Cargando…
Laser Speckle Rheology for evaluating the viscoelastic properties of hydrogel scaffolds
Natural and synthetic hydrogel scaffolds exhibit distinct viscoelastic properties at various length scales and deformation rates. Laser Speckle Rheology (LSR) offers a novel, non-contact optical approach for evaluating the frequency-dependent viscoelastic properties of hydrogels. In LSR, a coherent...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5131361/ https://www.ncbi.nlm.nih.gov/pubmed/27905494 http://dx.doi.org/10.1038/srep37949 |
_version_ | 1782470879918161920 |
---|---|
author | Hajjarian, Zeinab Nia, Hadi Tavakoli Ahn, Shawn Grodzinsky, Alan J. Jain, Rakesh K. Nadkarni, Seemantini K. |
author_facet | Hajjarian, Zeinab Nia, Hadi Tavakoli Ahn, Shawn Grodzinsky, Alan J. Jain, Rakesh K. Nadkarni, Seemantini K. |
author_sort | Hajjarian, Zeinab |
collection | PubMed |
description | Natural and synthetic hydrogel scaffolds exhibit distinct viscoelastic properties at various length scales and deformation rates. Laser Speckle Rheology (LSR) offers a novel, non-contact optical approach for evaluating the frequency-dependent viscoelastic properties of hydrogels. In LSR, a coherent laser beam illuminates the specimen and a high-speed camera acquires the time-varying speckle images. Cross-correlation analysis of frames returns the speckle intensity autocorrelation function, g(2)(t), from which the frequency-dependent viscoelastic modulus, G*(ω), is deduced. Here, we establish the capability of LSR for evaluating the viscoelastic properties of hydrogels over a large range of moduli, using conventional mechanical rheometry and atomic force microscopy (AFM)-based indentation as reference-standards. Results demonstrate a strong correlation between |G*(ω)| values measured by LSR and mechanical rheometry (r = 0.95, p < 10(−9)), and z-test analysis reports that moduli values measured by the two methods are identical (p > 0.08) over a large range (47 Pa – 36 kPa). In addition, |G*(ω)| values measured by LSR correlate well with indentation moduli, E, reported by AFM (r = 0.92, p < 10(−7)). Further, spatially-resolved moduli measurements in micro-patterned substrates demonstrate that LSR combines the strengths of conventional rheology and micro-indentation in assessing hydrogel viscoelastic properties at multiple frequencies and small length-scales. |
format | Online Article Text |
id | pubmed-5131361 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-51313612016-12-15 Laser Speckle Rheology for evaluating the viscoelastic properties of hydrogel scaffolds Hajjarian, Zeinab Nia, Hadi Tavakoli Ahn, Shawn Grodzinsky, Alan J. Jain, Rakesh K. Nadkarni, Seemantini K. Sci Rep Article Natural and synthetic hydrogel scaffolds exhibit distinct viscoelastic properties at various length scales and deformation rates. Laser Speckle Rheology (LSR) offers a novel, non-contact optical approach for evaluating the frequency-dependent viscoelastic properties of hydrogels. In LSR, a coherent laser beam illuminates the specimen and a high-speed camera acquires the time-varying speckle images. Cross-correlation analysis of frames returns the speckle intensity autocorrelation function, g(2)(t), from which the frequency-dependent viscoelastic modulus, G*(ω), is deduced. Here, we establish the capability of LSR for evaluating the viscoelastic properties of hydrogels over a large range of moduli, using conventional mechanical rheometry and atomic force microscopy (AFM)-based indentation as reference-standards. Results demonstrate a strong correlation between |G*(ω)| values measured by LSR and mechanical rheometry (r = 0.95, p < 10(−9)), and z-test analysis reports that moduli values measured by the two methods are identical (p > 0.08) over a large range (47 Pa – 36 kPa). In addition, |G*(ω)| values measured by LSR correlate well with indentation moduli, E, reported by AFM (r = 0.92, p < 10(−7)). Further, spatially-resolved moduli measurements in micro-patterned substrates demonstrate that LSR combines the strengths of conventional rheology and micro-indentation in assessing hydrogel viscoelastic properties at multiple frequencies and small length-scales. Nature Publishing Group 2016-12-01 /pmc/articles/PMC5131361/ /pubmed/27905494 http://dx.doi.org/10.1038/srep37949 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Hajjarian, Zeinab Nia, Hadi Tavakoli Ahn, Shawn Grodzinsky, Alan J. Jain, Rakesh K. Nadkarni, Seemantini K. Laser Speckle Rheology for evaluating the viscoelastic properties of hydrogel scaffolds |
title | Laser Speckle Rheology for evaluating the viscoelastic properties of hydrogel scaffolds |
title_full | Laser Speckle Rheology for evaluating the viscoelastic properties of hydrogel scaffolds |
title_fullStr | Laser Speckle Rheology for evaluating the viscoelastic properties of hydrogel scaffolds |
title_full_unstemmed | Laser Speckle Rheology for evaluating the viscoelastic properties of hydrogel scaffolds |
title_short | Laser Speckle Rheology for evaluating the viscoelastic properties of hydrogel scaffolds |
title_sort | laser speckle rheology for evaluating the viscoelastic properties of hydrogel scaffolds |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5131361/ https://www.ncbi.nlm.nih.gov/pubmed/27905494 http://dx.doi.org/10.1038/srep37949 |
work_keys_str_mv | AT hajjarianzeinab laserspecklerheologyforevaluatingtheviscoelasticpropertiesofhydrogelscaffolds AT niahaditavakoli laserspecklerheologyforevaluatingtheviscoelasticpropertiesofhydrogelscaffolds AT ahnshawn laserspecklerheologyforevaluatingtheviscoelasticpropertiesofhydrogelscaffolds AT grodzinskyalanj laserspecklerheologyforevaluatingtheviscoelasticpropertiesofhydrogelscaffolds AT jainrakeshk laserspecklerheologyforevaluatingtheviscoelasticpropertiesofhydrogelscaffolds AT nadkarniseemantinik laserspecklerheologyforevaluatingtheviscoelasticpropertiesofhydrogelscaffolds |