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Differential Protein Expression in the Hemolymph of Bithynia siamensis goniomphalos Infected with Opisthorchis viverrini

Bithynia siamensis goniomphalos is a freshwater snail that serves as the first intermediate host of the human liver fluke Opisthorchis viverrini. This parasite is a major public health problem in different countries throughout the Greater Mekong sub-region (Thailand, southern Vietnam, Lao PDR and Ca...

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Autores principales: Suwannatrai, Kulwadee, Suwannatrai, Apiporn, Tabsripair, Pairat, Welbat, Jariya Umka, Tangkawattana, Sirikachorn, Cantacessi, Cinzia, Mulvenna, Jason, Tesana, Smarn, Loukas, Alex, Sotillo, Javier
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5125571/
https://www.ncbi.nlm.nih.gov/pubmed/27893749
http://dx.doi.org/10.1371/journal.pntd.0005104
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author Suwannatrai, Kulwadee
Suwannatrai, Apiporn
Tabsripair, Pairat
Welbat, Jariya Umka
Tangkawattana, Sirikachorn
Cantacessi, Cinzia
Mulvenna, Jason
Tesana, Smarn
Loukas, Alex
Sotillo, Javier
author_facet Suwannatrai, Kulwadee
Suwannatrai, Apiporn
Tabsripair, Pairat
Welbat, Jariya Umka
Tangkawattana, Sirikachorn
Cantacessi, Cinzia
Mulvenna, Jason
Tesana, Smarn
Loukas, Alex
Sotillo, Javier
author_sort Suwannatrai, Kulwadee
collection PubMed
description Bithynia siamensis goniomphalos is a freshwater snail that serves as the first intermediate host of the human liver fluke Opisthorchis viverrini. This parasite is a major public health problem in different countries throughout the Greater Mekong sub-region (Thailand, southern Vietnam, Lao PDR and Cambodia). Chronic O. viverrini infection also results in a gradual increase of fibrotic tissues in the biliary tract that are associated with hepatobiliary diseases and contribute to cholangiocarcinoma (a fatal type of bile duct cancer). Infectivity of the parasite in the snail host is strongly correlated with destruction of helminths by the snail’s innate immune system, composed of cellular (hemocyte) and humoral (plasma) defense factors. To better understand this important host-parasite interface we applied sequential window acquisition of all theoretical spectra mass spectrometry (SWATH-MS) to identify and quantify the proteins from the hemolymph of B. siamensis goniomphalos experimentally infected with O. viverrini and compare them to non-infected snails (control group). A total of 362 and 242 proteins were identified in the hemocytes and plasma, respectively. Of these, 145 and 117 proteins exhibited significant differences in expression upon fluke infection in hemocytes and plasma, respectively. Among the proteins with significantly different expression patterns, we found proteins related to immune response (up-regulated in both hemocyte and plasma of infected snails) and proteins belonging to the structural and motor group (mostly down-regulated in hemocytes but up-regulated in plasma of infected snails). The proteins identified and quantified in this work will provide important information for the understanding of the factors involved in snail defense against O. viverrini and might facilitate the development of new strategies to control O. viverrini infection in endemic areas.
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spelling pubmed-51255712016-12-15 Differential Protein Expression in the Hemolymph of Bithynia siamensis goniomphalos Infected with Opisthorchis viverrini Suwannatrai, Kulwadee Suwannatrai, Apiporn Tabsripair, Pairat Welbat, Jariya Umka Tangkawattana, Sirikachorn Cantacessi, Cinzia Mulvenna, Jason Tesana, Smarn Loukas, Alex Sotillo, Javier PLoS Negl Trop Dis Research Article Bithynia siamensis goniomphalos is a freshwater snail that serves as the first intermediate host of the human liver fluke Opisthorchis viverrini. This parasite is a major public health problem in different countries throughout the Greater Mekong sub-region (Thailand, southern Vietnam, Lao PDR and Cambodia). Chronic O. viverrini infection also results in a gradual increase of fibrotic tissues in the biliary tract that are associated with hepatobiliary diseases and contribute to cholangiocarcinoma (a fatal type of bile duct cancer). Infectivity of the parasite in the snail host is strongly correlated with destruction of helminths by the snail’s innate immune system, composed of cellular (hemocyte) and humoral (plasma) defense factors. To better understand this important host-parasite interface we applied sequential window acquisition of all theoretical spectra mass spectrometry (SWATH-MS) to identify and quantify the proteins from the hemolymph of B. siamensis goniomphalos experimentally infected with O. viverrini and compare them to non-infected snails (control group). A total of 362 and 242 proteins were identified in the hemocytes and plasma, respectively. Of these, 145 and 117 proteins exhibited significant differences in expression upon fluke infection in hemocytes and plasma, respectively. Among the proteins with significantly different expression patterns, we found proteins related to immune response (up-regulated in both hemocyte and plasma of infected snails) and proteins belonging to the structural and motor group (mostly down-regulated in hemocytes but up-regulated in plasma of infected snails). The proteins identified and quantified in this work will provide important information for the understanding of the factors involved in snail defense against O. viverrini and might facilitate the development of new strategies to control O. viverrini infection in endemic areas. Public Library of Science 2016-11-28 /pmc/articles/PMC5125571/ /pubmed/27893749 http://dx.doi.org/10.1371/journal.pntd.0005104 Text en © 2016 Suwannatrai et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Suwannatrai, Kulwadee
Suwannatrai, Apiporn
Tabsripair, Pairat
Welbat, Jariya Umka
Tangkawattana, Sirikachorn
Cantacessi, Cinzia
Mulvenna, Jason
Tesana, Smarn
Loukas, Alex
Sotillo, Javier
Differential Protein Expression in the Hemolymph of Bithynia siamensis goniomphalos Infected with Opisthorchis viverrini
title Differential Protein Expression in the Hemolymph of Bithynia siamensis goniomphalos Infected with Opisthorchis viverrini
title_full Differential Protein Expression in the Hemolymph of Bithynia siamensis goniomphalos Infected with Opisthorchis viverrini
title_fullStr Differential Protein Expression in the Hemolymph of Bithynia siamensis goniomphalos Infected with Opisthorchis viverrini
title_full_unstemmed Differential Protein Expression in the Hemolymph of Bithynia siamensis goniomphalos Infected with Opisthorchis viverrini
title_short Differential Protein Expression in the Hemolymph of Bithynia siamensis goniomphalos Infected with Opisthorchis viverrini
title_sort differential protein expression in the hemolymph of bithynia siamensis goniomphalos infected with opisthorchis viverrini
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5125571/
https://www.ncbi.nlm.nih.gov/pubmed/27893749
http://dx.doi.org/10.1371/journal.pntd.0005104
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