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Sertoli cell condition medium can induce germ like cells from bone marrow derived mesenchymal stem cells
OBJECTIVE(S): Although many researchers have confirmed induction of germ cells from bone marrow mesenchymal stem cells (BMMSCs), there are no reports that confirm spontaneous differentiation of germ cells from BMMSCs. In this study, we have evaluated the effect of adult Sertoli cell condition medium...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Mashhad University of Medical Sciences
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5126219/ https://www.ncbi.nlm.nih.gov/pubmed/27917274 |
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author | Monfared, Mahdieh Hajian Minaee, Bagher Rastegar, Tayebeh Khrazinejad, Ebrahim Barbarestani, Mohammad |
author_facet | Monfared, Mahdieh Hajian Minaee, Bagher Rastegar, Tayebeh Khrazinejad, Ebrahim Barbarestani, Mohammad |
author_sort | Monfared, Mahdieh Hajian |
collection | PubMed |
description | OBJECTIVE(S): Although many researchers have confirmed induction of germ cells from bone marrow mesenchymal stem cells (BMMSCs), there are no reports that confirm spontaneous differentiation of germ cells from BMMSCs. In this study, we have evaluated the effect of adult Sertoli cell condition medium (SCCM) as a mutative factor in the induction of germ cells from BMMSCs. MATERIALS AND METHODS: BMMSCs were collected from the bone marrow of 6-8-week old NMRI mice and their mesenchymal entities were proven using superficial markers (expression of CD44 and CD73 and non-expresion of CD45 and CD11b) by fow cytometry. Their multi-potential entities were proved with differentiation to osteogenic and adipogenic cells for 21 days. Also isolated Sertoli cells were enriched using lectin coated plates and Sertoli cell condition medium (SCCM) was collected. Sertoli cells were identified by immunocytochemistry and Vimentin marker. The cells were then differentiated into germ cells with SCCM for 2 weeks. Finally induced cells were evaluated by RT-PCR and immunocytochemistry. RESULTS: Differentiation of mesenchymal stem cells to osteoblast and adipocyte showed their multi-potential property. Expression of CD44 and CD73 and non-expression of CD45 and CD11b confirmed mesenchyme cells. Immunocytochemistry and RT-PCR results showed expression of germ cells specific marker (Mvh). CONCLUSION: This study confirmed the effect of SCCM as a motivational factor that can used for differentiation of germ cells from BMMSCs. |
format | Online Article Text |
id | pubmed-5126219 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Mashhad University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-51262192016-12-02 Sertoli cell condition medium can induce germ like cells from bone marrow derived mesenchymal stem cells Monfared, Mahdieh Hajian Minaee, Bagher Rastegar, Tayebeh Khrazinejad, Ebrahim Barbarestani, Mohammad Iran J Basic Med Sci Original Article OBJECTIVE(S): Although many researchers have confirmed induction of germ cells from bone marrow mesenchymal stem cells (BMMSCs), there are no reports that confirm spontaneous differentiation of germ cells from BMMSCs. In this study, we have evaluated the effect of adult Sertoli cell condition medium (SCCM) as a mutative factor in the induction of germ cells from BMMSCs. MATERIALS AND METHODS: BMMSCs were collected from the bone marrow of 6-8-week old NMRI mice and their mesenchymal entities were proven using superficial markers (expression of CD44 and CD73 and non-expresion of CD45 and CD11b) by fow cytometry. Their multi-potential entities were proved with differentiation to osteogenic and adipogenic cells for 21 days. Also isolated Sertoli cells were enriched using lectin coated plates and Sertoli cell condition medium (SCCM) was collected. Sertoli cells were identified by immunocytochemistry and Vimentin marker. The cells were then differentiated into germ cells with SCCM for 2 weeks. Finally induced cells were evaluated by RT-PCR and immunocytochemistry. RESULTS: Differentiation of mesenchymal stem cells to osteoblast and adipocyte showed their multi-potential property. Expression of CD44 and CD73 and non-expression of CD45 and CD11b confirmed mesenchyme cells. Immunocytochemistry and RT-PCR results showed expression of germ cells specific marker (Mvh). CONCLUSION: This study confirmed the effect of SCCM as a motivational factor that can used for differentiation of germ cells from BMMSCs. Mashhad University of Medical Sciences 2016-11 /pmc/articles/PMC5126219/ /pubmed/27917274 Text en Copyright: © Iranian Journal of Basic Medical Sciences http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Monfared, Mahdieh Hajian Minaee, Bagher Rastegar, Tayebeh Khrazinejad, Ebrahim Barbarestani, Mohammad Sertoli cell condition medium can induce germ like cells from bone marrow derived mesenchymal stem cells |
title | Sertoli cell condition medium can induce germ like cells from bone marrow derived mesenchymal stem cells |
title_full | Sertoli cell condition medium can induce germ like cells from bone marrow derived mesenchymal stem cells |
title_fullStr | Sertoli cell condition medium can induce germ like cells from bone marrow derived mesenchymal stem cells |
title_full_unstemmed | Sertoli cell condition medium can induce germ like cells from bone marrow derived mesenchymal stem cells |
title_short | Sertoli cell condition medium can induce germ like cells from bone marrow derived mesenchymal stem cells |
title_sort | sertoli cell condition medium can induce germ like cells from bone marrow derived mesenchymal stem cells |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5126219/ https://www.ncbi.nlm.nih.gov/pubmed/27917274 |
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