Identification of differentially methylated regions (DMRs) of neuronatin in mice

BACKGROUND: Neuronatin (NNAT) is a paternal-inherited imprinted gene, first discovered in the rat neonatal brain, where it plays vital roles for neuronal growth, brain development, and metabolic regulation. The maternal imprint of NNAT has been identified in mice; however, the differentially methyla...

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Autores principales: Xu, Yuxin, Liu, Zhiquan, Wang, Tiedong, Chen, Xianju, Deng, Jichao, Chen, Mao, Li, Zhanjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2016
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5125312/
https://www.ncbi.nlm.nih.gov/pubmed/27994995
http://dx.doi.org/10.1186/s40064-016-3721-0
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author Xu, Yuxin
Liu, Zhiquan
Wang, Tiedong
Chen, Xianju
Deng, Jichao
Chen, Mao
Li, Zhanjun
author_facet Xu, Yuxin
Liu, Zhiquan
Wang, Tiedong
Chen, Xianju
Deng, Jichao
Chen, Mao
Li, Zhanjun
author_sort Xu, Yuxin
collection PubMed
description BACKGROUND: Neuronatin (NNAT) is a paternal-inherited imprinted gene, first discovered in the rat neonatal brain, where it plays vital roles for neuronal growth, brain development, and metabolic regulation. The maternal imprint of NNAT has been identified in mice; however, the differentially methylated regions (DMRs) involved in the monoallelic expression of NNAT have not yet been investigated. RESULTS: In this study, we confirmed expression of two isoforms of the NNAT (α and β) in the mice brain via quantitative RT-PCR. Additionally, the methylation profile of the CpG island located in the NNAT gene locus was determined in the mice liver, brain, sperm, and the MII oocyte via bisulfite sequencing PCR. CONCLUSION: In summary, we provide the first evidence for tissue- and gamete-specific methylation patterns of CpG3 that are located on exon 1, to be putative DMR of NNAT in mice.
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spelling pubmed-51253122016-12-19 Identification of differentially methylated regions (DMRs) of neuronatin in mice Xu, Yuxin Liu, Zhiquan Wang, Tiedong Chen, Xianju Deng, Jichao Chen, Mao Li, Zhanjun Springerplus Research BACKGROUND: Neuronatin (NNAT) is a paternal-inherited imprinted gene, first discovered in the rat neonatal brain, where it plays vital roles for neuronal growth, brain development, and metabolic regulation. The maternal imprint of NNAT has been identified in mice; however, the differentially methylated regions (DMRs) involved in the monoallelic expression of NNAT have not yet been investigated. RESULTS: In this study, we confirmed expression of two isoforms of the NNAT (α and β) in the mice brain via quantitative RT-PCR. Additionally, the methylation profile of the CpG island located in the NNAT gene locus was determined in the mice liver, brain, sperm, and the MII oocyte via bisulfite sequencing PCR. CONCLUSION: In summary, we provide the first evidence for tissue- and gamete-specific methylation patterns of CpG3 that are located on exon 1, to be putative DMR of NNAT in mice. Springer International Publishing 2016-11-28 /pmc/articles/PMC5125312/ /pubmed/27994995 http://dx.doi.org/10.1186/s40064-016-3721-0 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Research
Xu, Yuxin
Liu, Zhiquan
Wang, Tiedong
Chen, Xianju
Deng, Jichao
Chen, Mao
Li, Zhanjun
Identification of differentially methylated regions (DMRs) of neuronatin in mice
title Identification of differentially methylated regions (DMRs) of neuronatin in mice
title_full Identification of differentially methylated regions (DMRs) of neuronatin in mice
title_fullStr Identification of differentially methylated regions (DMRs) of neuronatin in mice
title_full_unstemmed Identification of differentially methylated regions (DMRs) of neuronatin in mice
title_short Identification of differentially methylated regions (DMRs) of neuronatin in mice
title_sort identification of differentially methylated regions (dmrs) of neuronatin in mice
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5125312/
https://www.ncbi.nlm.nih.gov/pubmed/27994995
http://dx.doi.org/10.1186/s40064-016-3721-0
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