Gene cassette knock-in in mammalian cells and zygotes by enhanced MMEJ

BACKGROUND: Although CRISPR/Cas enables one-step gene cassette knock-in, assembling targeting vectors containing long homology arms is a laborious process for high-throughput knock-in. We recently developed the CRISPR/Cas-based precise integration into the target chromosome (PITCh) system for a gene...

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Detalles Bibliográficos
Autores principales: Aida, Tomomi, Nakade, Shota, Sakuma, Tetsushi, Izu, Yayoi, Oishi, Ayu, Mochida, Keiji, Ishikubo, Harumi, Usami, Takako, Aizawa, Hidenori, Yamamoto, Takashi, Tanaka, Kohichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5126809/
https://www.ncbi.nlm.nih.gov/pubmed/27894274
http://dx.doi.org/10.1186/s12864-016-3331-9

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