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The Suppressed Induction of Human Mature Cytotoxic T Lymphocytes Caused by Asbestos Is Not due to Interleukin-2 Insufficiency
We previously reported that exposure to chrysotile B (CB) asbestos suppressed the induction of mature cytotoxic T lymphocytes (CTLs) during mixed lymphocyte reaction assays (MLRs) with a decrease in the proliferation of immature CTLs. However, the mechanism responsible for the effect of asbestos fib...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Hindawi Publishing Corporation
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5126420/ https://www.ncbi.nlm.nih.gov/pubmed/27975069 http://dx.doi.org/10.1155/2016/7484872 |
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author | Kumagai-Takei, Naoko Nishimura, Yasumitsu Matsuzaki, Hidenori Lee, Suni Yoshitome, Kei Hayashi, Hiroaki Otsuki, Takemi |
author_facet | Kumagai-Takei, Naoko Nishimura, Yasumitsu Matsuzaki, Hidenori Lee, Suni Yoshitome, Kei Hayashi, Hiroaki Otsuki, Takemi |
author_sort | Kumagai-Takei, Naoko |
collection | PubMed |
description | We previously reported that exposure to chrysotile B (CB) asbestos suppressed the induction of mature cytotoxic T lymphocytes (CTLs) during mixed lymphocyte reaction assays (MLRs) with a decrease in the proliferation of immature CTLs. However, the mechanism responsible for the effect of asbestos fibers on the differentiation of CTLs remains unclear. Since interleukin-2 (IL-2) is a regulator of T lymphocyte proliferation, we examined the effect of IL-2 addition on suppressed CTL differentiation in CB-exposed cultures using flow cytometry (FCM). When IL-2 was added at 1 ng/mL on the second day of MLRs, the asbestos-caused decreases in the proliferation and percentages of CD25(+) and CD45RO(+) cells in CD8(+) lymphocytes were not recovered by IL-2 addition, although the decrease in percentage of granzyme B(+) cells was partially recovered. CD8(+) lymphocytes from the IL-2-treated culture with asbestos showed the same degree of cytotoxicity as those in cultures without IL-2 or asbestos. These findings indicate that IL-2 insufficiency is not the main cause for the suppressed induction of CTLs by asbestos exposure, although they suggest a potential for the improvement of such suppressed CTL functions. Secretory factors other than IL-2 in addition to membrane-bound stimulatory molecules may play a role in asbestos-caused suppressed CTL differentiation. |
format | Online Article Text |
id | pubmed-5126420 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-51264202016-12-14 The Suppressed Induction of Human Mature Cytotoxic T Lymphocytes Caused by Asbestos Is Not due to Interleukin-2 Insufficiency Kumagai-Takei, Naoko Nishimura, Yasumitsu Matsuzaki, Hidenori Lee, Suni Yoshitome, Kei Hayashi, Hiroaki Otsuki, Takemi J Immunol Res Research Article We previously reported that exposure to chrysotile B (CB) asbestos suppressed the induction of mature cytotoxic T lymphocytes (CTLs) during mixed lymphocyte reaction assays (MLRs) with a decrease in the proliferation of immature CTLs. However, the mechanism responsible for the effect of asbestos fibers on the differentiation of CTLs remains unclear. Since interleukin-2 (IL-2) is a regulator of T lymphocyte proliferation, we examined the effect of IL-2 addition on suppressed CTL differentiation in CB-exposed cultures using flow cytometry (FCM). When IL-2 was added at 1 ng/mL on the second day of MLRs, the asbestos-caused decreases in the proliferation and percentages of CD25(+) and CD45RO(+) cells in CD8(+) lymphocytes were not recovered by IL-2 addition, although the decrease in percentage of granzyme B(+) cells was partially recovered. CD8(+) lymphocytes from the IL-2-treated culture with asbestos showed the same degree of cytotoxicity as those in cultures without IL-2 or asbestos. These findings indicate that IL-2 insufficiency is not the main cause for the suppressed induction of CTLs by asbestos exposure, although they suggest a potential for the improvement of such suppressed CTL functions. Secretory factors other than IL-2 in addition to membrane-bound stimulatory molecules may play a role in asbestos-caused suppressed CTL differentiation. Hindawi Publishing Corporation 2016 2016-11-15 /pmc/articles/PMC5126420/ /pubmed/27975069 http://dx.doi.org/10.1155/2016/7484872 Text en Copyright © 2016 Naoko Kumagai-Takei et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Kumagai-Takei, Naoko Nishimura, Yasumitsu Matsuzaki, Hidenori Lee, Suni Yoshitome, Kei Hayashi, Hiroaki Otsuki, Takemi The Suppressed Induction of Human Mature Cytotoxic T Lymphocytes Caused by Asbestos Is Not due to Interleukin-2 Insufficiency |
title | The Suppressed Induction of Human Mature Cytotoxic T Lymphocytes Caused by Asbestos Is Not due to Interleukin-2 Insufficiency |
title_full | The Suppressed Induction of Human Mature Cytotoxic T Lymphocytes Caused by Asbestos Is Not due to Interleukin-2 Insufficiency |
title_fullStr | The Suppressed Induction of Human Mature Cytotoxic T Lymphocytes Caused by Asbestos Is Not due to Interleukin-2 Insufficiency |
title_full_unstemmed | The Suppressed Induction of Human Mature Cytotoxic T Lymphocytes Caused by Asbestos Is Not due to Interleukin-2 Insufficiency |
title_short | The Suppressed Induction of Human Mature Cytotoxic T Lymphocytes Caused by Asbestos Is Not due to Interleukin-2 Insufficiency |
title_sort | suppressed induction of human mature cytotoxic t lymphocytes caused by asbestos is not due to interleukin-2 insufficiency |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5126420/ https://www.ncbi.nlm.nih.gov/pubmed/27975069 http://dx.doi.org/10.1155/2016/7484872 |
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