Cargando…

Stability of targeted metabolite profiles of urine samples under different storage conditions

INTRODUCTION: Few studies have investigated the influence of storage conditions on urine samples and none of them used targeted mass spectrometry (MS). OBJECTIVES: We investigated the stability of metabolite profiles in urine samples under different storage conditions using targeted metabolomics. ME...

Descripción completa

Detalles Bibliográficos
Autores principales: Rotter, Markus, Brandmaier, Stefan, Prehn, Cornelia, Adam, Jonathan, Rabstein, Sylvia, Gawrych, Katarzyna, Brüning, Thomas, Illig, Thomas, Lickert, Heiko, Adamski, Jerzy, Wang-Sattler, Rui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5126183/
https://www.ncbi.nlm.nih.gov/pubmed/27980503
http://dx.doi.org/10.1007/s11306-016-1137-z
_version_ 1782470076642885632
author Rotter, Markus
Brandmaier, Stefan
Prehn, Cornelia
Adam, Jonathan
Rabstein, Sylvia
Gawrych, Katarzyna
Brüning, Thomas
Illig, Thomas
Lickert, Heiko
Adamski, Jerzy
Wang-Sattler, Rui
author_facet Rotter, Markus
Brandmaier, Stefan
Prehn, Cornelia
Adam, Jonathan
Rabstein, Sylvia
Gawrych, Katarzyna
Brüning, Thomas
Illig, Thomas
Lickert, Heiko
Adamski, Jerzy
Wang-Sattler, Rui
author_sort Rotter, Markus
collection PubMed
description INTRODUCTION: Few studies have investigated the influence of storage conditions on urine samples and none of them used targeted mass spectrometry (MS). OBJECTIVES: We investigated the stability of metabolite profiles in urine samples under different storage conditions using targeted metabolomics. METHODS: Pooled, fasting urine samples were collected and stored at −80 °C (biobank standard), −20 °C (freezer), 4 °C (fridge), ~9 °C (cool pack), and ~20 °C (room temperature) for 0, 2, 8 and 24 h. Metabolite concentrations were quantified with MS using the AbsoluteIDQ™ p150 assay. We used the Welch-Satterthwaite-test to compare the concentrations of each metabolite. Mixed effects linear regression was used to assess the influence of the interaction of storage time and temperature. RESULTS: The concentrations of 63 investigated metabolites were stable at −20 and 4 °C for up to 24 h when compared to samples immediately stored at −80 °C. When stored at ~9 °C for 24 h, few amino acids (Arg, Val and Leu/Ile) significantly decreased by 40% in concentration (P < 7.9E−04); for an additional three metabolites (Ser, Met, Hexose H1) when stored at ~20 °C reduced up to 60% in concentrations. The concentrations of four more metabolites (Glu, Phe, Pro, and Thr) were found to be significantly influenced when considering the interaction between exposure time and temperature. CONCLUSION: Our findings indicate that 78% of quantified metabolites were stable for all examined storage conditions. Particularly, some amino acid concentrations were sensitive to changes after prolonged storage at room temperature. Shipping or storing urine samples on cool packs or at room temperature for more than 8 h and multiple numbers of freeze and thaw cycles should be avoided. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11306-016-1137-z) contains supplementary material, which is available to authorized users.
format Online
Article
Text
id pubmed-5126183
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Springer US
record_format MEDLINE/PubMed
spelling pubmed-51261832016-12-13 Stability of targeted metabolite profiles of urine samples under different storage conditions Rotter, Markus Brandmaier, Stefan Prehn, Cornelia Adam, Jonathan Rabstein, Sylvia Gawrych, Katarzyna Brüning, Thomas Illig, Thomas Lickert, Heiko Adamski, Jerzy Wang-Sattler, Rui Metabolomics Original Article INTRODUCTION: Few studies have investigated the influence of storage conditions on urine samples and none of them used targeted mass spectrometry (MS). OBJECTIVES: We investigated the stability of metabolite profiles in urine samples under different storage conditions using targeted metabolomics. METHODS: Pooled, fasting urine samples were collected and stored at −80 °C (biobank standard), −20 °C (freezer), 4 °C (fridge), ~9 °C (cool pack), and ~20 °C (room temperature) for 0, 2, 8 and 24 h. Metabolite concentrations were quantified with MS using the AbsoluteIDQ™ p150 assay. We used the Welch-Satterthwaite-test to compare the concentrations of each metabolite. Mixed effects linear regression was used to assess the influence of the interaction of storage time and temperature. RESULTS: The concentrations of 63 investigated metabolites were stable at −20 and 4 °C for up to 24 h when compared to samples immediately stored at −80 °C. When stored at ~9 °C for 24 h, few amino acids (Arg, Val and Leu/Ile) significantly decreased by 40% in concentration (P < 7.9E−04); for an additional three metabolites (Ser, Met, Hexose H1) when stored at ~20 °C reduced up to 60% in concentrations. The concentrations of four more metabolites (Glu, Phe, Pro, and Thr) were found to be significantly influenced when considering the interaction between exposure time and temperature. CONCLUSION: Our findings indicate that 78% of quantified metabolites were stable for all examined storage conditions. Particularly, some amino acid concentrations were sensitive to changes after prolonged storage at room temperature. Shipping or storing urine samples on cool packs or at room temperature for more than 8 h and multiple numbers of freeze and thaw cycles should be avoided. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11306-016-1137-z) contains supplementary material, which is available to authorized users. Springer US 2016-11-28 2017 /pmc/articles/PMC5126183/ /pubmed/27980503 http://dx.doi.org/10.1007/s11306-016-1137-z Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Rotter, Markus
Brandmaier, Stefan
Prehn, Cornelia
Adam, Jonathan
Rabstein, Sylvia
Gawrych, Katarzyna
Brüning, Thomas
Illig, Thomas
Lickert, Heiko
Adamski, Jerzy
Wang-Sattler, Rui
Stability of targeted metabolite profiles of urine samples under different storage conditions
title Stability of targeted metabolite profiles of urine samples under different storage conditions
title_full Stability of targeted metabolite profiles of urine samples under different storage conditions
title_fullStr Stability of targeted metabolite profiles of urine samples under different storage conditions
title_full_unstemmed Stability of targeted metabolite profiles of urine samples under different storage conditions
title_short Stability of targeted metabolite profiles of urine samples under different storage conditions
title_sort stability of targeted metabolite profiles of urine samples under different storage conditions
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5126183/
https://www.ncbi.nlm.nih.gov/pubmed/27980503
http://dx.doi.org/10.1007/s11306-016-1137-z
work_keys_str_mv AT rottermarkus stabilityoftargetedmetaboliteprofilesofurinesamplesunderdifferentstorageconditions
AT brandmaierstefan stabilityoftargetedmetaboliteprofilesofurinesamplesunderdifferentstorageconditions
AT prehncornelia stabilityoftargetedmetaboliteprofilesofurinesamplesunderdifferentstorageconditions
AT adamjonathan stabilityoftargetedmetaboliteprofilesofurinesamplesunderdifferentstorageconditions
AT rabsteinsylvia stabilityoftargetedmetaboliteprofilesofurinesamplesunderdifferentstorageconditions
AT gawrychkatarzyna stabilityoftargetedmetaboliteprofilesofurinesamplesunderdifferentstorageconditions
AT bruningthomas stabilityoftargetedmetaboliteprofilesofurinesamplesunderdifferentstorageconditions
AT illigthomas stabilityoftargetedmetaboliteprofilesofurinesamplesunderdifferentstorageconditions
AT lickertheiko stabilityoftargetedmetaboliteprofilesofurinesamplesunderdifferentstorageconditions
AT adamskijerzy stabilityoftargetedmetaboliteprofilesofurinesamplesunderdifferentstorageconditions
AT wangsattlerrui stabilityoftargetedmetaboliteprofilesofurinesamplesunderdifferentstorageconditions