Cargando…

Cedratvirus, a Double-Cork Structured Giant Virus, is a Distant Relative of Pithoviruses

Most viruses are known for the ability to cause symptomatic diseases in humans and other animals. The discovery of Acanthamoeba polyphaga mimivirus and other giant amoebal viruses revealed a considerable and previously unknown area of uncharacterized viral particles. Giant viruses have been isolated...

Descripción completa

Detalles Bibliográficos
Autores principales: Andreani, Julien, Aherfi, Sarah, Bou Khalil, Jacques Yaacoub, Di Pinto, Fabrizio, Bitam, Idir, Raoult, Didier, Colson, Philippe, La Scola, Bernard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5127014/
https://www.ncbi.nlm.nih.gov/pubmed/27827884
http://dx.doi.org/10.3390/v8110300
_version_ 1782470195365806080
author Andreani, Julien
Aherfi, Sarah
Bou Khalil, Jacques Yaacoub
Di Pinto, Fabrizio
Bitam, Idir
Raoult, Didier
Colson, Philippe
La Scola, Bernard
author_facet Andreani, Julien
Aherfi, Sarah
Bou Khalil, Jacques Yaacoub
Di Pinto, Fabrizio
Bitam, Idir
Raoult, Didier
Colson, Philippe
La Scola, Bernard
author_sort Andreani, Julien
collection PubMed
description Most viruses are known for the ability to cause symptomatic diseases in humans and other animals. The discovery of Acanthamoeba polyphaga mimivirus and other giant amoebal viruses revealed a considerable and previously unknown area of uncharacterized viral particles. Giant viruses have been isolated from various environmental samples collected from very distant geographic places, revealing a ubiquitous distribution. Their morphological and genomic features are fundamental elements for classifying them. Herein, we report the isolation and draft genome of Cedratvirus, a new amoebal giant virus isolated in Acanthamoeba castellanii, from an Algerian environmental sample. The viral particles are ovoid-shaped, resembling Pithovirus sibericum, but differing notably in the presence of two corks at each extremity of the virion. The draft genome of Cedratvirus—589,068 base pairs in length—is a close relative of the two previously described pithoviruses, sharing 104 and 113 genes with P. sibericum and Pithovirus massiliensis genomes, respectively. Interestingly, analysis of these viruses’ core genome reveals that only 21% of Cedratvirus genes are involved in best reciprocal hits with the two pithoviruses. Phylogeny reconstructions and comparative genomics indicate that Cedratvirus is most closely related to pithoviruses, and questions their membership in an enlarged putative Pithoviridae family.
format Online
Article
Text
id pubmed-5127014
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-51270142016-12-02 Cedratvirus, a Double-Cork Structured Giant Virus, is a Distant Relative of Pithoviruses Andreani, Julien Aherfi, Sarah Bou Khalil, Jacques Yaacoub Di Pinto, Fabrizio Bitam, Idir Raoult, Didier Colson, Philippe La Scola, Bernard Viruses Article Most viruses are known for the ability to cause symptomatic diseases in humans and other animals. The discovery of Acanthamoeba polyphaga mimivirus and other giant amoebal viruses revealed a considerable and previously unknown area of uncharacterized viral particles. Giant viruses have been isolated from various environmental samples collected from very distant geographic places, revealing a ubiquitous distribution. Their morphological and genomic features are fundamental elements for classifying them. Herein, we report the isolation and draft genome of Cedratvirus, a new amoebal giant virus isolated in Acanthamoeba castellanii, from an Algerian environmental sample. The viral particles are ovoid-shaped, resembling Pithovirus sibericum, but differing notably in the presence of two corks at each extremity of the virion. The draft genome of Cedratvirus—589,068 base pairs in length—is a close relative of the two previously described pithoviruses, sharing 104 and 113 genes with P. sibericum and Pithovirus massiliensis genomes, respectively. Interestingly, analysis of these viruses’ core genome reveals that only 21% of Cedratvirus genes are involved in best reciprocal hits with the two pithoviruses. Phylogeny reconstructions and comparative genomics indicate that Cedratvirus is most closely related to pithoviruses, and questions their membership in an enlarged putative Pithoviridae family. MDPI 2016-11-03 /pmc/articles/PMC5127014/ /pubmed/27827884 http://dx.doi.org/10.3390/v8110300 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Andreani, Julien
Aherfi, Sarah
Bou Khalil, Jacques Yaacoub
Di Pinto, Fabrizio
Bitam, Idir
Raoult, Didier
Colson, Philippe
La Scola, Bernard
Cedratvirus, a Double-Cork Structured Giant Virus, is a Distant Relative of Pithoviruses
title Cedratvirus, a Double-Cork Structured Giant Virus, is a Distant Relative of Pithoviruses
title_full Cedratvirus, a Double-Cork Structured Giant Virus, is a Distant Relative of Pithoviruses
title_fullStr Cedratvirus, a Double-Cork Structured Giant Virus, is a Distant Relative of Pithoviruses
title_full_unstemmed Cedratvirus, a Double-Cork Structured Giant Virus, is a Distant Relative of Pithoviruses
title_short Cedratvirus, a Double-Cork Structured Giant Virus, is a Distant Relative of Pithoviruses
title_sort cedratvirus, a double-cork structured giant virus, is a distant relative of pithoviruses
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5127014/
https://www.ncbi.nlm.nih.gov/pubmed/27827884
http://dx.doi.org/10.3390/v8110300
work_keys_str_mv AT andreanijulien cedratvirusadoublecorkstructuredgiantvirusisadistantrelativeofpithoviruses
AT aherfisarah cedratvirusadoublecorkstructuredgiantvirusisadistantrelativeofpithoviruses
AT boukhaliljacquesyaacoub cedratvirusadoublecorkstructuredgiantvirusisadistantrelativeofpithoviruses
AT dipintofabrizio cedratvirusadoublecorkstructuredgiantvirusisadistantrelativeofpithoviruses
AT bitamidir cedratvirusadoublecorkstructuredgiantvirusisadistantrelativeofpithoviruses
AT raoultdidier cedratvirusadoublecorkstructuredgiantvirusisadistantrelativeofpithoviruses
AT colsonphilippe cedratvirusadoublecorkstructuredgiantvirusisadistantrelativeofpithoviruses
AT lascolabernard cedratvirusadoublecorkstructuredgiantvirusisadistantrelativeofpithoviruses