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Anti-inflammatory and burn injury wound healing properties of the shell of Haliotis diversicolor

BACKGROUND: The shell of Haliotis diversicolor, or shijueming (SJM), is a type of traditional Chinese medicine. The SJM has appeared in historical records as early as the third and fourth centuries. Historical records have revealed that SJM had mainly been used to treat eye diseases. After the Qing...

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Autores principales: Chen, Zhi-Cheng, Wu, Shing-Yi Sean, Su, Wei-Yang, Lin, Yuan-Chuan, Lee, Yi-Hsin, Wu, Wei-Hao, Chen, Chun-Hong, Wen, Zhi-Hong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5126830/
https://www.ncbi.nlm.nih.gov/pubmed/27894302
http://dx.doi.org/10.1186/s12906-016-1473-6
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author Chen, Zhi-Cheng
Wu, Shing-Yi Sean
Su, Wei-Yang
Lin, Yuan-Chuan
Lee, Yi-Hsin
Wu, Wei-Hao
Chen, Chun-Hong
Wen, Zhi-Hong
author_facet Chen, Zhi-Cheng
Wu, Shing-Yi Sean
Su, Wei-Yang
Lin, Yuan-Chuan
Lee, Yi-Hsin
Wu, Wei-Hao
Chen, Chun-Hong
Wen, Zhi-Hong
author_sort Chen, Zhi-Cheng
collection PubMed
description BACKGROUND: The shell of Haliotis diversicolor, or shijueming (SJM), is a type of traditional Chinese medicine. The SJM has appeared in historical records as early as the third and fourth centuries. Historical records have revealed that SJM had mainly been used to treat eye diseases. After the Qing Dynasty (1757), records had emerged, detailing the use of SJM for treating skin injuries, particularly for treating poorly managed ulcers or traumatic wounds. Furthermore, in our anti-inflammation-screening system, SJM significantly inhibited the expression of pro-inflammatory proteins. Previous studies have yet to adopt an animal model to verify the phenomenon and described in the historical records regarding the efficacy of SJM in promoting wound healing. Besides, the mechanism of wound healing effect of SJM is also not clear. METHODS: This study applied in vitro and in vivo models, tissue section analysis, and western blotting to evaluate the effect of SJM on wound healing. The RAW 264.7 cells were used in anti-inflammatory activity assay and phagocytic assay. Male Wistar rats were used to evaluate the effect of SJM on burn injury healing. A copper block (2 × 2 cm, 150 g) preheated to 165 °C in a dry bath was used to contact the skin area for 10 s, thus creating a full-thickness burn injury. The results were analyzed by hematoxylin and eosin staining, picrosirius red staining and Western blotting. RESULTS: The results revealed that in the in vitro model, the presence of SJM decreased the inducible nitric oxide synthase (iNOS) expression and enhanced the functions of macrophages. The results of the rat burn injury model revealed that SJM decreased neutrophil infiltration, promoted wound healing, thus increasing the collagen I content and promoting the expression of transforming growth factor-beta 1 (TGF-β1) protein. We speculate that the effect and mechanism of SJM on promoting wound healing is related to macrophage activation. In the inflammation phase, SJM alleviates inflammation by inhibiting iNOS expression and removing neutrophils through phagocytosis. Furthermore, SJM induces the secretion of TGF-β1, converting collagen during the tissue remodeling phase. CONCLUSIONS: According to our review of relevant literature, this is the first study that applied an evidence-based method to verify that SJM alleviates inflammation, enhances phagocytosis, and triggers wound healing after burn injury. The study findings reveal that SJM provides a promising therapeutic option for treating burn injury.
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spelling pubmed-51268302016-12-08 Anti-inflammatory and burn injury wound healing properties of the shell of Haliotis diversicolor Chen, Zhi-Cheng Wu, Shing-Yi Sean Su, Wei-Yang Lin, Yuan-Chuan Lee, Yi-Hsin Wu, Wei-Hao Chen, Chun-Hong Wen, Zhi-Hong BMC Complement Altern Med Research Article BACKGROUND: The shell of Haliotis diversicolor, or shijueming (SJM), is a type of traditional Chinese medicine. The SJM has appeared in historical records as early as the third and fourth centuries. Historical records have revealed that SJM had mainly been used to treat eye diseases. After the Qing Dynasty (1757), records had emerged, detailing the use of SJM for treating skin injuries, particularly for treating poorly managed ulcers or traumatic wounds. Furthermore, in our anti-inflammation-screening system, SJM significantly inhibited the expression of pro-inflammatory proteins. Previous studies have yet to adopt an animal model to verify the phenomenon and described in the historical records regarding the efficacy of SJM in promoting wound healing. Besides, the mechanism of wound healing effect of SJM is also not clear. METHODS: This study applied in vitro and in vivo models, tissue section analysis, and western blotting to evaluate the effect of SJM on wound healing. The RAW 264.7 cells were used in anti-inflammatory activity assay and phagocytic assay. Male Wistar rats were used to evaluate the effect of SJM on burn injury healing. A copper block (2 × 2 cm, 150 g) preheated to 165 °C in a dry bath was used to contact the skin area for 10 s, thus creating a full-thickness burn injury. The results were analyzed by hematoxylin and eosin staining, picrosirius red staining and Western blotting. RESULTS: The results revealed that in the in vitro model, the presence of SJM decreased the inducible nitric oxide synthase (iNOS) expression and enhanced the functions of macrophages. The results of the rat burn injury model revealed that SJM decreased neutrophil infiltration, promoted wound healing, thus increasing the collagen I content and promoting the expression of transforming growth factor-beta 1 (TGF-β1) protein. We speculate that the effect and mechanism of SJM on promoting wound healing is related to macrophage activation. In the inflammation phase, SJM alleviates inflammation by inhibiting iNOS expression and removing neutrophils through phagocytosis. Furthermore, SJM induces the secretion of TGF-β1, converting collagen during the tissue remodeling phase. CONCLUSIONS: According to our review of relevant literature, this is the first study that applied an evidence-based method to verify that SJM alleviates inflammation, enhances phagocytosis, and triggers wound healing after burn injury. The study findings reveal that SJM provides a promising therapeutic option for treating burn injury. BioMed Central 2016-11-28 /pmc/articles/PMC5126830/ /pubmed/27894302 http://dx.doi.org/10.1186/s12906-016-1473-6 Text en © The Author(s). 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Chen, Zhi-Cheng
Wu, Shing-Yi Sean
Su, Wei-Yang
Lin, Yuan-Chuan
Lee, Yi-Hsin
Wu, Wei-Hao
Chen, Chun-Hong
Wen, Zhi-Hong
Anti-inflammatory and burn injury wound healing properties of the shell of Haliotis diversicolor
title Anti-inflammatory and burn injury wound healing properties of the shell of Haliotis diversicolor
title_full Anti-inflammatory and burn injury wound healing properties of the shell of Haliotis diversicolor
title_fullStr Anti-inflammatory and burn injury wound healing properties of the shell of Haliotis diversicolor
title_full_unstemmed Anti-inflammatory and burn injury wound healing properties of the shell of Haliotis diversicolor
title_short Anti-inflammatory and burn injury wound healing properties of the shell of Haliotis diversicolor
title_sort anti-inflammatory and burn injury wound healing properties of the shell of haliotis diversicolor
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5126830/
https://www.ncbi.nlm.nih.gov/pubmed/27894302
http://dx.doi.org/10.1186/s12906-016-1473-6
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