Direct identification of antibiotic resistance genes on single plasmid molecules using CRISPR/Cas9 in combination with optical DNA mapping

Bacterial plasmids are extensively involved in the rapid global spread of antibiotic resistance. We here present an assay, based on optical DNA mapping of single plasmids in nanofluidic channels, which provides detailed information about the plasmids present in a bacterial isolate. In a single exper...

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Detalles Bibliográficos
Autores principales: Müller, Vilhelm, Rajer, Fredrika, Frykholm, Karolin, Nyberg, Lena K., Quaderi, Saair, Fritzsche, Joachim, Kristiansson, Erik, Ambjörnsson, Tobias, Sandegren, Linus, Westerlund, Fredrik
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5131345/
https://www.ncbi.nlm.nih.gov/pubmed/27905467
http://dx.doi.org/10.1038/srep37938

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