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A New Member of the Growing Family of Contact-Dependent Growth Inhibition Systems in Xenorhabdus doucetiae

Xenorhabdus is a bacterial symbiont of entomopathogenic Steinernema nematodes and is pathogenic for insects. Its life cycle involves a stage inside the insect cadaver, in which it competes for environmental resources with microorganisms from soil and the insect gut. Xenorhabdus is, thus, a useful mo...

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Autores principales: Ogier, Jean-Claude, Duvic, Bernard, Lanois, Anne, Givaudan, Alain, Gaudriault, Sophie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5131962/
https://www.ncbi.nlm.nih.gov/pubmed/27907104
http://dx.doi.org/10.1371/journal.pone.0167443
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author Ogier, Jean-Claude
Duvic, Bernard
Lanois, Anne
Givaudan, Alain
Gaudriault, Sophie
author_facet Ogier, Jean-Claude
Duvic, Bernard
Lanois, Anne
Givaudan, Alain
Gaudriault, Sophie
author_sort Ogier, Jean-Claude
collection PubMed
description Xenorhabdus is a bacterial symbiont of entomopathogenic Steinernema nematodes and is pathogenic for insects. Its life cycle involves a stage inside the insect cadaver, in which it competes for environmental resources with microorganisms from soil and the insect gut. Xenorhabdus is, thus, a useful model for identifying new interbacterial competition systems. For the first time, in an entomopathogenic bacterium, Xenorhabdus doucetiae strain FRM16, we identified a cdi-like locus. The cdi loci encode contact-dependent inhibition (CDI) systems composed of proteins from the two–partner secretion (TPS) family. CdiB is the outer membrane protein and CdiA is the toxic exoprotein. An immunity protein, CdiI, protects bacteria against inhibition. We describe here the growth inhibition effect of the toxic C-terminus of CdiA from X. doucetiae FRM16, CdiA-CT(FRM16), following its production in closely and distantly related enterobacterial species. CdiA-CT(FRM16) displayed Mg(2+)-dependent DNase activity, in vitro. CdiA-CT(FRM16)-mediated growth inhibition was specifically neutralized by CdiI(FRM16). Moreover, the cdi (FRM16) locus encodes an ortholog of toxin-activating proteins C that we named CdiC(FRM16). In addition to E. coli, the cdiBCAI-type locus was found to be widespread in environmental bacteria interacting with insects, plants, rhizospheres and soils. Phylogenetic tree comparisons for CdiB, CdiA and CdiC suggested that the genes encoding these proteins had co-evolved. By contrast, the considerable variability of CdiI protein sequences suggests that the cdiI gene is an independent evolutionary unit. These findings further characterize the sparsely described cdiBCAI-type locus.
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spelling pubmed-51319622016-12-21 A New Member of the Growing Family of Contact-Dependent Growth Inhibition Systems in Xenorhabdus doucetiae Ogier, Jean-Claude Duvic, Bernard Lanois, Anne Givaudan, Alain Gaudriault, Sophie PLoS One Research Article Xenorhabdus is a bacterial symbiont of entomopathogenic Steinernema nematodes and is pathogenic for insects. Its life cycle involves a stage inside the insect cadaver, in which it competes for environmental resources with microorganisms from soil and the insect gut. Xenorhabdus is, thus, a useful model for identifying new interbacterial competition systems. For the first time, in an entomopathogenic bacterium, Xenorhabdus doucetiae strain FRM16, we identified a cdi-like locus. The cdi loci encode contact-dependent inhibition (CDI) systems composed of proteins from the two–partner secretion (TPS) family. CdiB is the outer membrane protein and CdiA is the toxic exoprotein. An immunity protein, CdiI, protects bacteria against inhibition. We describe here the growth inhibition effect of the toxic C-terminus of CdiA from X. doucetiae FRM16, CdiA-CT(FRM16), following its production in closely and distantly related enterobacterial species. CdiA-CT(FRM16) displayed Mg(2+)-dependent DNase activity, in vitro. CdiA-CT(FRM16)-mediated growth inhibition was specifically neutralized by CdiI(FRM16). Moreover, the cdi (FRM16) locus encodes an ortholog of toxin-activating proteins C that we named CdiC(FRM16). In addition to E. coli, the cdiBCAI-type locus was found to be widespread in environmental bacteria interacting with insects, plants, rhizospheres and soils. Phylogenetic tree comparisons for CdiB, CdiA and CdiC suggested that the genes encoding these proteins had co-evolved. By contrast, the considerable variability of CdiI protein sequences suggests that the cdiI gene is an independent evolutionary unit. These findings further characterize the sparsely described cdiBCAI-type locus. Public Library of Science 2016-12-01 /pmc/articles/PMC5131962/ /pubmed/27907104 http://dx.doi.org/10.1371/journal.pone.0167443 Text en © 2016 Ogier et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Ogier, Jean-Claude
Duvic, Bernard
Lanois, Anne
Givaudan, Alain
Gaudriault, Sophie
A New Member of the Growing Family of Contact-Dependent Growth Inhibition Systems in Xenorhabdus doucetiae
title A New Member of the Growing Family of Contact-Dependent Growth Inhibition Systems in Xenorhabdus doucetiae
title_full A New Member of the Growing Family of Contact-Dependent Growth Inhibition Systems in Xenorhabdus doucetiae
title_fullStr A New Member of the Growing Family of Contact-Dependent Growth Inhibition Systems in Xenorhabdus doucetiae
title_full_unstemmed A New Member of the Growing Family of Contact-Dependent Growth Inhibition Systems in Xenorhabdus doucetiae
title_short A New Member of the Growing Family of Contact-Dependent Growth Inhibition Systems in Xenorhabdus doucetiae
title_sort new member of the growing family of contact-dependent growth inhibition systems in xenorhabdus doucetiae
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5131962/
https://www.ncbi.nlm.nih.gov/pubmed/27907104
http://dx.doi.org/10.1371/journal.pone.0167443
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