Cargando…
Stable Epigenetic Variants Selected from an Induced Hypomethylated Fragaria vesca Population
Epigenetic inheritance was transmitted through selection over five generations of extreme early, but not late flowering time phenotypic lines in Fragaria vesca. Epigenetic variation was initially artificially induced using the DNA demethylation reagent 5-azacytidine (5-azaC). It is the first report...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5126047/ https://www.ncbi.nlm.nih.gov/pubmed/27965682 http://dx.doi.org/10.3389/fpls.2016.01768 |
_version_ | 1782470046331699200 |
---|---|
author | Xu, Jihua Tanino, Karen K. Robinson, Stephen J. |
author_facet | Xu, Jihua Tanino, Karen K. Robinson, Stephen J. |
author_sort | Xu, Jihua |
collection | PubMed |
description | Epigenetic inheritance was transmitted through selection over five generations of extreme early, but not late flowering time phenotypic lines in Fragaria vesca. Epigenetic variation was initially artificially induced using the DNA demethylation reagent 5-azacytidine (5-azaC). It is the first report to explore epigenetic variant selection and phenotypic trait inheritance in strawberry. Transmission frequency of these traits was determined across generations. The early flowering (EF4) and late stolon (LS) phenotypic traits were successfully transmitted across five and three generations through meiosis, respectively. Stable mitotic transmission of the early flowering phenotype was also demonstrated using clonal daughters derived from the 4th Generation (S4) mother plant. In order to further explore the DNA methylation patterns underlying the early flowering trait, the standard MSAP method using isoschizomers Hpa II/Msp I, and newly modified MSAP method using isoschizomers Tfi I/Pfe I which detected DNA methylation at CG, CHG, CHH sites were used in two early flowering lines, EF lines 1 (P2) and EF lines 2 (P3), and control lines (P1). A significant reduction in the number of fully-methylated bands was detected in P2 and P3 when compared to P1 using the novel MSAP method. In the standard MSAP, the symmetric CG and CHG methylation was maintained over generations in the early flowering lines based on the clustering in P2 and P3, the novel MSAP approach revealed the asymmetric CHH methylation pattern was not maintained over generations. This study provides evidence of stable selection of phenotypic traits, particularly early flowering through both meiosis and mitosis, which is meaningful to both breeding programs and commercial horticulture. The maintenance in CG and CHG methylation over generations suggests the early flowering phenotype might be related to DNA methylation alterations at the CG or CHG sites. Finally, this work provides a new approach for studying the role of epigenetics on complex quantitative trait improvement in strawberry, as well as providing a tool to expand phenotypic diversity and expedite potential new horticulture cultivar releases through either seed or vegetative propagation. |
format | Online Article Text |
id | pubmed-5126047 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-51260472016-12-13 Stable Epigenetic Variants Selected from an Induced Hypomethylated Fragaria vesca Population Xu, Jihua Tanino, Karen K. Robinson, Stephen J. Front Plant Sci Plant Science Epigenetic inheritance was transmitted through selection over five generations of extreme early, but not late flowering time phenotypic lines in Fragaria vesca. Epigenetic variation was initially artificially induced using the DNA demethylation reagent 5-azacytidine (5-azaC). It is the first report to explore epigenetic variant selection and phenotypic trait inheritance in strawberry. Transmission frequency of these traits was determined across generations. The early flowering (EF4) and late stolon (LS) phenotypic traits were successfully transmitted across five and three generations through meiosis, respectively. Stable mitotic transmission of the early flowering phenotype was also demonstrated using clonal daughters derived from the 4th Generation (S4) mother plant. In order to further explore the DNA methylation patterns underlying the early flowering trait, the standard MSAP method using isoschizomers Hpa II/Msp I, and newly modified MSAP method using isoschizomers Tfi I/Pfe I which detected DNA methylation at CG, CHG, CHH sites were used in two early flowering lines, EF lines 1 (P2) and EF lines 2 (P3), and control lines (P1). A significant reduction in the number of fully-methylated bands was detected in P2 and P3 when compared to P1 using the novel MSAP method. In the standard MSAP, the symmetric CG and CHG methylation was maintained over generations in the early flowering lines based on the clustering in P2 and P3, the novel MSAP approach revealed the asymmetric CHH methylation pattern was not maintained over generations. This study provides evidence of stable selection of phenotypic traits, particularly early flowering through both meiosis and mitosis, which is meaningful to both breeding programs and commercial horticulture. The maintenance in CG and CHG methylation over generations suggests the early flowering phenotype might be related to DNA methylation alterations at the CG or CHG sites. Finally, this work provides a new approach for studying the role of epigenetics on complex quantitative trait improvement in strawberry, as well as providing a tool to expand phenotypic diversity and expedite potential new horticulture cultivar releases through either seed or vegetative propagation. Frontiers Media S.A. 2016-11-29 /pmc/articles/PMC5126047/ /pubmed/27965682 http://dx.doi.org/10.3389/fpls.2016.01768 Text en Copyright © 2016 Xu, Tanino and Robinson. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Xu, Jihua Tanino, Karen K. Robinson, Stephen J. Stable Epigenetic Variants Selected from an Induced Hypomethylated Fragaria vesca Population |
title | Stable Epigenetic Variants Selected from an Induced Hypomethylated Fragaria vesca Population |
title_full | Stable Epigenetic Variants Selected from an Induced Hypomethylated Fragaria vesca Population |
title_fullStr | Stable Epigenetic Variants Selected from an Induced Hypomethylated Fragaria vesca Population |
title_full_unstemmed | Stable Epigenetic Variants Selected from an Induced Hypomethylated Fragaria vesca Population |
title_short | Stable Epigenetic Variants Selected from an Induced Hypomethylated Fragaria vesca Population |
title_sort | stable epigenetic variants selected from an induced hypomethylated fragaria vesca population |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5126047/ https://www.ncbi.nlm.nih.gov/pubmed/27965682 http://dx.doi.org/10.3389/fpls.2016.01768 |
work_keys_str_mv | AT xujihua stableepigeneticvariantsselectedfromaninducedhypomethylatedfragariavescapopulation AT taninokarenk stableepigeneticvariantsselectedfromaninducedhypomethylatedfragariavescapopulation AT robinsonstephenj stableepigeneticvariantsselectedfromaninducedhypomethylatedfragariavescapopulation |