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Extending the Study of the 6,8 Rearrangement in Flavylium Compounds to Higher pH Values: Interconversion between 6‐Bromo and 8‐Bromo‐apigeninidin

The rearrangement between isomers 6‐ and 8‐bromo‐apigeninidin (6 and 8) was studied by pH jumps using stopped flow, UV/Vis, NMR, and HPLC analysis. The system constitutes a pH‐dependent network of chemical reactions involving up to 18 different species. The dynamic network is equivalent to a single...

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Autores principales: Cruz, Luís M., Basílio, Nuno M., de Freitas, Victor A., Lima, João C., Pina, Fernando J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5126205/
https://www.ncbi.nlm.nih.gov/pubmed/27933227
http://dx.doi.org/10.1002/open.201500210
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author Cruz, Luís M.
Basílio, Nuno M.
de Freitas, Victor A.
Lima, João C.
Pina, Fernando J.
author_facet Cruz, Luís M.
Basílio, Nuno M.
de Freitas, Victor A.
Lima, João C.
Pina, Fernando J.
author_sort Cruz, Luís M.
collection PubMed
description The rearrangement between isomers 6‐ and 8‐bromo‐apigeninidin (6 and 8) was studied by pH jumps using stopped flow, UV/Vis, NMR, and HPLC analysis. The system constitutes a pH‐dependent network of chemical reactions involving up to 18 different species. The dynamic network is equivalent to a single diprotic acid exhibiting two pK (a)s, 2.55 and 5.4. Similar to other flavylium derivatives, the mole fraction of the species hemiketal and cis‐chalcone in both multistate isomers are negligible at the equilibrium. At pH 1, the pure isomers are slowly converted in a mixture containing about 50 % of isomers 6 and 8, while at pH 4, the system evolves to mixture of 10 % trans‐chalcone and 90 % of quinoidal bases. A series of pH jumps from pure isomer 6 at pH 1 to pH 6 and back to pH 1 leads to the same initial absorption spectra of the pure isomer 6. The same occurs for pure isomer 8, showing the lack of communication between the cis‐chalcones, at least in the time scale of few minutes. A pH jump from the equilibrated mixture of the isomers at pH 1.0 to 5.8 permits to follow a very slow isomerization.
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spelling pubmed-51262052016-12-08 Extending the Study of the 6,8 Rearrangement in Flavylium Compounds to Higher pH Values: Interconversion between 6‐Bromo and 8‐Bromo‐apigeninidin Cruz, Luís M. Basílio, Nuno M. de Freitas, Victor A. Lima, João C. Pina, Fernando J. ChemistryOpen Full Papers The rearrangement between isomers 6‐ and 8‐bromo‐apigeninidin (6 and 8) was studied by pH jumps using stopped flow, UV/Vis, NMR, and HPLC analysis. The system constitutes a pH‐dependent network of chemical reactions involving up to 18 different species. The dynamic network is equivalent to a single diprotic acid exhibiting two pK (a)s, 2.55 and 5.4. Similar to other flavylium derivatives, the mole fraction of the species hemiketal and cis‐chalcone in both multistate isomers are negligible at the equilibrium. At pH 1, the pure isomers are slowly converted in a mixture containing about 50 % of isomers 6 and 8, while at pH 4, the system evolves to mixture of 10 % trans‐chalcone and 90 % of quinoidal bases. A series of pH jumps from pure isomer 6 at pH 1 to pH 6 and back to pH 1 leads to the same initial absorption spectra of the pure isomer 6. The same occurs for pure isomer 8, showing the lack of communication between the cis‐chalcones, at least in the time scale of few minutes. A pH jump from the equilibrated mixture of the isomers at pH 1.0 to 5.8 permits to follow a very slow isomerization. John Wiley and Sons Inc. 2016-02-25 /pmc/articles/PMC5126205/ /pubmed/27933227 http://dx.doi.org/10.1002/open.201500210 Text en © 2016 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs (http://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Full Papers
Cruz, Luís M.
Basílio, Nuno M.
de Freitas, Victor A.
Lima, João C.
Pina, Fernando J.
Extending the Study of the 6,8 Rearrangement in Flavylium Compounds to Higher pH Values: Interconversion between 6‐Bromo and 8‐Bromo‐apigeninidin
title Extending the Study of the 6,8 Rearrangement in Flavylium Compounds to Higher pH Values: Interconversion between 6‐Bromo and 8‐Bromo‐apigeninidin
title_full Extending the Study of the 6,8 Rearrangement in Flavylium Compounds to Higher pH Values: Interconversion between 6‐Bromo and 8‐Bromo‐apigeninidin
title_fullStr Extending the Study of the 6,8 Rearrangement in Flavylium Compounds to Higher pH Values: Interconversion between 6‐Bromo and 8‐Bromo‐apigeninidin
title_full_unstemmed Extending the Study of the 6,8 Rearrangement in Flavylium Compounds to Higher pH Values: Interconversion between 6‐Bromo and 8‐Bromo‐apigeninidin
title_short Extending the Study of the 6,8 Rearrangement in Flavylium Compounds to Higher pH Values: Interconversion between 6‐Bromo and 8‐Bromo‐apigeninidin
title_sort extending the study of the 6,8 rearrangement in flavylium compounds to higher ph values: interconversion between 6‐bromo and 8‐bromo‐apigeninidin
topic Full Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5126205/
https://www.ncbi.nlm.nih.gov/pubmed/27933227
http://dx.doi.org/10.1002/open.201500210
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