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Live and inactivated vaccine regimens against caecal Salmonella Typhimurium colonisation in laying hens

OBJECTIVE: In Australia, Salmonella serovar Typhimurium (S. Typhimurium) is the predominant zoonotic serovar in humans and is frequently isolated from layer hens. Vaccination against this serovar has been previously shown to be effective in broilers and the aim of this current study was to assess an...

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Autores principales: Groves, PJ, Sharpe, SM, Muir, WI, Pavic, A, Cox, JM
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wiley Publishing Asia Pty Ltd 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5129469/
https://www.ncbi.nlm.nih.gov/pubmed/27671084
http://dx.doi.org/10.1111/avj.12490
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author Groves, PJ
Sharpe, SM
Muir, WI
Pavic, A
Cox, JM
author_facet Groves, PJ
Sharpe, SM
Muir, WI
Pavic, A
Cox, JM
author_sort Groves, PJ
collection PubMed
description OBJECTIVE: In Australia, Salmonella serovar Typhimurium (S. Typhimurium) is the predominant zoonotic serovar in humans and is frequently isolated from layer hens. Vaccination against this serovar has been previously shown to be effective in broilers and the aim of this current study was to assess and determine the best vaccination strategy (live or inactivated) to minimise caecal colonisation by S. Typhimurium. METHODS: A long‐term experiment (56 weeks) was conducted on ISABROWN pullets using a commercial live aroA deleted mutant S. Typhimurium vaccine and an autogenous inactivated multivalent Salmonella vaccine (containing serovars Typhimurium, Infantis, Montevideo and Zanzibar). These vaccines were administered PO or by SC or IM injection, either alone or in combination. Pullets were vaccinated throughout rearing (to 18 weeks of age) and sequentially bled for antibody titre levels. The birds, vaccinated and controls, were challenged orally with a field isolate of S. Typhimurium at different ages, held for 21 days post‐challenge, then euthanased and their caeca cultured for the presence of Salmonella. RESULTS: None of the oral live‐vaccinated groups exhibited lasting protection. When administered twice, the inactivated vaccine gave significant protection at 17 weeks of age and the live vaccine given by SC injection given twice produced significant protection at 17, 25 and 34 weeks. CONCLUSIONS: Vaccination regimens that included parenteral administration of live or inactivated vaccines and thus achieved positive serum antibody levels were able to provide protection against challenge. Hence, vaccination may play a useful role in a management strategy for Salmonella carriage in layer flocks.
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spelling pubmed-51294692016-11-30 Live and inactivated vaccine regimens against caecal Salmonella Typhimurium colonisation in laying hens Groves, PJ Sharpe, SM Muir, WI Pavic, A Cox, JM Aust Vet J Avian OBJECTIVE: In Australia, Salmonella serovar Typhimurium (S. Typhimurium) is the predominant zoonotic serovar in humans and is frequently isolated from layer hens. Vaccination against this serovar has been previously shown to be effective in broilers and the aim of this current study was to assess and determine the best vaccination strategy (live or inactivated) to minimise caecal colonisation by S. Typhimurium. METHODS: A long‐term experiment (56 weeks) was conducted on ISABROWN pullets using a commercial live aroA deleted mutant S. Typhimurium vaccine and an autogenous inactivated multivalent Salmonella vaccine (containing serovars Typhimurium, Infantis, Montevideo and Zanzibar). These vaccines were administered PO or by SC or IM injection, either alone or in combination. Pullets were vaccinated throughout rearing (to 18 weeks of age) and sequentially bled for antibody titre levels. The birds, vaccinated and controls, were challenged orally with a field isolate of S. Typhimurium at different ages, held for 21 days post‐challenge, then euthanased and their caeca cultured for the presence of Salmonella. RESULTS: None of the oral live‐vaccinated groups exhibited lasting protection. When administered twice, the inactivated vaccine gave significant protection at 17 weeks of age and the live vaccine given by SC injection given twice produced significant protection at 17, 25 and 34 weeks. CONCLUSIONS: Vaccination regimens that included parenteral administration of live or inactivated vaccines and thus achieved positive serum antibody levels were able to provide protection against challenge. Hence, vaccination may play a useful role in a management strategy for Salmonella carriage in layer flocks. Wiley Publishing Asia Pty Ltd 2016-09-27 2016-10 /pmc/articles/PMC5129469/ /pubmed/27671084 http://dx.doi.org/10.1111/avj.12490 Text en © 2016 The Authors. Australian Veterinary Journal published by John Wiley & Sons Australia, Ltd on behalf of Australian Veterinary Association. This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs (http://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Avian
Groves, PJ
Sharpe, SM
Muir, WI
Pavic, A
Cox, JM
Live and inactivated vaccine regimens against caecal Salmonella Typhimurium colonisation in laying hens
title Live and inactivated vaccine regimens against caecal Salmonella Typhimurium colonisation in laying hens
title_full Live and inactivated vaccine regimens against caecal Salmonella Typhimurium colonisation in laying hens
title_fullStr Live and inactivated vaccine regimens against caecal Salmonella Typhimurium colonisation in laying hens
title_full_unstemmed Live and inactivated vaccine regimens against caecal Salmonella Typhimurium colonisation in laying hens
title_short Live and inactivated vaccine regimens against caecal Salmonella Typhimurium colonisation in laying hens
title_sort live and inactivated vaccine regimens against caecal salmonella typhimurium colonisation in laying hens
topic Avian
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5129469/
https://www.ncbi.nlm.nih.gov/pubmed/27671084
http://dx.doi.org/10.1111/avj.12490
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