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Disease-specific miR-34a as diagnostic marker of non-alcoholic steatohepatitis in a Chinese population

AIM: To assess disease-specific circulating microRNAs (miRNAs) in non-alcoholic steatohepatitis (NASH) patients. METHODS: A total of 111 biopsy-proven non-alcoholic fatty liver disease (NAFLD) or chronic hepatitis B (CHB) patients and healthy controls from mainland China were enrolled to measure the...

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Autores principales: Liu, Xiao-Lin, Pan, Qin, Zhang, Rui-Nan, Shen, Feng, Yan, Shi-Yan, Sun, Chao, Xu, Zheng-Jie, Chen, Yuan-Wen, Fan, Jian-Gao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Baishideng Publishing Group Inc 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5124990/
https://www.ncbi.nlm.nih.gov/pubmed/27956809
http://dx.doi.org/10.3748/wjg.v22.i44.9844
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author Liu, Xiao-Lin
Pan, Qin
Zhang, Rui-Nan
Shen, Feng
Yan, Shi-Yan
Sun, Chao
Xu, Zheng-Jie
Chen, Yuan-Wen
Fan, Jian-Gao
author_facet Liu, Xiao-Lin
Pan, Qin
Zhang, Rui-Nan
Shen, Feng
Yan, Shi-Yan
Sun, Chao
Xu, Zheng-Jie
Chen, Yuan-Wen
Fan, Jian-Gao
author_sort Liu, Xiao-Lin
collection PubMed
description AIM: To assess disease-specific circulating microRNAs (miRNAs) in non-alcoholic steatohepatitis (NASH) patients. METHODS: A total of 111 biopsy-proven non-alcoholic fatty liver disease (NAFLD) or chronic hepatitis B (CHB) patients and healthy controls from mainland China were enrolled to measure their serum levels of miR-122, -125b, -146b, -16, -21, -192, -27b and -34a. The correlations between serum miRNAs and histological features of NAFLD were determined. The diagnostic value of miRNA in NASH and significant fibrosis was analyzed and compared with that of cytokeratin-18 (CK-18), fibrosis-4 (FIB-4), and aspartate aminotransferase to platelet ratio index (APRI), respectively. RESULTS: Circulating miR-122, -16, -192 and -34a showed differential expression levels between NAFLD and CHB patients, and miR-34a had an approximately 2-fold increase in NAFLD samples compared with that of CHB samples (P < 0.01). Serum miR-122, -192 and -34a levels were correlated with steatosis (R = 0.302, 0.323 and 0.470, respectively, P < 0.05) and inflammatory activity (R = 0.445, 0.447 and 0.517, respectively, P < 0.01); only serum miR-16 levels were associated with fibrosis (R = 0.350, P < 0.05) in patients with NAFLD. The diagnostic value of miR-34a for NASH (area under the receiver operating characteristic, 0.811, 95%CI: 0.670-0.953) was superior to that of alanine aminotransferase, CK-18, FIB-4 and APRI in NAFLD, but miR-16 showed a limited performance in the diagnosis of significant fibrosis in NASH. CONCLUSION: Circulating miR-34a may serve as a disease-specific noninvasive biomarker for the diagnosis of NASH.
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spelling pubmed-51249902016-12-12 Disease-specific miR-34a as diagnostic marker of non-alcoholic steatohepatitis in a Chinese population Liu, Xiao-Lin Pan, Qin Zhang, Rui-Nan Shen, Feng Yan, Shi-Yan Sun, Chao Xu, Zheng-Jie Chen, Yuan-Wen Fan, Jian-Gao World J Gastroenterol Observational Study AIM: To assess disease-specific circulating microRNAs (miRNAs) in non-alcoholic steatohepatitis (NASH) patients. METHODS: A total of 111 biopsy-proven non-alcoholic fatty liver disease (NAFLD) or chronic hepatitis B (CHB) patients and healthy controls from mainland China were enrolled to measure their serum levels of miR-122, -125b, -146b, -16, -21, -192, -27b and -34a. The correlations between serum miRNAs and histological features of NAFLD were determined. The diagnostic value of miRNA in NASH and significant fibrosis was analyzed and compared with that of cytokeratin-18 (CK-18), fibrosis-4 (FIB-4), and aspartate aminotransferase to platelet ratio index (APRI), respectively. RESULTS: Circulating miR-122, -16, -192 and -34a showed differential expression levels between NAFLD and CHB patients, and miR-34a had an approximately 2-fold increase in NAFLD samples compared with that of CHB samples (P < 0.01). Serum miR-122, -192 and -34a levels were correlated with steatosis (R = 0.302, 0.323 and 0.470, respectively, P < 0.05) and inflammatory activity (R = 0.445, 0.447 and 0.517, respectively, P < 0.01); only serum miR-16 levels were associated with fibrosis (R = 0.350, P < 0.05) in patients with NAFLD. The diagnostic value of miR-34a for NASH (area under the receiver operating characteristic, 0.811, 95%CI: 0.670-0.953) was superior to that of alanine aminotransferase, CK-18, FIB-4 and APRI in NAFLD, but miR-16 showed a limited performance in the diagnosis of significant fibrosis in NASH. CONCLUSION: Circulating miR-34a may serve as a disease-specific noninvasive biomarker for the diagnosis of NASH. Baishideng Publishing Group Inc 2016-11-28 2016-11-28 /pmc/articles/PMC5124990/ /pubmed/27956809 http://dx.doi.org/10.3748/wjg.v22.i44.9844 Text en ©The Author(s) 2016. Published by Baishideng Publishing Group Inc. All rights reserved. http://creativecommons.org/licenses/by-nc/4.0/ This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial.
spellingShingle Observational Study
Liu, Xiao-Lin
Pan, Qin
Zhang, Rui-Nan
Shen, Feng
Yan, Shi-Yan
Sun, Chao
Xu, Zheng-Jie
Chen, Yuan-Wen
Fan, Jian-Gao
Disease-specific miR-34a as diagnostic marker of non-alcoholic steatohepatitis in a Chinese population
title Disease-specific miR-34a as diagnostic marker of non-alcoholic steatohepatitis in a Chinese population
title_full Disease-specific miR-34a as diagnostic marker of non-alcoholic steatohepatitis in a Chinese population
title_fullStr Disease-specific miR-34a as diagnostic marker of non-alcoholic steatohepatitis in a Chinese population
title_full_unstemmed Disease-specific miR-34a as diagnostic marker of non-alcoholic steatohepatitis in a Chinese population
title_short Disease-specific miR-34a as diagnostic marker of non-alcoholic steatohepatitis in a Chinese population
title_sort disease-specific mir-34a as diagnostic marker of non-alcoholic steatohepatitis in a chinese population
topic Observational Study
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5124990/
https://www.ncbi.nlm.nih.gov/pubmed/27956809
http://dx.doi.org/10.3748/wjg.v22.i44.9844
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