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Discovery of piperonal-converting oxidase involved in the metabolism of a botanical aromatic aldehyde

Piperonal-catabolizing microorganisms were isolated from soil, the one (strain CT39-3) exhibiting the highest activity being identified as Burkholderia sp. The piperonal-converting enzyme involved in the initial step of piperonal metabolism was purified from strain CT39-3. Gene cloning of the enzyme...

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Autores principales: Doi, Shiori, Hashimoto, Yoshiteru, Tomita, Chiaki, Kumano, Takuto, Kobayashi, Michihiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5131310/
https://www.ncbi.nlm.nih.gov/pubmed/27905507
http://dx.doi.org/10.1038/srep38021
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author Doi, Shiori
Hashimoto, Yoshiteru
Tomita, Chiaki
Kumano, Takuto
Kobayashi, Michihiko
author_facet Doi, Shiori
Hashimoto, Yoshiteru
Tomita, Chiaki
Kumano, Takuto
Kobayashi, Michihiko
author_sort Doi, Shiori
collection PubMed
description Piperonal-catabolizing microorganisms were isolated from soil, the one (strain CT39-3) exhibiting the highest activity being identified as Burkholderia sp. The piperonal-converting enzyme involved in the initial step of piperonal metabolism was purified from strain CT39-3. Gene cloning of the enzyme and a homology search revealed that the enzyme belongs to the xanthine oxidase family, which comprises molybdoenzymes containing a molybdopterin cytosine dinucleotide cofactor. We found that the piperonal-converting enzyme acts on piperonal in the presence of O(2), leading to formation of piperonylic acid and H(2)O(2). The growth of strain CT39-3 was inhibited by higher concentrations of piperonal in the culture medium. Together with this finding, the broad substrate specificity of this enzyme for various aldehydes suggests that it would play an important role in the defense mechanism against antimicrobial compounds derived from plant species.
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spelling pubmed-51313102016-12-15 Discovery of piperonal-converting oxidase involved in the metabolism of a botanical aromatic aldehyde Doi, Shiori Hashimoto, Yoshiteru Tomita, Chiaki Kumano, Takuto Kobayashi, Michihiko Sci Rep Article Piperonal-catabolizing microorganisms were isolated from soil, the one (strain CT39-3) exhibiting the highest activity being identified as Burkholderia sp. The piperonal-converting enzyme involved in the initial step of piperonal metabolism was purified from strain CT39-3. Gene cloning of the enzyme and a homology search revealed that the enzyme belongs to the xanthine oxidase family, which comprises molybdoenzymes containing a molybdopterin cytosine dinucleotide cofactor. We found that the piperonal-converting enzyme acts on piperonal in the presence of O(2), leading to formation of piperonylic acid and H(2)O(2). The growth of strain CT39-3 was inhibited by higher concentrations of piperonal in the culture medium. Together with this finding, the broad substrate specificity of this enzyme for various aldehydes suggests that it would play an important role in the defense mechanism against antimicrobial compounds derived from plant species. Nature Publishing Group 2016-12-01 /pmc/articles/PMC5131310/ /pubmed/27905507 http://dx.doi.org/10.1038/srep38021 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Doi, Shiori
Hashimoto, Yoshiteru
Tomita, Chiaki
Kumano, Takuto
Kobayashi, Michihiko
Discovery of piperonal-converting oxidase involved in the metabolism of a botanical aromatic aldehyde
title Discovery of piperonal-converting oxidase involved in the metabolism of a botanical aromatic aldehyde
title_full Discovery of piperonal-converting oxidase involved in the metabolism of a botanical aromatic aldehyde
title_fullStr Discovery of piperonal-converting oxidase involved in the metabolism of a botanical aromatic aldehyde
title_full_unstemmed Discovery of piperonal-converting oxidase involved in the metabolism of a botanical aromatic aldehyde
title_short Discovery of piperonal-converting oxidase involved in the metabolism of a botanical aromatic aldehyde
title_sort discovery of piperonal-converting oxidase involved in the metabolism of a botanical aromatic aldehyde
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5131310/
https://www.ncbi.nlm.nih.gov/pubmed/27905507
http://dx.doi.org/10.1038/srep38021
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