Cargando…

Synergistic effect of Thymbra spicata L. extracts with antibiotics against multidrug- resistant Staphylococcus aureus and Klebsiella pneumoniae strains

OBJECTIVE(S): To evaluate the in vitro interaction between different extracts of Thymbra spicata L. and certain antimicrobial drugs of different mechanisms, including ampicillin, cefotaxime, amikacin and ciprofloxacin. This study was performed against multidrug-resistant strains of Staphylococcus au...

Descripción completa

Detalles Bibliográficos
Autores principales: Haroun, Mohammad F, Al-Kayali, Rawaa S
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mashhad University of Medical Sciences 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5126220/
https://www.ncbi.nlm.nih.gov/pubmed/27917275
_version_ 1782470084923490304
author Haroun, Mohammad F
Al-Kayali, Rawaa S
author_facet Haroun, Mohammad F
Al-Kayali, Rawaa S
author_sort Haroun, Mohammad F
collection PubMed
description OBJECTIVE(S): To evaluate the in vitro interaction between different extracts of Thymbra spicata L. and certain antimicrobial drugs of different mechanisms, including ampicillin, cefotaxime, amikacin and ciprofloxacin. This study was performed against multidrug-resistant strains of Staphylococcus aureus and Klebsiella pneumoniae. MATERIALS AND METHODS: Evaluation of antibacterial activity and synergy interaction between plant extracts and antimicrobial agents was carried out using checkerboard microdilution. RESULTS: Different interactions (synergistic, additive and indifference) were observed between plant crude extracts and used antibiotics depending on the strain. The fractional inhibitory concentration (FIC) index ranged from 0.02 to 1.5 for S. aureus and 0.25 to 2 for K. pneumoniae strains. The best synergistic capacity appeared with cefotaxime against S. aureus strains, where the activity of cefotaxime was increased from 8- to 128-fold. CONCLUSION: These results may indicate that T. spicata extracts potentiates the antimicrobial action of antibiotics, suggesting a possible utilization of this herb in combination therapy against emerging multidrug-resistance S. aureus and K. pneumoniae.
format Online
Article
Text
id pubmed-5126220
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Mashhad University of Medical Sciences
record_format MEDLINE/PubMed
spelling pubmed-51262202016-12-02 Synergistic effect of Thymbra spicata L. extracts with antibiotics against multidrug- resistant Staphylococcus aureus and Klebsiella pneumoniae strains Haroun, Mohammad F Al-Kayali, Rawaa S Iran J Basic Med Sci Original Article OBJECTIVE(S): To evaluate the in vitro interaction between different extracts of Thymbra spicata L. and certain antimicrobial drugs of different mechanisms, including ampicillin, cefotaxime, amikacin and ciprofloxacin. This study was performed against multidrug-resistant strains of Staphylococcus aureus and Klebsiella pneumoniae. MATERIALS AND METHODS: Evaluation of antibacterial activity and synergy interaction between plant extracts and antimicrobial agents was carried out using checkerboard microdilution. RESULTS: Different interactions (synergistic, additive and indifference) were observed between plant crude extracts and used antibiotics depending on the strain. The fractional inhibitory concentration (FIC) index ranged from 0.02 to 1.5 for S. aureus and 0.25 to 2 for K. pneumoniae strains. The best synergistic capacity appeared with cefotaxime against S. aureus strains, where the activity of cefotaxime was increased from 8- to 128-fold. CONCLUSION: These results may indicate that T. spicata extracts potentiates the antimicrobial action of antibiotics, suggesting a possible utilization of this herb in combination therapy against emerging multidrug-resistance S. aureus and K. pneumoniae. Mashhad University of Medical Sciences 2016-11 /pmc/articles/PMC5126220/ /pubmed/27917275 Text en Copyright: © Iranian Journal of Basic Medical Sciences http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Haroun, Mohammad F
Al-Kayali, Rawaa S
Synergistic effect of Thymbra spicata L. extracts with antibiotics against multidrug- resistant Staphylococcus aureus and Klebsiella pneumoniae strains
title Synergistic effect of Thymbra spicata L. extracts with antibiotics against multidrug- resistant Staphylococcus aureus and Klebsiella pneumoniae strains
title_full Synergistic effect of Thymbra spicata L. extracts with antibiotics against multidrug- resistant Staphylococcus aureus and Klebsiella pneumoniae strains
title_fullStr Synergistic effect of Thymbra spicata L. extracts with antibiotics against multidrug- resistant Staphylococcus aureus and Klebsiella pneumoniae strains
title_full_unstemmed Synergistic effect of Thymbra spicata L. extracts with antibiotics against multidrug- resistant Staphylococcus aureus and Klebsiella pneumoniae strains
title_short Synergistic effect of Thymbra spicata L. extracts with antibiotics against multidrug- resistant Staphylococcus aureus and Klebsiella pneumoniae strains
title_sort synergistic effect of thymbra spicata l. extracts with antibiotics against multidrug- resistant staphylococcus aureus and klebsiella pneumoniae strains
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5126220/
https://www.ncbi.nlm.nih.gov/pubmed/27917275
work_keys_str_mv AT harounmohammadf synergisticeffectofthymbraspicatalextractswithantibioticsagainstmultidrugresistantstaphylococcusaureusandklebsiellapneumoniaestrains
AT alkayalirawaas synergisticeffectofthymbraspicatalextractswithantibioticsagainstmultidrugresistantstaphylococcusaureusandklebsiellapneumoniaestrains