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Recognition of extremophilic archaeal viruses by eukaryotic cells: a promising nanoplatform from the third domain of life

Viruses from the third domain of life, Archaea, exhibit unusual features including extreme stability that allow their survival in harsh environments. In addition, these species have never been reported to integrate into human or any other eukaryotic genomes, and could thus serve for exploration of n...

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Autores principales: Uldahl, Kristine Buch, Wu, Linping, Hall, Arnaldur, Papathanasiou, Pavlos, Peng, Xu, Moghimi, Seyed Moein
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5125014/
https://www.ncbi.nlm.nih.gov/pubmed/27892499
http://dx.doi.org/10.1038/srep37966
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author Uldahl, Kristine Buch
Wu, Linping
Hall, Arnaldur
Papathanasiou, Pavlos
Peng, Xu
Moghimi, Seyed Moein
author_facet Uldahl, Kristine Buch
Wu, Linping
Hall, Arnaldur
Papathanasiou, Pavlos
Peng, Xu
Moghimi, Seyed Moein
author_sort Uldahl, Kristine Buch
collection PubMed
description Viruses from the third domain of life, Archaea, exhibit unusual features including extreme stability that allow their survival in harsh environments. In addition, these species have never been reported to integrate into human or any other eukaryotic genomes, and could thus serve for exploration of novel medical nanoplatforms. Here, we selected two archaeal viruses Sulfolobus monocaudavirus 1 (SMV1) and Sulfolobus spindle shaped virus 2 (SSV2) owing to their unique spindle shape, hyperthermostable and acid-resistant nature and studied their interaction with mammalian cells. Accordingly, we followed viral uptake, intracellular trafficking and cell viability in human endothelial cells of brain (hCMEC/D3 cells) and umbilical vein (HUVEC) origin. Whereas SMV1 is efficiently internalized into both types of human cells, SSV2 differentiates between HUVECs and hCMEC/D3 cells, thus opening a path for selective cell targeting. On internalization, both viruses localize to the lysosomal compartments. Neither SMV1, nor SSV2 induced any detrimental effect on cell morphology, plasma membrane and mitochondrial functionality. This is the first study demonstrating recognition of archaeal viruses by eukaryotic cells which provides good basis for future exploration of archaeal viruses in bioengineering and development of multifunctional vectors.
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spelling pubmed-51250142016-12-08 Recognition of extremophilic archaeal viruses by eukaryotic cells: a promising nanoplatform from the third domain of life Uldahl, Kristine Buch Wu, Linping Hall, Arnaldur Papathanasiou, Pavlos Peng, Xu Moghimi, Seyed Moein Sci Rep Article Viruses from the third domain of life, Archaea, exhibit unusual features including extreme stability that allow their survival in harsh environments. In addition, these species have never been reported to integrate into human or any other eukaryotic genomes, and could thus serve for exploration of novel medical nanoplatforms. Here, we selected two archaeal viruses Sulfolobus monocaudavirus 1 (SMV1) and Sulfolobus spindle shaped virus 2 (SSV2) owing to their unique spindle shape, hyperthermostable and acid-resistant nature and studied their interaction with mammalian cells. Accordingly, we followed viral uptake, intracellular trafficking and cell viability in human endothelial cells of brain (hCMEC/D3 cells) and umbilical vein (HUVEC) origin. Whereas SMV1 is efficiently internalized into both types of human cells, SSV2 differentiates between HUVECs and hCMEC/D3 cells, thus opening a path for selective cell targeting. On internalization, both viruses localize to the lysosomal compartments. Neither SMV1, nor SSV2 induced any detrimental effect on cell morphology, plasma membrane and mitochondrial functionality. This is the first study demonstrating recognition of archaeal viruses by eukaryotic cells which provides good basis for future exploration of archaeal viruses in bioengineering and development of multifunctional vectors. Nature Publishing Group 2016-11-28 /pmc/articles/PMC5125014/ /pubmed/27892499 http://dx.doi.org/10.1038/srep37966 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Uldahl, Kristine Buch
Wu, Linping
Hall, Arnaldur
Papathanasiou, Pavlos
Peng, Xu
Moghimi, Seyed Moein
Recognition of extremophilic archaeal viruses by eukaryotic cells: a promising nanoplatform from the third domain of life
title Recognition of extremophilic archaeal viruses by eukaryotic cells: a promising nanoplatform from the third domain of life
title_full Recognition of extremophilic archaeal viruses by eukaryotic cells: a promising nanoplatform from the third domain of life
title_fullStr Recognition of extremophilic archaeal viruses by eukaryotic cells: a promising nanoplatform from the third domain of life
title_full_unstemmed Recognition of extremophilic archaeal viruses by eukaryotic cells: a promising nanoplatform from the third domain of life
title_short Recognition of extremophilic archaeal viruses by eukaryotic cells: a promising nanoplatform from the third domain of life
title_sort recognition of extremophilic archaeal viruses by eukaryotic cells: a promising nanoplatform from the third domain of life
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5125014/
https://www.ncbi.nlm.nih.gov/pubmed/27892499
http://dx.doi.org/10.1038/srep37966
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