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Programmed necrosis - a new mechanism of steroidogenic luteal cell death and elimination during luteolysis in cows
Programmed necrosis (necroptosis) is an alternative form of programmed cell death that is regulated by receptor-interacting protein kinase (RIPK) 1 and 3-dependent, but is a caspase (CASP)-independent pathway. In the present study, to determine if necroptosis participates in bovine structural luteol...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5128806/ https://www.ncbi.nlm.nih.gov/pubmed/27901113 http://dx.doi.org/10.1038/srep38211 |
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author | Hojo, Takuo Siemieniuch, Marta J. Lukasik, Karolina Piotrowska-Tomala, Katarzyna K. Jonczyk, Agnieszka W. Okuda, Kiyoshi Skarzynski, Dariusz J. |
author_facet | Hojo, Takuo Siemieniuch, Marta J. Lukasik, Karolina Piotrowska-Tomala, Katarzyna K. Jonczyk, Agnieszka W. Okuda, Kiyoshi Skarzynski, Dariusz J. |
author_sort | Hojo, Takuo |
collection | PubMed |
description | Programmed necrosis (necroptosis) is an alternative form of programmed cell death that is regulated by receptor-interacting protein kinase (RIPK) 1 and 3-dependent, but is a caspase (CASP)-independent pathway. In the present study, to determine if necroptosis participates in bovine structural luteolysis, we investigated RIPK1 and RIPK3 expression throughout the estrous cycle, during prostaglandin F2α (PGF)-induced luteolysis in the bovine corpus luteum (CL), and in cultured luteal steroidogenic cells (LSCs) after treatment with selected luteolytic factors. In addition, effects of a RIPK1 inhibitor (necrostatin-1, Nec-1; 50 μM) on cell viability, progesterone secretion, apoptosis related factors and RIPKs expression, were evaluated. Expression of RIPK1 and RIPK3 increased in the CL tissue during both spontaneous and PGF-induced luteolysis (P < 0.05). In cultured LSCs, tumor necrosis factor α (TNF; 2.3 nM) in combination with interferon γ (IFNG; 2.5 nM) up-regulated RIPK1 mRNA and protein expression (P < 0.05). TNF + IFNG also up-regulated RIPK3 mRNA expression (P < 0.05), but not RIPK3 protein. Although Nec-1 prevented TNF + IFNG-induced cell death (P < 0.05), it did not affect CASP3 and CASP8 expression. Nec-1 decreased both RIPK1 and RIPK3 protein expression (P < 0.05). These findings suggest that RIPKs-dependent necroptosis is a potent mechanism responsible for bovine structural luteolysis induced by pro-inflammatory cytokines. |
format | Online Article Text |
id | pubmed-5128806 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-51288062016-12-09 Programmed necrosis - a new mechanism of steroidogenic luteal cell death and elimination during luteolysis in cows Hojo, Takuo Siemieniuch, Marta J. Lukasik, Karolina Piotrowska-Tomala, Katarzyna K. Jonczyk, Agnieszka W. Okuda, Kiyoshi Skarzynski, Dariusz J. Sci Rep Article Programmed necrosis (necroptosis) is an alternative form of programmed cell death that is regulated by receptor-interacting protein kinase (RIPK) 1 and 3-dependent, but is a caspase (CASP)-independent pathway. In the present study, to determine if necroptosis participates in bovine structural luteolysis, we investigated RIPK1 and RIPK3 expression throughout the estrous cycle, during prostaglandin F2α (PGF)-induced luteolysis in the bovine corpus luteum (CL), and in cultured luteal steroidogenic cells (LSCs) after treatment with selected luteolytic factors. In addition, effects of a RIPK1 inhibitor (necrostatin-1, Nec-1; 50 μM) on cell viability, progesterone secretion, apoptosis related factors and RIPKs expression, were evaluated. Expression of RIPK1 and RIPK3 increased in the CL tissue during both spontaneous and PGF-induced luteolysis (P < 0.05). In cultured LSCs, tumor necrosis factor α (TNF; 2.3 nM) in combination with interferon γ (IFNG; 2.5 nM) up-regulated RIPK1 mRNA and protein expression (P < 0.05). TNF + IFNG also up-regulated RIPK3 mRNA expression (P < 0.05), but not RIPK3 protein. Although Nec-1 prevented TNF + IFNG-induced cell death (P < 0.05), it did not affect CASP3 and CASP8 expression. Nec-1 decreased both RIPK1 and RIPK3 protein expression (P < 0.05). These findings suggest that RIPKs-dependent necroptosis is a potent mechanism responsible for bovine structural luteolysis induced by pro-inflammatory cytokines. Nature Publishing Group 2016-11-30 /pmc/articles/PMC5128806/ /pubmed/27901113 http://dx.doi.org/10.1038/srep38211 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Hojo, Takuo Siemieniuch, Marta J. Lukasik, Karolina Piotrowska-Tomala, Katarzyna K. Jonczyk, Agnieszka W. Okuda, Kiyoshi Skarzynski, Dariusz J. Programmed necrosis - a new mechanism of steroidogenic luteal cell death and elimination during luteolysis in cows |
title | Programmed necrosis - a new mechanism of steroidogenic luteal cell death and elimination during luteolysis in cows |
title_full | Programmed necrosis - a new mechanism of steroidogenic luteal cell death and elimination during luteolysis in cows |
title_fullStr | Programmed necrosis - a new mechanism of steroidogenic luteal cell death and elimination during luteolysis in cows |
title_full_unstemmed | Programmed necrosis - a new mechanism of steroidogenic luteal cell death and elimination during luteolysis in cows |
title_short | Programmed necrosis - a new mechanism of steroidogenic luteal cell death and elimination during luteolysis in cows |
title_sort | programmed necrosis - a new mechanism of steroidogenic luteal cell death and elimination during luteolysis in cows |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5128806/ https://www.ncbi.nlm.nih.gov/pubmed/27901113 http://dx.doi.org/10.1038/srep38211 |
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