang, Xiao-Hua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Baishideng Publishing Group Inc 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5124983/
https://www.ncbi.nlm.nih.gov/pubmed/27956802
http://dx.doi.org/10.3748/wjg.v22.i44.9784
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author Wang, Bin
Wu, Xiao-Wei
Guo, Mei-Xia
Li, Min-Li
Xu, Xiao-Bing
Jin, Xin-Xin
Zhang, Xiao-Hua
author_facet Wang, Bin
Wu, Xiao-Wei
Guo, Mei-Xia
Li, Min-Li
Xu, Xiao-Bing
Jin, Xin-Xin
Zhang, Xiao-Hua
author_sort Wang, Bin
collection PubMed
description AIM: To determine the effects of ω-3 fatty acids (ω-3FA) on the toll-like receptor 4 (TLR4)/nuclear factor κB p56 (NF-κBp56) signal pathway in the lungs of rats with severe acute pancreatitis (SAP). METHODS: A total of 56 Sprague-Dawley rats were randomly divided into 4 groups: control group, SAP-saline group, SAP-soybean oil group and SAP-ω-3FA group. SAP was induced by the retrograde infusion of sodium taurocholate into the pancreatic duct. The expression of TLR4 and NF-κBp56 in the lungs was evaluated by immunohistochemistry and Western blot analysis. The levels of inflammatory cytokines interleukin-6 and tumor necrosis factor-alpha in the lungs were measured by enzyme-linked immunosorbent assay. RESULTS: The expression of TLR4 and NF-κBp56 in lungs and of inflammatory cytokines in serum significantly increased in the SAP group compared with the control group (P < 0.05), but was significantly decreased in the ω-3FA group compared with the soybean oil group at 12 and 24 h (P < 0.05). CONCLUSION: During the initial stage of SAP, ω-3FA can efficiently lower the inflammatory response and reduce lung injury by triggering the TLR4/NF-κBp56 signal pathway.
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spelling pubmed-51249832016-12-12 Effects of ω-3 fatty acids on toll-like receptor 4 and nuclear factor-κB p56 in lungs of rats with severe acute pancreatitis Wang, Bin Wu, Xiao-Wei Guo, Mei-Xia Li, Min-Li Xu, Xiao-Bing Jin, Xin-Xin Zhang, Xiao-Hua World J Gastroenterol Basic Study AIM: To determine the effects of ω-3 fatty acids (ω-3FA) on the toll-like receptor 4 (TLR4)/nuclear factor κB p56 (NF-κBp56) signal pathway in the lungs of rats with severe acute pancreatitis (SAP). METHODS: A total of 56 Sprague-Dawley rats were randomly divided into 4 groups: control group, SAP-saline group, SAP-soybean oil group and SAP-ω-3FA group. SAP was induced by the retrograde infusion of sodium taurocholate into the pancreatic duct. The expression of TLR4 and NF-κBp56 in the lungs was evaluated by immunohistochemistry and Western blot analysis. The levels of inflammatory cytokines interleukin-6 and tumor necrosis factor-alpha in the lungs were measured by enzyme-linked immunosorbent assay. RESULTS: The expression of TLR4 and NF-κBp56 in lungs and of inflammatory cytokines in serum significantly increased in the SAP group compared with the control group (P < 0.05), but was significantly decreased in the ω-3FA group compared with the soybean oil group at 12 and 24 h (P < 0.05). CONCLUSION: During the initial stage of SAP, ω-3FA can efficiently lower the inflammatory response and reduce lung injury by triggering the TLR4/NF-κBp56 signal pathway. Baishideng Publishing Group Inc 2016-11-28 2016-11-28 /pmc/articles/PMC5124983/ /pubmed/27956802 http://dx.doi.org/10.3748/wjg.v22.i44.9784 Text en ©The Author(s) 2016. Published by Baishideng Publishing Group Inc. All rights reserved. http://creativecommons.org/licenses/by-nc/4.0/ This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial.
spellingShingle Basic Study
Wang, Bin
Wu, Xiao-Wei
Guo, Mei-Xia
Li, Min-Li
Xu, Xiao-Bing
Jin, Xin-Xin
Zhang, Xiao-Hua
Effects of ω-3 fatty acids on toll-like receptor 4 and nuclear factor-κB p56 in lungs of rats with severe acute pancreatitis
title Effects of ω-3 fatty acids on toll-like receptor 4 and nuclear factor-κB p56 in lungs of rats with severe acute pancreatitis
title_full Effects of ω-3 fatty acids on toll-like receptor 4 and nuclear factor-κB p56 in lungs of rats with severe acute pancreatitis
title_fullStr Effects of ω-3 fatty acids on toll-like receptor 4 and nuclear factor-κB p56 in lungs of rats with severe acute pancreatitis
title_full_unstemmed Effects of ω-3 fatty acids on toll-like receptor 4 and nuclear factor-κB p56 in lungs of rats with severe acute pancreatitis
title_short Effects of ω-3 fatty acids on toll-like receptor 4 and nuclear factor-κB p56 in lungs of rats with severe acute pancreatitis
title_sort effects of ω-3 fatty acids on toll-like receptor 4 and nuclear factor-κb p56 in lungs of rats with severe acute pancreatitis
topic Basic Study
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5124983/
https://www.ncbi.nlm.nih.gov/pubmed/27956802
http://dx.doi.org/10.3748/wjg.v22.i44.9784
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