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Enhancement of Polymerase Activity of the Large Fragment in DNA Polymerase I from Geobacillus stearothermophilus by Site-Directed Mutagenesis at the Active Site

The large fragment of DNA polymerase I from Geobacillus stearothermophilus GIM1.543 (Bst DNA polymerase) with 5′-3′ DNA polymerase activity while in absence of 5′-3′ exonuclease activity possesses high thermal stability and polymerase activity. Bst DNA polymerase was employed in isothermal multiple...

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Detalles Bibliográficos
Autores principales:	Ma, Yi, Zhang, Beilei, Wang, Meng, Ou, Yanghui, Wang, Jufang, Li, Shan
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Hindawi Publishing Corporation 2016
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5131239/ https://www.ncbi.nlm.nih.gov/pubmed/27981047 http://dx.doi.org/10.1155/2016/2906484

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