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Apoptosis-Related Gene Expression Profiling in Hematopoietic Cell Fractions of MDS Patients
Although the vast majority of patients with a myelodysplastic syndrome (MDS) suffer from cytopenias, the bone marrow is usually normocellular or hypercellular. Apoptosis of hematopoietic cells in the bone marrow has been implicated in this phenomenon. However, in MDS it remains only partially elucid...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5130187/ https://www.ncbi.nlm.nih.gov/pubmed/27902785 http://dx.doi.org/10.1371/journal.pone.0165582 |
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author | Langemeijer, Saskia MC Mariani, Niccolo Knops, Ruth Gilissen, Christian Woestenenk, Rob de Witte, Theo Huls, Gerwin van der Reijden, Bert A Jansen, Joop H |
author_facet | Langemeijer, Saskia MC Mariani, Niccolo Knops, Ruth Gilissen, Christian Woestenenk, Rob de Witte, Theo Huls, Gerwin van der Reijden, Bert A Jansen, Joop H |
author_sort | Langemeijer, Saskia MC |
collection | PubMed |
description | Although the vast majority of patients with a myelodysplastic syndrome (MDS) suffer from cytopenias, the bone marrow is usually normocellular or hypercellular. Apoptosis of hematopoietic cells in the bone marrow has been implicated in this phenomenon. However, in MDS it remains only partially elucidated which genes are involved in this process and which hematopoietic cells are mainly affected. We employed sensitive real-time PCR technology to study 93 apoptosis-related genes and gene families in sorted immature CD34+ and the differentiating erythroid (CD71+) and monomyeloid (CD13/33+) bone marrow cells. Unsupervised cluster analysis of the expression signature readily distinguished the different cellular bone marrow fractions (CD34+, CD71+ and CD13/33+) from each other, but did not discriminate patients from healthy controls. When individual genes were regarded, several were found to be differentially expressed between patients and controls. Particularly, strong over-expression of BIK (BCL2-interacting killer) was observed in erythroid progenitor cells of low- and high-risk MDS patients (both p = 0.001) and TNFRSF4 (tumor necrosis factor receptor superfamily 4) was down-regulated in immature hematopoietic cells (p = 0.0023) of low-risk MDS patients compared to healthy bone marrow. |
format | Online Article Text |
id | pubmed-5130187 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-51301872016-12-15 Apoptosis-Related Gene Expression Profiling in Hematopoietic Cell Fractions of MDS Patients Langemeijer, Saskia MC Mariani, Niccolo Knops, Ruth Gilissen, Christian Woestenenk, Rob de Witte, Theo Huls, Gerwin van der Reijden, Bert A Jansen, Joop H PLoS One Research Article Although the vast majority of patients with a myelodysplastic syndrome (MDS) suffer from cytopenias, the bone marrow is usually normocellular or hypercellular. Apoptosis of hematopoietic cells in the bone marrow has been implicated in this phenomenon. However, in MDS it remains only partially elucidated which genes are involved in this process and which hematopoietic cells are mainly affected. We employed sensitive real-time PCR technology to study 93 apoptosis-related genes and gene families in sorted immature CD34+ and the differentiating erythroid (CD71+) and monomyeloid (CD13/33+) bone marrow cells. Unsupervised cluster analysis of the expression signature readily distinguished the different cellular bone marrow fractions (CD34+, CD71+ and CD13/33+) from each other, but did not discriminate patients from healthy controls. When individual genes were regarded, several were found to be differentially expressed between patients and controls. Particularly, strong over-expression of BIK (BCL2-interacting killer) was observed in erythroid progenitor cells of low- and high-risk MDS patients (both p = 0.001) and TNFRSF4 (tumor necrosis factor receptor superfamily 4) was down-regulated in immature hematopoietic cells (p = 0.0023) of low-risk MDS patients compared to healthy bone marrow. Public Library of Science 2016-11-30 /pmc/articles/PMC5130187/ /pubmed/27902785 http://dx.doi.org/10.1371/journal.pone.0165582 Text en © 2016 Langemeijer et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Langemeijer, Saskia MC Mariani, Niccolo Knops, Ruth Gilissen, Christian Woestenenk, Rob de Witte, Theo Huls, Gerwin van der Reijden, Bert A Jansen, Joop H Apoptosis-Related Gene Expression Profiling in Hematopoietic Cell Fractions of MDS Patients |
title | Apoptosis-Related Gene Expression Profiling in Hematopoietic Cell Fractions of MDS Patients |
title_full | Apoptosis-Related Gene Expression Profiling in Hematopoietic Cell Fractions of MDS Patients |
title_fullStr | Apoptosis-Related Gene Expression Profiling in Hematopoietic Cell Fractions of MDS Patients |
title_full_unstemmed | Apoptosis-Related Gene Expression Profiling in Hematopoietic Cell Fractions of MDS Patients |
title_short | Apoptosis-Related Gene Expression Profiling in Hematopoietic Cell Fractions of MDS Patients |
title_sort | apoptosis-related gene expression profiling in hematopoietic cell fractions of mds patients |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5130187/ https://www.ncbi.nlm.nih.gov/pubmed/27902785 http://dx.doi.org/10.1371/journal.pone.0165582 |
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