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Effects of Di-(2-ethylhexyl) Phthalate on Lipid Metabolism by the JAK/STAT Pathway in Rats
The most widely used plasticizer, di-(2-ethylhexyl) phthalate (DEHP), is known to affect lipid metabolism and adipogenesis. We studied the effects of dietary DEHP exposure on metabolism in rats as well as the role of the JAK/STAT pathway in this process. Eighty rats were exposed to DEHP (0, 5, 50 an...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5129295/ https://www.ncbi.nlm.nih.gov/pubmed/27827939 http://dx.doi.org/10.3390/ijerph13111085 |
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author | Jia, Yiyang Liu, Te Zhou, Liting Zhu, Jian Wu, Juan Sun, Di Xu, Jin Wang, Qi Chen, Huaiji Xu, Feng Zhang, Yuezhu Zhang, Tianrong Liu, Hongbo Ye, Lin |
author_facet | Jia, Yiyang Liu, Te Zhou, Liting Zhu, Jian Wu, Juan Sun, Di Xu, Jin Wang, Qi Chen, Huaiji Xu, Feng Zhang, Yuezhu Zhang, Tianrong Liu, Hongbo Ye, Lin |
author_sort | Jia, Yiyang |
collection | PubMed |
description | The most widely used plasticizer, di-(2-ethylhexyl) phthalate (DEHP), is known to affect lipid metabolism and adipogenesis. We studied the effects of dietary DEHP exposure on metabolism in rats as well as the role of the JAK/STAT pathway in this process. Eighty rats were exposed to DEHP (0, 5, 50 and 500 mg/kg/d) through dietary intake for 4 weeks. We then collected blood samples, liver, and adipose tissues to detect modifications in the levels of serum lipids, leptin, adiponectin and insulin. JAK3, STAT5a and PPARγ expression were detected at both the gene and protein levels. The activation of JAK3 and STAT5a was also detected. The DEHP-exposed rats had increased body weight, serum lipid, insulin, and leptin levels. Moreover, the JAK3/STAT5a pathway was activated in the adipose tissue; however, this pathway was not activated in the liver. The mRNA of SREBP-1c in the liver was increased significantly among each of the groups, in contrast to the levels found in the mature SREBP-1c protein form. Furthermore, the expression of FABP4, Acox and FASn was decreased in the liver, but increased in adipose tissue. Thus, we conclude that exposure to DEHP reduces the hydrolysis of lipid and promotes triglyceride accumulation by oppositely regulating the activation state of JAK/STAT pathway in the liver and adipose tissue, resulting in the disorder of body lipid metabolism and obesity. |
format | Online Article Text |
id | pubmed-5129295 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-51292952016-12-11 Effects of Di-(2-ethylhexyl) Phthalate on Lipid Metabolism by the JAK/STAT Pathway in Rats Jia, Yiyang Liu, Te Zhou, Liting Zhu, Jian Wu, Juan Sun, Di Xu, Jin Wang, Qi Chen, Huaiji Xu, Feng Zhang, Yuezhu Zhang, Tianrong Liu, Hongbo Ye, Lin Int J Environ Res Public Health Article The most widely used plasticizer, di-(2-ethylhexyl) phthalate (DEHP), is known to affect lipid metabolism and adipogenesis. We studied the effects of dietary DEHP exposure on metabolism in rats as well as the role of the JAK/STAT pathway in this process. Eighty rats were exposed to DEHP (0, 5, 50 and 500 mg/kg/d) through dietary intake for 4 weeks. We then collected blood samples, liver, and adipose tissues to detect modifications in the levels of serum lipids, leptin, adiponectin and insulin. JAK3, STAT5a and PPARγ expression were detected at both the gene and protein levels. The activation of JAK3 and STAT5a was also detected. The DEHP-exposed rats had increased body weight, serum lipid, insulin, and leptin levels. Moreover, the JAK3/STAT5a pathway was activated in the adipose tissue; however, this pathway was not activated in the liver. The mRNA of SREBP-1c in the liver was increased significantly among each of the groups, in contrast to the levels found in the mature SREBP-1c protein form. Furthermore, the expression of FABP4, Acox and FASn was decreased in the liver, but increased in adipose tissue. Thus, we conclude that exposure to DEHP reduces the hydrolysis of lipid and promotes triglyceride accumulation by oppositely regulating the activation state of JAK/STAT pathway in the liver and adipose tissue, resulting in the disorder of body lipid metabolism and obesity. MDPI 2016-11-04 2016-11 /pmc/articles/PMC5129295/ /pubmed/27827939 http://dx.doi.org/10.3390/ijerph13111085 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Jia, Yiyang Liu, Te Zhou, Liting Zhu, Jian Wu, Juan Sun, Di Xu, Jin Wang, Qi Chen, Huaiji Xu, Feng Zhang, Yuezhu Zhang, Tianrong Liu, Hongbo Ye, Lin Effects of Di-(2-ethylhexyl) Phthalate on Lipid Metabolism by the JAK/STAT Pathway in Rats |
title | Effects of Di-(2-ethylhexyl) Phthalate on Lipid Metabolism by the JAK/STAT Pathway in Rats |
title_full | Effects of Di-(2-ethylhexyl) Phthalate on Lipid Metabolism by the JAK/STAT Pathway in Rats |
title_fullStr | Effects of Di-(2-ethylhexyl) Phthalate on Lipid Metabolism by the JAK/STAT Pathway in Rats |
title_full_unstemmed | Effects of Di-(2-ethylhexyl) Phthalate on Lipid Metabolism by the JAK/STAT Pathway in Rats |
title_short | Effects of Di-(2-ethylhexyl) Phthalate on Lipid Metabolism by the JAK/STAT Pathway in Rats |
title_sort | effects of di-(2-ethylhexyl) phthalate on lipid metabolism by the jak/stat pathway in rats |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5129295/ https://www.ncbi.nlm.nih.gov/pubmed/27827939 http://dx.doi.org/10.3390/ijerph13111085 |
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