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Involvement of microRNA-133 and -29 in cardiac disturbances in diabetic ovariectomized rats
OBJECTIVE(S): Menopause and diabetes obviously increase the risk of cardiovascular disease in women. The aims of the present study were to evaluate the effects of ovariectomy in type 2 diabetes on the histology and expression of miRNA-29, miRNA-133, IGF-1 and Bcl-2 genes and Bcl-2 protein and caspas...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Mashhad University of Medical Sciences
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5126218/ https://www.ncbi.nlm.nih.gov/pubmed/27917273 |
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author | Habibi, Parisa Alihemmati, Alireza Nasirzadeh, Mohammadreza Yousefi, Hadi Habibi, Mohammadrasoul Ahmadiasl, Nasser |
author_facet | Habibi, Parisa Alihemmati, Alireza Nasirzadeh, Mohammadreza Yousefi, Hadi Habibi, Mohammadrasoul Ahmadiasl, Nasser |
author_sort | Habibi, Parisa |
collection | PubMed |
description | OBJECTIVE(S): Menopause and diabetes obviously increase the risk of cardiovascular disease in women. The aims of the present study were to evaluate the effects of ovariectomy in type 2 diabetes on the histology and expression of miRNA-29, miRNA-133, IGF-1 and Bcl-2 genes and Bcl-2 protein and caspase 3 activity in the hearts of female rats. MATERIALS AND METHODS: Forty Female Wistar rats were divided into four groups: control, sham, ovariectomized (OVX), and ovariectomized with type 2 diabetes (OVX.D). After the 8-week experiment, the histological evaluation of the heart tissue was performed using H&E staining and PAS analysis, and cardiac expression of miRNA-29, miRNA-133, IGF-1, and Bcl-2 were evaluated using real-time PCR, and Bcl-2 protein and caspase 3 activity were evaluated using Western blot and ELISA. RESULTS: Ovariectomy significantly decreased miRNA-29, miRNA-133, IGF-1, and BCL-2 expression and Bcl-2 protein and increased caspase 3 activity in the heart compared to sham animals group (P<0.05). Type 2 diabetes in ovariectomized rats markedly decreased expression of miRNA-29, miRNA-133, IGF-1, BCL-2 genes, and Bcl-2 protein, and increased caspase 3 activity and reduced collagen and fibroblast tissue and glycogen granule deposition in relation to OVX group (P<0.05). CONCLUSION: Our findings suggest that type 2 diabetes and menopause synergically could enhance the cardiac fibrosis through dysregulation of miRNA-29, miRNA-133, IGF-1, and Bcl-2 genes expression and Bcl-2 protein and upregulation of caspase 3 activity. |
format | Online Article Text |
id | pubmed-5126218 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Mashhad University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-51262182016-12-02 Involvement of microRNA-133 and -29 in cardiac disturbances in diabetic ovariectomized rats Habibi, Parisa Alihemmati, Alireza Nasirzadeh, Mohammadreza Yousefi, Hadi Habibi, Mohammadrasoul Ahmadiasl, Nasser Iran J Basic Med Sci Original Article OBJECTIVE(S): Menopause and diabetes obviously increase the risk of cardiovascular disease in women. The aims of the present study were to evaluate the effects of ovariectomy in type 2 diabetes on the histology and expression of miRNA-29, miRNA-133, IGF-1 and Bcl-2 genes and Bcl-2 protein and caspase 3 activity in the hearts of female rats. MATERIALS AND METHODS: Forty Female Wistar rats were divided into four groups: control, sham, ovariectomized (OVX), and ovariectomized with type 2 diabetes (OVX.D). After the 8-week experiment, the histological evaluation of the heart tissue was performed using H&E staining and PAS analysis, and cardiac expression of miRNA-29, miRNA-133, IGF-1, and Bcl-2 were evaluated using real-time PCR, and Bcl-2 protein and caspase 3 activity were evaluated using Western blot and ELISA. RESULTS: Ovariectomy significantly decreased miRNA-29, miRNA-133, IGF-1, and BCL-2 expression and Bcl-2 protein and increased caspase 3 activity in the heart compared to sham animals group (P<0.05). Type 2 diabetes in ovariectomized rats markedly decreased expression of miRNA-29, miRNA-133, IGF-1, BCL-2 genes, and Bcl-2 protein, and increased caspase 3 activity and reduced collagen and fibroblast tissue and glycogen granule deposition in relation to OVX group (P<0.05). CONCLUSION: Our findings suggest that type 2 diabetes and menopause synergically could enhance the cardiac fibrosis through dysregulation of miRNA-29, miRNA-133, IGF-1, and Bcl-2 genes expression and Bcl-2 protein and upregulation of caspase 3 activity. Mashhad University of Medical Sciences 2016-11 /pmc/articles/PMC5126218/ /pubmed/27917273 Text en Copyright: © Iranian Journal of Basic Medical Sciences http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Habibi, Parisa Alihemmati, Alireza Nasirzadeh, Mohammadreza Yousefi, Hadi Habibi, Mohammadrasoul Ahmadiasl, Nasser Involvement of microRNA-133 and -29 in cardiac disturbances in diabetic ovariectomized rats |
title | Involvement of microRNA-133 and -29 in cardiac disturbances in diabetic ovariectomized rats |
title_full | Involvement of microRNA-133 and -29 in cardiac disturbances in diabetic ovariectomized rats |
title_fullStr | Involvement of microRNA-133 and -29 in cardiac disturbances in diabetic ovariectomized rats |
title_full_unstemmed | Involvement of microRNA-133 and -29 in cardiac disturbances in diabetic ovariectomized rats |
title_short | Involvement of microRNA-133 and -29 in cardiac disturbances in diabetic ovariectomized rats |
title_sort | involvement of microrna-133 and -29 in cardiac disturbances in diabetic ovariectomized rats |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5126218/ https://www.ncbi.nlm.nih.gov/pubmed/27917273 |
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