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Comprehensive investigation of tobacco leaves during natural early senescence via multi-platform metabolomics analyses

Senescence is the final stage of leaf growth and development. Many different physiological activities occur during this process. A comprehensive metabolomics analysis of tobacco middle leaves at 5 different developmental stages was implemented through multi-platform methods based on liquid chromatog...

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Autores principales: Li, Lili, Zhao, Jieyu, Zhao, Yanni, Lu, Xin, Zhou, Zhihui, Zhao, Chunxia, Xu, Guowang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5126694/
https://www.ncbi.nlm.nih.gov/pubmed/27897248
http://dx.doi.org/10.1038/srep37976
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author Li, Lili
Zhao, Jieyu
Zhao, Yanni
Lu, Xin
Zhou, Zhihui
Zhao, Chunxia
Xu, Guowang
author_facet Li, Lili
Zhao, Jieyu
Zhao, Yanni
Lu, Xin
Zhou, Zhihui
Zhao, Chunxia
Xu, Guowang
author_sort Li, Lili
collection PubMed
description Senescence is the final stage of leaf growth and development. Many different physiological activities occur during this process. A comprehensive metabolomics analysis of tobacco middle leaves at 5 different developmental stages was implemented through multi-platform methods based on liquid chromatography, capillary electrophoresis and gas chromatography coupled with mass spectrometry. In total, 412 metabolites were identified, including pigments, sterols, lipids, amino acids, polyamines, sugars and secondary metabolites. Dramatic metabolic changes were observed. Firstly, membrane degradation and chlorophyll down-regulation occurred after the 50% flower bud stage. Levels of major membrane lipids decreased, including those of the glycolipids in chloroplast thylakoids and phospholipids in membrane envelopes. Clear decreases in free sterols and acylated sterol glucosides were detected along with the accumulation of sterol esters. The accumulation of alkaloids was found. The amino acid levels were significantly decreased, particularly those of N-rich amino acids (glutamine and asparagine), thus reflecting N translocation. Subsequently, the antioxidant system was activated. Sugar alcohols and polyphenols accumulated when the lower leaves turned yellow. These results comprehensively revealed the metabolic changes that occur during tobacco leaf development and senescence under natural conditions.
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spelling pubmed-51266942016-12-09 Comprehensive investigation of tobacco leaves during natural early senescence via multi-platform metabolomics analyses Li, Lili Zhao, Jieyu Zhao, Yanni Lu, Xin Zhou, Zhihui Zhao, Chunxia Xu, Guowang Sci Rep Article Senescence is the final stage of leaf growth and development. Many different physiological activities occur during this process. A comprehensive metabolomics analysis of tobacco middle leaves at 5 different developmental stages was implemented through multi-platform methods based on liquid chromatography, capillary electrophoresis and gas chromatography coupled with mass spectrometry. In total, 412 metabolites were identified, including pigments, sterols, lipids, amino acids, polyamines, sugars and secondary metabolites. Dramatic metabolic changes were observed. Firstly, membrane degradation and chlorophyll down-regulation occurred after the 50% flower bud stage. Levels of major membrane lipids decreased, including those of the glycolipids in chloroplast thylakoids and phospholipids in membrane envelopes. Clear decreases in free sterols and acylated sterol glucosides were detected along with the accumulation of sterol esters. The accumulation of alkaloids was found. The amino acid levels were significantly decreased, particularly those of N-rich amino acids (glutamine and asparagine), thus reflecting N translocation. Subsequently, the antioxidant system was activated. Sugar alcohols and polyphenols accumulated when the lower leaves turned yellow. These results comprehensively revealed the metabolic changes that occur during tobacco leaf development and senescence under natural conditions. Nature Publishing Group 2016-11-29 /pmc/articles/PMC5126694/ /pubmed/27897248 http://dx.doi.org/10.1038/srep37976 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Li, Lili
Zhao, Jieyu
Zhao, Yanni
Lu, Xin
Zhou, Zhihui
Zhao, Chunxia
Xu, Guowang
Comprehensive investigation of tobacco leaves during natural early senescence via multi-platform metabolomics analyses
title Comprehensive investigation of tobacco leaves during natural early senescence via multi-platform metabolomics analyses
title_full Comprehensive investigation of tobacco leaves during natural early senescence via multi-platform metabolomics analyses
title_fullStr Comprehensive investigation of tobacco leaves during natural early senescence via multi-platform metabolomics analyses
title_full_unstemmed Comprehensive investigation of tobacco leaves during natural early senescence via multi-platform metabolomics analyses
title_short Comprehensive investigation of tobacco leaves during natural early senescence via multi-platform metabolomics analyses
title_sort comprehensive investigation of tobacco leaves during natural early senescence via multi-platform metabolomics analyses
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5126694/
https://www.ncbi.nlm.nih.gov/pubmed/27897248
http://dx.doi.org/10.1038/srep37976
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