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Cedratvirus, a Double-Cork Structured Giant Virus, is a Distant Relative of Pithoviruses
Most viruses are known for the ability to cause symptomatic diseases in humans and other animals. The discovery of Acanthamoeba polyphaga mimivirus and other giant amoebal viruses revealed a considerable and previously unknown area of uncharacterized viral particles. Giant viruses have been isolated...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5127014/ https://www.ncbi.nlm.nih.gov/pubmed/27827884 http://dx.doi.org/10.3390/v8110300 |
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author | Andreani, Julien Aherfi, Sarah Bou Khalil, Jacques Yaacoub Di Pinto, Fabrizio Bitam, Idir Raoult, Didier Colson, Philippe La Scola, Bernard |
author_facet | Andreani, Julien Aherfi, Sarah Bou Khalil, Jacques Yaacoub Di Pinto, Fabrizio Bitam, Idir Raoult, Didier Colson, Philippe La Scola, Bernard |
author_sort | Andreani, Julien |
collection | PubMed |
description | Most viruses are known for the ability to cause symptomatic diseases in humans and other animals. The discovery of Acanthamoeba polyphaga mimivirus and other giant amoebal viruses revealed a considerable and previously unknown area of uncharacterized viral particles. Giant viruses have been isolated from various environmental samples collected from very distant geographic places, revealing a ubiquitous distribution. Their morphological and genomic features are fundamental elements for classifying them. Herein, we report the isolation and draft genome of Cedratvirus, a new amoebal giant virus isolated in Acanthamoeba castellanii, from an Algerian environmental sample. The viral particles are ovoid-shaped, resembling Pithovirus sibericum, but differing notably in the presence of two corks at each extremity of the virion. The draft genome of Cedratvirus—589,068 base pairs in length—is a close relative of the two previously described pithoviruses, sharing 104 and 113 genes with P. sibericum and Pithovirus massiliensis genomes, respectively. Interestingly, analysis of these viruses’ core genome reveals that only 21% of Cedratvirus genes are involved in best reciprocal hits with the two pithoviruses. Phylogeny reconstructions and comparative genomics indicate that Cedratvirus is most closely related to pithoviruses, and questions their membership in an enlarged putative Pithoviridae family. |
format | Online Article Text |
id | pubmed-5127014 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-51270142016-12-02 Cedratvirus, a Double-Cork Structured Giant Virus, is a Distant Relative of Pithoviruses Andreani, Julien Aherfi, Sarah Bou Khalil, Jacques Yaacoub Di Pinto, Fabrizio Bitam, Idir Raoult, Didier Colson, Philippe La Scola, Bernard Viruses Article Most viruses are known for the ability to cause symptomatic diseases in humans and other animals. The discovery of Acanthamoeba polyphaga mimivirus and other giant amoebal viruses revealed a considerable and previously unknown area of uncharacterized viral particles. Giant viruses have been isolated from various environmental samples collected from very distant geographic places, revealing a ubiquitous distribution. Their morphological and genomic features are fundamental elements for classifying them. Herein, we report the isolation and draft genome of Cedratvirus, a new amoebal giant virus isolated in Acanthamoeba castellanii, from an Algerian environmental sample. The viral particles are ovoid-shaped, resembling Pithovirus sibericum, but differing notably in the presence of two corks at each extremity of the virion. The draft genome of Cedratvirus—589,068 base pairs in length—is a close relative of the two previously described pithoviruses, sharing 104 and 113 genes with P. sibericum and Pithovirus massiliensis genomes, respectively. Interestingly, analysis of these viruses’ core genome reveals that only 21% of Cedratvirus genes are involved in best reciprocal hits with the two pithoviruses. Phylogeny reconstructions and comparative genomics indicate that Cedratvirus is most closely related to pithoviruses, and questions their membership in an enlarged putative Pithoviridae family. MDPI 2016-11-03 /pmc/articles/PMC5127014/ /pubmed/27827884 http://dx.doi.org/10.3390/v8110300 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Andreani, Julien Aherfi, Sarah Bou Khalil, Jacques Yaacoub Di Pinto, Fabrizio Bitam, Idir Raoult, Didier Colson, Philippe La Scola, Bernard Cedratvirus, a Double-Cork Structured Giant Virus, is a Distant Relative of Pithoviruses |
title | Cedratvirus, a Double-Cork Structured Giant Virus, is a Distant Relative of Pithoviruses |
title_full | Cedratvirus, a Double-Cork Structured Giant Virus, is a Distant Relative of Pithoviruses |
title_fullStr | Cedratvirus, a Double-Cork Structured Giant Virus, is a Distant Relative of Pithoviruses |
title_full_unstemmed | Cedratvirus, a Double-Cork Structured Giant Virus, is a Distant Relative of Pithoviruses |
title_short | Cedratvirus, a Double-Cork Structured Giant Virus, is a Distant Relative of Pithoviruses |
title_sort | cedratvirus, a double-cork structured giant virus, is a distant relative of pithoviruses |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5127014/ https://www.ncbi.nlm.nih.gov/pubmed/27827884 http://dx.doi.org/10.3390/v8110300 |
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