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Immune-responsiveness of CD4(+) T cells during Streptococcus suis serotype 2 infection

The pathogenesis of Streptococcus suis infection, a major swine and human pathogen, is only partially understood and knowledge on the host adaptive immune response is critically scarce. Yet, S. suis virulence factors, particularly its capsular polysaccharide (CPS), enable this bacterium to modulate...

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Autores principales: Lecours, Marie-Pier, Letendre, Corinne, Clarke, Damian, Lemire, Paul, Galbas, Tristan, Benoit-Biancamano, Marie-Odile, Thibodeau, Jacques, Gottschalk, Marcelo, Segura, Mariela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5131321/
https://www.ncbi.nlm.nih.gov/pubmed/27905502
http://dx.doi.org/10.1038/srep38061
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author Lecours, Marie-Pier
Letendre, Corinne
Clarke, Damian
Lemire, Paul
Galbas, Tristan
Benoit-Biancamano, Marie-Odile
Thibodeau, Jacques
Gottschalk, Marcelo
Segura, Mariela
author_facet Lecours, Marie-Pier
Letendre, Corinne
Clarke, Damian
Lemire, Paul
Galbas, Tristan
Benoit-Biancamano, Marie-Odile
Thibodeau, Jacques
Gottschalk, Marcelo
Segura, Mariela
author_sort Lecours, Marie-Pier
collection PubMed
description The pathogenesis of Streptococcus suis infection, a major swine and human pathogen, is only partially understood and knowledge on the host adaptive immune response is critically scarce. Yet, S. suis virulence factors, particularly its capsular polysaccharide (CPS), enable this bacterium to modulate dendritic cell (DC) functions and potentially impair the immune response. This study aimed to evaluate modulation of T cell activation during S. suis infection and the role of DCs in this response. S. suis-stimulated total mouse splenocytes readily produced TNF-α, IL-6, IFN-γ, CCL3, CXCL9, and IL-10. Ex vivo and in vivo analyses revealed the involvement of CD4(+) T cells and a Th1 response. Nevertheless, during S. suis infection, levels of the Th1-derived cytokines TNF-α and IFN-γ were very low. A transient splenic depletion of CD4(+) T cells and a poor memory response were also observed. Moreover, CD4(+) T cells secreted IL-10 and failed to up-regulate optimal levels of CD40L and CD69 in coculture with DCs. The CPS hampered release of several T cell-derived cytokines in vitro. Finally, a correlation was established between severe clinical signs of S. suis disease and impaired antibody responses. Altogether, these results suggest S. suis interferes with the adaptive immune response.
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spelling pubmed-51313212016-12-15 Immune-responsiveness of CD4(+) T cells during Streptococcus suis serotype 2 infection Lecours, Marie-Pier Letendre, Corinne Clarke, Damian Lemire, Paul Galbas, Tristan Benoit-Biancamano, Marie-Odile Thibodeau, Jacques Gottschalk, Marcelo Segura, Mariela Sci Rep Article The pathogenesis of Streptococcus suis infection, a major swine and human pathogen, is only partially understood and knowledge on the host adaptive immune response is critically scarce. Yet, S. suis virulence factors, particularly its capsular polysaccharide (CPS), enable this bacterium to modulate dendritic cell (DC) functions and potentially impair the immune response. This study aimed to evaluate modulation of T cell activation during S. suis infection and the role of DCs in this response. S. suis-stimulated total mouse splenocytes readily produced TNF-α, IL-6, IFN-γ, CCL3, CXCL9, and IL-10. Ex vivo and in vivo analyses revealed the involvement of CD4(+) T cells and a Th1 response. Nevertheless, during S. suis infection, levels of the Th1-derived cytokines TNF-α and IFN-γ were very low. A transient splenic depletion of CD4(+) T cells and a poor memory response were also observed. Moreover, CD4(+) T cells secreted IL-10 and failed to up-regulate optimal levels of CD40L and CD69 in coculture with DCs. The CPS hampered release of several T cell-derived cytokines in vitro. Finally, a correlation was established between severe clinical signs of S. suis disease and impaired antibody responses. Altogether, these results suggest S. suis interferes with the adaptive immune response. Nature Publishing Group 2016-12-01 /pmc/articles/PMC5131321/ /pubmed/27905502 http://dx.doi.org/10.1038/srep38061 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Lecours, Marie-Pier
Letendre, Corinne
Clarke, Damian
Lemire, Paul
Galbas, Tristan
Benoit-Biancamano, Marie-Odile
Thibodeau, Jacques
Gottschalk, Marcelo
Segura, Mariela
Immune-responsiveness of CD4(+) T cells during Streptococcus suis serotype 2 infection
title Immune-responsiveness of CD4(+) T cells during Streptococcus suis serotype 2 infection
title_full Immune-responsiveness of CD4(+) T cells during Streptococcus suis serotype 2 infection
title_fullStr Immune-responsiveness of CD4(+) T cells during Streptococcus suis serotype 2 infection
title_full_unstemmed Immune-responsiveness of CD4(+) T cells during Streptococcus suis serotype 2 infection
title_short Immune-responsiveness of CD4(+) T cells during Streptococcus suis serotype 2 infection
title_sort immune-responsiveness of cd4(+) t cells during streptococcus suis serotype 2 infection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5131321/
https://www.ncbi.nlm.nih.gov/pubmed/27905502
http://dx.doi.org/10.1038/srep38061
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