Cargando…

Parenchymal and Stromal Cells Contribute to Pro-Inflammatory Myocardial Environment at Early Stages of Diabetes: Protective Role of Resveratrol

Background: Little information is currently available concerning the relative contribution of cardiac parenchymal and stromal cells in the activation of the pro-inflammatory signal cascade, at the initial stages of diabetes. Similarly, the effects of early resveratrol (RSV) treatment on the negative...

Descripción completa

Detalles Bibliográficos
Autores principales: Savi, Monia, Bocchi, Leonardo, Sala, Roberto, Frati, Caterina, Lagrasta, Costanza, Madeddu, Denise, Falco, Angela, Pollino, Serena, Bresciani, Letizia, Miragoli, Michele, Zaniboni, Massimiliano, Quaini, Federico, Del Rio, Daniele, Stilli, Donatella
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5133113/
https://www.ncbi.nlm.nih.gov/pubmed/27854328
http://dx.doi.org/10.3390/nu8110729
_version_ 1782471212515983360
author Savi, Monia
Bocchi, Leonardo
Sala, Roberto
Frati, Caterina
Lagrasta, Costanza
Madeddu, Denise
Falco, Angela
Pollino, Serena
Bresciani, Letizia
Miragoli, Michele
Zaniboni, Massimiliano
Quaini, Federico
Del Rio, Daniele
Stilli, Donatella
author_facet Savi, Monia
Bocchi, Leonardo
Sala, Roberto
Frati, Caterina
Lagrasta, Costanza
Madeddu, Denise
Falco, Angela
Pollino, Serena
Bresciani, Letizia
Miragoli, Michele
Zaniboni, Massimiliano
Quaini, Federico
Del Rio, Daniele
Stilli, Donatella
author_sort Savi, Monia
collection PubMed
description Background: Little information is currently available concerning the relative contribution of cardiac parenchymal and stromal cells in the activation of the pro-inflammatory signal cascade, at the initial stages of diabetes. Similarly, the effects of early resveratrol (RSV) treatment on the negative impact of diabetes on the different myocardial cell compartments remain to be defined. Methods: In vitro challenge of neonatal cardiomyocytes and fibroblasts to high glucose and in vivo/ex vivo experiments on a rat model of Streptozotocin-induced diabetes were used to specifically address these issues. Results: In vitro data indicated that, besides cardiomyocytes, neonatal fibroblasts contribute to generating initial changes in the myocardial environment, in terms of pro-inflammatory cytokine expression. These findings were mostly confirmed at the myocardial tissue level in diabetic rats, after three weeks of hyperglycemia. Specifically, monocyte chemoattractant protein-1 and Fractalkine were up-regulated and initial abnormalities in cardiomyocyte contractility occurred. At later stages of diabetes, a selective enhancement of pro-inflammatory macrophage M1 phenotype and a parallel reduction of anti-inflammatory macrophage M2 phenotype were associated with a marked disorganization of cardiomyocyte ultrastructural properties. RSV treatment inhibited pro-inflammatory cytokine production, leading to a recovery of cardiomyocyte contractile efficiency and a reduced inflammatory cell recruitment. Conclusion: Early RSV administration could inhibit the pro-inflammatory diabetic milieu sustained by different cardiac cell types.
format Online
Article
Text
id pubmed-5133113
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-51331132016-12-11 Parenchymal and Stromal Cells Contribute to Pro-Inflammatory Myocardial Environment at Early Stages of Diabetes: Protective Role of Resveratrol Savi, Monia Bocchi, Leonardo Sala, Roberto Frati, Caterina Lagrasta, Costanza Madeddu, Denise Falco, Angela Pollino, Serena Bresciani, Letizia Miragoli, Michele Zaniboni, Massimiliano Quaini, Federico Del Rio, Daniele Stilli, Donatella Nutrients Article Background: Little information is currently available concerning the relative contribution of cardiac parenchymal and stromal cells in the activation of the pro-inflammatory signal cascade, at the initial stages of diabetes. Similarly, the effects of early resveratrol (RSV) treatment on the negative impact of diabetes on the different myocardial cell compartments remain to be defined. Methods: In vitro challenge of neonatal cardiomyocytes and fibroblasts to high glucose and in vivo/ex vivo experiments on a rat model of Streptozotocin-induced diabetes were used to specifically address these issues. Results: In vitro data indicated that, besides cardiomyocytes, neonatal fibroblasts contribute to generating initial changes in the myocardial environment, in terms of pro-inflammatory cytokine expression. These findings were mostly confirmed at the myocardial tissue level in diabetic rats, after three weeks of hyperglycemia. Specifically, monocyte chemoattractant protein-1 and Fractalkine were up-regulated and initial abnormalities in cardiomyocyte contractility occurred. At later stages of diabetes, a selective enhancement of pro-inflammatory macrophage M1 phenotype and a parallel reduction of anti-inflammatory macrophage M2 phenotype were associated with a marked disorganization of cardiomyocyte ultrastructural properties. RSV treatment inhibited pro-inflammatory cytokine production, leading to a recovery of cardiomyocyte contractile efficiency and a reduced inflammatory cell recruitment. Conclusion: Early RSV administration could inhibit the pro-inflammatory diabetic milieu sustained by different cardiac cell types. MDPI 2016-11-16 /pmc/articles/PMC5133113/ /pubmed/27854328 http://dx.doi.org/10.3390/nu8110729 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Savi, Monia
Bocchi, Leonardo
Sala, Roberto
Frati, Caterina
Lagrasta, Costanza
Madeddu, Denise
Falco, Angela
Pollino, Serena
Bresciani, Letizia
Miragoli, Michele
Zaniboni, Massimiliano
Quaini, Federico
Del Rio, Daniele
Stilli, Donatella
Parenchymal and Stromal Cells Contribute to Pro-Inflammatory Myocardial Environment at Early Stages of Diabetes: Protective Role of Resveratrol
title Parenchymal and Stromal Cells Contribute to Pro-Inflammatory Myocardial Environment at Early Stages of Diabetes: Protective Role of Resveratrol
title_full Parenchymal and Stromal Cells Contribute to Pro-Inflammatory Myocardial Environment at Early Stages of Diabetes: Protective Role of Resveratrol
title_fullStr Parenchymal and Stromal Cells Contribute to Pro-Inflammatory Myocardial Environment at Early Stages of Diabetes: Protective Role of Resveratrol
title_full_unstemmed Parenchymal and Stromal Cells Contribute to Pro-Inflammatory Myocardial Environment at Early Stages of Diabetes: Protective Role of Resveratrol
title_short Parenchymal and Stromal Cells Contribute to Pro-Inflammatory Myocardial Environment at Early Stages of Diabetes: Protective Role of Resveratrol
title_sort parenchymal and stromal cells contribute to pro-inflammatory myocardial environment at early stages of diabetes: protective role of resveratrol
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5133113/
https://www.ncbi.nlm.nih.gov/pubmed/27854328
http://dx.doi.org/10.3390/nu8110729
work_keys_str_mv AT savimonia parenchymalandstromalcellscontributetoproinflammatorymyocardialenvironmentatearlystagesofdiabetesprotectiveroleofresveratrol
AT bocchileonardo parenchymalandstromalcellscontributetoproinflammatorymyocardialenvironmentatearlystagesofdiabetesprotectiveroleofresveratrol
AT salaroberto parenchymalandstromalcellscontributetoproinflammatorymyocardialenvironmentatearlystagesofdiabetesprotectiveroleofresveratrol
AT fraticaterina parenchymalandstromalcellscontributetoproinflammatorymyocardialenvironmentatearlystagesofdiabetesprotectiveroleofresveratrol
AT lagrastacostanza parenchymalandstromalcellscontributetoproinflammatorymyocardialenvironmentatearlystagesofdiabetesprotectiveroleofresveratrol
AT madeddudenise parenchymalandstromalcellscontributetoproinflammatorymyocardialenvironmentatearlystagesofdiabetesprotectiveroleofresveratrol
AT falcoangela parenchymalandstromalcellscontributetoproinflammatorymyocardialenvironmentatearlystagesofdiabetesprotectiveroleofresveratrol
AT pollinoserena parenchymalandstromalcellscontributetoproinflammatorymyocardialenvironmentatearlystagesofdiabetesprotectiveroleofresveratrol
AT brescianiletizia parenchymalandstromalcellscontributetoproinflammatorymyocardialenvironmentatearlystagesofdiabetesprotectiveroleofresveratrol
AT miragolimichele parenchymalandstromalcellscontributetoproinflammatorymyocardialenvironmentatearlystagesofdiabetesprotectiveroleofresveratrol
AT zanibonimassimiliano parenchymalandstromalcellscontributetoproinflammatorymyocardialenvironmentatearlystagesofdiabetesprotectiveroleofresveratrol
AT quainifederico parenchymalandstromalcellscontributetoproinflammatorymyocardialenvironmentatearlystagesofdiabetesprotectiveroleofresveratrol
AT delriodaniele parenchymalandstromalcellscontributetoproinflammatorymyocardialenvironmentatearlystagesofdiabetesprotectiveroleofresveratrol
AT stillidonatella parenchymalandstromalcellscontributetoproinflammatorymyocardialenvironmentatearlystagesofdiabetesprotectiveroleofresveratrol