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Curcumin Prevents Aflatoxin B(1) Hepatoxicity by Inhibition of Cytochrome P450 Isozymes in Chick Liver
This study was designed to establish if Curcumin (CM) alleviates Aflatoxin B(1) (AFB(1))-induced hepatotoxic effects and to determine whether alteration of the expression of cytochrome P450 (CYP450) isozymes is involved in the regulation of these effects in chick liver. One-day-old male broilers (n...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5127124/ https://www.ncbi.nlm.nih.gov/pubmed/27834912 http://dx.doi.org/10.3390/toxins8110327 |
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author | Zhang, Ni-Ya Qi, Ming Zhao, Ling Zhu, Ming-Kun Guo, Jiao Liu, Jie Gu, Chang-Qin Rajput, Shahid Ali Krumm, Christopher Steven Qi, De-Sheng Sun, Lv-Hui |
author_facet | Zhang, Ni-Ya Qi, Ming Zhao, Ling Zhu, Ming-Kun Guo, Jiao Liu, Jie Gu, Chang-Qin Rajput, Shahid Ali Krumm, Christopher Steven Qi, De-Sheng Sun, Lv-Hui |
author_sort | Zhang, Ni-Ya |
collection | PubMed |
description | This study was designed to establish if Curcumin (CM) alleviates Aflatoxin B(1) (AFB(1))-induced hepatotoxic effects and to determine whether alteration of the expression of cytochrome P450 (CYP450) isozymes is involved in the regulation of these effects in chick liver. One-day-old male broilers (n = 120) were divided into four groups and used in a two by two factorial trial in which the main factors included supplementing AFB(1) (< 5 vs. 100 μg/kg) and CM (0 vs. 150 mg/kg) in a corn/soybean-based diet. Administration of AFB(1) induced liver injury, significantly decreasing albumin and total protein concentrations and increasing alanine aminotransferase and aspartate aminotransferase activities in serum, and induced hepatic histological lesions at week 2. AFB(1) also significantly decreased hepatic glutathione peroxidase, catalase, and glutathione levels, while increasing malondialdehyde, 8-hydroxydeoxyguanosine, and exo-AFB(1)-8,9-epoxide (AFBO)-DNA concentrations. In addition, the mRNA and/or activity of enzymes responsible for the bioactivation of AFB(1) into AFBO—including CYP1A1, CYP1A2, CYP2A6, and CYP3A4—were significantly induced in liver microsomes after 2-week exposure to AFB(1). These alterations induced by AFB(1) were prevented by CM supplementation. Conclusively, dietary CM protected chicks from AFB(1)-induced liver injury, potentially through the synergistic actions of increased antioxidant capacities and inhibition of the pivotal CYP450 isozyme-mediated activation of AFB(1) to toxic AFBO. |
format | Online Article Text |
id | pubmed-5127124 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-51271242016-12-02 Curcumin Prevents Aflatoxin B(1) Hepatoxicity by Inhibition of Cytochrome P450 Isozymes in Chick Liver Zhang, Ni-Ya Qi, Ming Zhao, Ling Zhu, Ming-Kun Guo, Jiao Liu, Jie Gu, Chang-Qin Rajput, Shahid Ali Krumm, Christopher Steven Qi, De-Sheng Sun, Lv-Hui Toxins (Basel) Article This study was designed to establish if Curcumin (CM) alleviates Aflatoxin B(1) (AFB(1))-induced hepatotoxic effects and to determine whether alteration of the expression of cytochrome P450 (CYP450) isozymes is involved in the regulation of these effects in chick liver. One-day-old male broilers (n = 120) were divided into four groups and used in a two by two factorial trial in which the main factors included supplementing AFB(1) (< 5 vs. 100 μg/kg) and CM (0 vs. 150 mg/kg) in a corn/soybean-based diet. Administration of AFB(1) induced liver injury, significantly decreasing albumin and total protein concentrations and increasing alanine aminotransferase and aspartate aminotransferase activities in serum, and induced hepatic histological lesions at week 2. AFB(1) also significantly decreased hepatic glutathione peroxidase, catalase, and glutathione levels, while increasing malondialdehyde, 8-hydroxydeoxyguanosine, and exo-AFB(1)-8,9-epoxide (AFBO)-DNA concentrations. In addition, the mRNA and/or activity of enzymes responsible for the bioactivation of AFB(1) into AFBO—including CYP1A1, CYP1A2, CYP2A6, and CYP3A4—were significantly induced in liver microsomes after 2-week exposure to AFB(1). These alterations induced by AFB(1) were prevented by CM supplementation. Conclusively, dietary CM protected chicks from AFB(1)-induced liver injury, potentially through the synergistic actions of increased antioxidant capacities and inhibition of the pivotal CYP450 isozyme-mediated activation of AFB(1) to toxic AFBO. MDPI 2016-11-10 /pmc/articles/PMC5127124/ /pubmed/27834912 http://dx.doi.org/10.3390/toxins8110327 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zhang, Ni-Ya Qi, Ming Zhao, Ling Zhu, Ming-Kun Guo, Jiao Liu, Jie Gu, Chang-Qin Rajput, Shahid Ali Krumm, Christopher Steven Qi, De-Sheng Sun, Lv-Hui Curcumin Prevents Aflatoxin B(1) Hepatoxicity by Inhibition of Cytochrome P450 Isozymes in Chick Liver |
title | Curcumin Prevents Aflatoxin B(1) Hepatoxicity by Inhibition of Cytochrome P450 Isozymes in Chick Liver |
title_full | Curcumin Prevents Aflatoxin B(1) Hepatoxicity by Inhibition of Cytochrome P450 Isozymes in Chick Liver |
title_fullStr | Curcumin Prevents Aflatoxin B(1) Hepatoxicity by Inhibition of Cytochrome P450 Isozymes in Chick Liver |
title_full_unstemmed | Curcumin Prevents Aflatoxin B(1) Hepatoxicity by Inhibition of Cytochrome P450 Isozymes in Chick Liver |
title_short | Curcumin Prevents Aflatoxin B(1) Hepatoxicity by Inhibition of Cytochrome P450 Isozymes in Chick Liver |
title_sort | curcumin prevents aflatoxin b(1) hepatoxicity by inhibition of cytochrome p450 isozymes in chick liver |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5127124/ https://www.ncbi.nlm.nih.gov/pubmed/27834912 http://dx.doi.org/10.3390/toxins8110327 |
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