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Principal function of mineralocorticoid signaling suggested by constitutive knockout of the mineralocorticoid receptor in medaka fish

As in osmoregulation, mineralocorticoid signaling is implicated in the control of brain-behavior actions. Nevertheless, the understanding of this role is limited, partly due to the mortality of mineralocorticoid receptor (MR)-knockout (KO) mice due to impaired Na(+) reabsorption. In teleost fish, a...

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Autores principales: Sakamoto, Tatsuya, Yoshiki, Madoka, Takahashi, Hideya, Yoshida, Masayuki, Ogino, Yukiko, Ikeuchi, Toshitaka, Nakamachi, Tomoya, Konno, Norifumi, Matsuda, Kouhei, Sakamoto, Hirotaka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5126551/
https://www.ncbi.nlm.nih.gov/pubmed/27897263
http://dx.doi.org/10.1038/srep37991
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author Sakamoto, Tatsuya
Yoshiki, Madoka
Takahashi, Hideya
Yoshida, Masayuki
Ogino, Yukiko
Ikeuchi, Toshitaka
Nakamachi, Tomoya
Konno, Norifumi
Matsuda, Kouhei
Sakamoto, Hirotaka
author_facet Sakamoto, Tatsuya
Yoshiki, Madoka
Takahashi, Hideya
Yoshida, Masayuki
Ogino, Yukiko
Ikeuchi, Toshitaka
Nakamachi, Tomoya
Konno, Norifumi
Matsuda, Kouhei
Sakamoto, Hirotaka
author_sort Sakamoto, Tatsuya
collection PubMed
description As in osmoregulation, mineralocorticoid signaling is implicated in the control of brain-behavior actions. Nevertheless, the understanding of this role is limited, partly due to the mortality of mineralocorticoid receptor (MR)-knockout (KO) mice due to impaired Na(+) reabsorption. In teleost fish, a distinct mineralocorticoid system has only been identified recently. Here, we generated a constitutive MR-KO medaka as the first adult-viable MR-KO animal, since MR expression is modest in osmoregulatory organs but high in the brain of adult medaka as for most teleosts. Hyper- and hypo-osmoregulation were normal in MR-KO medaka. When we studied the behavioral phenotypes based on the central MR localization, however, MR-KO medaka failed to track moving dots despite having an increase in acceleration of swimming. These findings reinforce previous results showing a minor role for mineralocorticoid signaling in fish osmoregulation, and provide the first convincing evidence that MR is required for normal locomotor activity in response to visual motion stimuli, but not for the recognition of these stimuli per se. We suggest that MR potentially integrates brain-behavioral and visual responses, which might be a conserved function of mineralocorticoid signaling through vertebrates. Importantly, this fish model allows for the possible identification of novel aspects of mineralocorticoid signaling.
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spelling pubmed-51265512016-12-08 Principal function of mineralocorticoid signaling suggested by constitutive knockout of the mineralocorticoid receptor in medaka fish Sakamoto, Tatsuya Yoshiki, Madoka Takahashi, Hideya Yoshida, Masayuki Ogino, Yukiko Ikeuchi, Toshitaka Nakamachi, Tomoya Konno, Norifumi Matsuda, Kouhei Sakamoto, Hirotaka Sci Rep Article As in osmoregulation, mineralocorticoid signaling is implicated in the control of brain-behavior actions. Nevertheless, the understanding of this role is limited, partly due to the mortality of mineralocorticoid receptor (MR)-knockout (KO) mice due to impaired Na(+) reabsorption. In teleost fish, a distinct mineralocorticoid system has only been identified recently. Here, we generated a constitutive MR-KO medaka as the first adult-viable MR-KO animal, since MR expression is modest in osmoregulatory organs but high in the brain of adult medaka as for most teleosts. Hyper- and hypo-osmoregulation were normal in MR-KO medaka. When we studied the behavioral phenotypes based on the central MR localization, however, MR-KO medaka failed to track moving dots despite having an increase in acceleration of swimming. These findings reinforce previous results showing a minor role for mineralocorticoid signaling in fish osmoregulation, and provide the first convincing evidence that MR is required for normal locomotor activity in response to visual motion stimuli, but not for the recognition of these stimuli per se. We suggest that MR potentially integrates brain-behavioral and visual responses, which might be a conserved function of mineralocorticoid signaling through vertebrates. Importantly, this fish model allows for the possible identification of novel aspects of mineralocorticoid signaling. Nature Publishing Group 2016-11-29 /pmc/articles/PMC5126551/ /pubmed/27897263 http://dx.doi.org/10.1038/srep37991 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Sakamoto, Tatsuya
Yoshiki, Madoka
Takahashi, Hideya
Yoshida, Masayuki
Ogino, Yukiko
Ikeuchi, Toshitaka
Nakamachi, Tomoya
Konno, Norifumi
Matsuda, Kouhei
Sakamoto, Hirotaka
Principal function of mineralocorticoid signaling suggested by constitutive knockout of the mineralocorticoid receptor in medaka fish
title Principal function of mineralocorticoid signaling suggested by constitutive knockout of the mineralocorticoid receptor in medaka fish
title_full Principal function of mineralocorticoid signaling suggested by constitutive knockout of the mineralocorticoid receptor in medaka fish
title_fullStr Principal function of mineralocorticoid signaling suggested by constitutive knockout of the mineralocorticoid receptor in medaka fish
title_full_unstemmed Principal function of mineralocorticoid signaling suggested by constitutive knockout of the mineralocorticoid receptor in medaka fish
title_short Principal function of mineralocorticoid signaling suggested by constitutive knockout of the mineralocorticoid receptor in medaka fish
title_sort principal function of mineralocorticoid signaling suggested by constitutive knockout of the mineralocorticoid receptor in medaka fish
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5126551/
https://www.ncbi.nlm.nih.gov/pubmed/27897263
http://dx.doi.org/10.1038/srep37991
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