Cargando…

The miR-125 family is an important regulator of the expression and maintenance of maternal effect genes during preimplantational embryo development

Previously, we reported that Sebox is a new maternal effect gene (MEG) that is required for early embryo development beyond the two-cell (2C) stage because this gene orchestrates the expression of important genes for zygotic genome activation (ZGA). However, regulators of Sebox expression remain unk...

Descripción completa

Detalles Bibliográficos
Autores principales: Kim, Kyeoung-Hwa, Seo, You-Mi, Kim, Eun-Young, Lee, Su-Yeon, Kwon, Jini, Ko, Jung-Jae, Lee, Kyung-Ah
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5133438/
https://www.ncbi.nlm.nih.gov/pubmed/27906131
http://dx.doi.org/10.1098/rsob.160181
_version_ 1782471262417715200
author Kim, Kyeoung-Hwa
Seo, You-Mi
Kim, Eun-Young
Lee, Su-Yeon
Kwon, Jini
Ko, Jung-Jae
Lee, Kyung-Ah
author_facet Kim, Kyeoung-Hwa
Seo, You-Mi
Kim, Eun-Young
Lee, Su-Yeon
Kwon, Jini
Ko, Jung-Jae
Lee, Kyung-Ah
author_sort Kim, Kyeoung-Hwa
collection PubMed
description Previously, we reported that Sebox is a new maternal effect gene (MEG) that is required for early embryo development beyond the two-cell (2C) stage because this gene orchestrates the expression of important genes for zygotic genome activation (ZGA). However, regulators of Sebox expression remain unknown. Therefore, the objectives of the present study were to use bioinformatics tools to identify such regulatory microRNAs (miRNAs) and to determine the effects of the identified miRNAs on Sebox expression. Using computational algorithms, we identified a motif within the 3′UTR of Sebox mRNA that is specific to the seed region of the miR-125 family, which includes miR-125a-5p, miR-125b-5p and miR-351-5p. During our search for miRNAs, we found that the Lin28a 3′UTR also contains the same binding motif for the seed region of the miR-125 family. In addition, we confirmed that Lin28a also plays a role as a MEG and affects ZGA at the 2C stage, without affecting oocyte maturation or fertilization. Thus, we provide the first report indicating that the miR-125 family plays a crucial role in regulating MEGs related to the 2C block and in regulating ZGA through methods such as affecting Sebox and Lin28a in oocytes and embryos.
format Online
Article
Text
id pubmed-5133438
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher The Royal Society
record_format MEDLINE/PubMed
spelling pubmed-51334382016-12-12 The miR-125 family is an important regulator of the expression and maintenance of maternal effect genes during preimplantational embryo development Kim, Kyeoung-Hwa Seo, You-Mi Kim, Eun-Young Lee, Su-Yeon Kwon, Jini Ko, Jung-Jae Lee, Kyung-Ah Open Biol Research Previously, we reported that Sebox is a new maternal effect gene (MEG) that is required for early embryo development beyond the two-cell (2C) stage because this gene orchestrates the expression of important genes for zygotic genome activation (ZGA). However, regulators of Sebox expression remain unknown. Therefore, the objectives of the present study were to use bioinformatics tools to identify such regulatory microRNAs (miRNAs) and to determine the effects of the identified miRNAs on Sebox expression. Using computational algorithms, we identified a motif within the 3′UTR of Sebox mRNA that is specific to the seed region of the miR-125 family, which includes miR-125a-5p, miR-125b-5p and miR-351-5p. During our search for miRNAs, we found that the Lin28a 3′UTR also contains the same binding motif for the seed region of the miR-125 family. In addition, we confirmed that Lin28a also plays a role as a MEG and affects ZGA at the 2C stage, without affecting oocyte maturation or fertilization. Thus, we provide the first report indicating that the miR-125 family plays a crucial role in regulating MEGs related to the 2C block and in regulating ZGA through methods such as affecting Sebox and Lin28a in oocytes and embryos. The Royal Society 2016-11-30 /pmc/articles/PMC5133438/ /pubmed/27906131 http://dx.doi.org/10.1098/rsob.160181 Text en © 2016 The Authors. http://creativecommons.org/licenses/by/4.0/ Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.
spellingShingle Research
Kim, Kyeoung-Hwa
Seo, You-Mi
Kim, Eun-Young
Lee, Su-Yeon
Kwon, Jini
Ko, Jung-Jae
Lee, Kyung-Ah
The miR-125 family is an important regulator of the expression and maintenance of maternal effect genes during preimplantational embryo development
title The miR-125 family is an important regulator of the expression and maintenance of maternal effect genes during preimplantational embryo development
title_full The miR-125 family is an important regulator of the expression and maintenance of maternal effect genes during preimplantational embryo development
title_fullStr The miR-125 family is an important regulator of the expression and maintenance of maternal effect genes during preimplantational embryo development
title_full_unstemmed The miR-125 family is an important regulator of the expression and maintenance of maternal effect genes during preimplantational embryo development
title_short The miR-125 family is an important regulator of the expression and maintenance of maternal effect genes during preimplantational embryo development
title_sort mir-125 family is an important regulator of the expression and maintenance of maternal effect genes during preimplantational embryo development
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5133438/
https://www.ncbi.nlm.nih.gov/pubmed/27906131
http://dx.doi.org/10.1098/rsob.160181
work_keys_str_mv AT kimkyeounghwa themir125familyisanimportantregulatoroftheexpressionandmaintenanceofmaternaleffectgenesduringpreimplantationalembryodevelopment
AT seoyoumi themir125familyisanimportantregulatoroftheexpressionandmaintenanceofmaternaleffectgenesduringpreimplantationalembryodevelopment
AT kimeunyoung themir125familyisanimportantregulatoroftheexpressionandmaintenanceofmaternaleffectgenesduringpreimplantationalembryodevelopment
AT leesuyeon themir125familyisanimportantregulatoroftheexpressionandmaintenanceofmaternaleffectgenesduringpreimplantationalembryodevelopment
AT kwonjini themir125familyisanimportantregulatoroftheexpressionandmaintenanceofmaternaleffectgenesduringpreimplantationalembryodevelopment
AT kojungjae themir125familyisanimportantregulatoroftheexpressionandmaintenanceofmaternaleffectgenesduringpreimplantationalembryodevelopment
AT leekyungah themir125familyisanimportantregulatoroftheexpressionandmaintenanceofmaternaleffectgenesduringpreimplantationalembryodevelopment
AT kimkyeounghwa mir125familyisanimportantregulatoroftheexpressionandmaintenanceofmaternaleffectgenesduringpreimplantationalembryodevelopment
AT seoyoumi mir125familyisanimportantregulatoroftheexpressionandmaintenanceofmaternaleffectgenesduringpreimplantationalembryodevelopment
AT kimeunyoung mir125familyisanimportantregulatoroftheexpressionandmaintenanceofmaternaleffectgenesduringpreimplantationalembryodevelopment
AT leesuyeon mir125familyisanimportantregulatoroftheexpressionandmaintenanceofmaternaleffectgenesduringpreimplantationalembryodevelopment
AT kwonjini mir125familyisanimportantregulatoroftheexpressionandmaintenanceofmaternaleffectgenesduringpreimplantationalembryodevelopment
AT kojungjae mir125familyisanimportantregulatoroftheexpressionandmaintenanceofmaternaleffectgenesduringpreimplantationalembryodevelopment
AT leekyungah mir125familyisanimportantregulatoroftheexpressionandmaintenanceofmaternaleffectgenesduringpreimplantationalembryodevelopment