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Genomic variations leading to alterations in cell morphology of Campylobacter spp

Campylobacter jejuni, the most common cause of bacterial diarrhoeal disease, is normally helical. However, it can also adopt straight rod, elongated helical and coccoid forms. Studying how helical morphology is generated, and how it switches between its different forms, is an important objective for...

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Autores principales: Esson, Diane, Mather, Alison E., Scanlan, Eoin, Gupta, Srishti, de Vries, Stefan P. W., Bailey, David, Harris, Simon R., McKinley, Trevelyan J., Méric, Guillaume, Berry, Sophia K., Mastroeni, Pietro, Sheppard, Samuel K., Christie, Graham, Thomson, Nicholas R., Parkhill, Julian, Maskell, Duncan J., Grant, Andrew J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5133587/
https://www.ncbi.nlm.nih.gov/pubmed/27910897
http://dx.doi.org/10.1038/srep38303
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author Esson, Diane
Mather, Alison E.
Scanlan, Eoin
Gupta, Srishti
de Vries, Stefan P. W.
Bailey, David
Harris, Simon R.
McKinley, Trevelyan J.
Méric, Guillaume
Berry, Sophia K.
Mastroeni, Pietro
Sheppard, Samuel K.
Christie, Graham
Thomson, Nicholas R.
Parkhill, Julian
Maskell, Duncan J.
Grant, Andrew J.
author_facet Esson, Diane
Mather, Alison E.
Scanlan, Eoin
Gupta, Srishti
de Vries, Stefan P. W.
Bailey, David
Harris, Simon R.
McKinley, Trevelyan J.
Méric, Guillaume
Berry, Sophia K.
Mastroeni, Pietro
Sheppard, Samuel K.
Christie, Graham
Thomson, Nicholas R.
Parkhill, Julian
Maskell, Duncan J.
Grant, Andrew J.
author_sort Esson, Diane
collection PubMed
description Campylobacter jejuni, the most common cause of bacterial diarrhoeal disease, is normally helical. However, it can also adopt straight rod, elongated helical and coccoid forms. Studying how helical morphology is generated, and how it switches between its different forms, is an important objective for understanding this pathogen. Here, we aimed to determine the genetic factors involved in generating the helical shape of Campylobacter. A C. jejuni transposon (Tn) mutant library was screened for non-helical mutants with inconsistent results. Whole genome sequence variation and morphological trends within this Tn library, and in various C. jejuni wild type strains, were compared and correlated to detect genomic elements associated with helical and rod morphologies. All rod-shaped C. jejuni Tn mutants and all rod-shaped laboratory, clinical and environmental C. jejuni and Campylobacter coli contained genetic changes within the pgp1 or pgp2 genes, which encode peptidoglycan modifying enzymes. We therefore confirm the importance of Pgp1 and Pgp2 in the maintenance of helical shape and extended this to a wide range of C. jejuni and C. coli isolates. Genome sequence analysis revealed variation in the sequence and length of homopolymeric tracts found within these genes, providing a potential mechanism of phase variation of cell shape.
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spelling pubmed-51335872017-01-27 Genomic variations leading to alterations in cell morphology of Campylobacter spp Esson, Diane Mather, Alison E. Scanlan, Eoin Gupta, Srishti de Vries, Stefan P. W. Bailey, David Harris, Simon R. McKinley, Trevelyan J. Méric, Guillaume Berry, Sophia K. Mastroeni, Pietro Sheppard, Samuel K. Christie, Graham Thomson, Nicholas R. Parkhill, Julian Maskell, Duncan J. Grant, Andrew J. Sci Rep Article Campylobacter jejuni, the most common cause of bacterial diarrhoeal disease, is normally helical. However, it can also adopt straight rod, elongated helical and coccoid forms. Studying how helical morphology is generated, and how it switches between its different forms, is an important objective for understanding this pathogen. Here, we aimed to determine the genetic factors involved in generating the helical shape of Campylobacter. A C. jejuni transposon (Tn) mutant library was screened for non-helical mutants with inconsistent results. Whole genome sequence variation and morphological trends within this Tn library, and in various C. jejuni wild type strains, were compared and correlated to detect genomic elements associated with helical and rod morphologies. All rod-shaped C. jejuni Tn mutants and all rod-shaped laboratory, clinical and environmental C. jejuni and Campylobacter coli contained genetic changes within the pgp1 or pgp2 genes, which encode peptidoglycan modifying enzymes. We therefore confirm the importance of Pgp1 and Pgp2 in the maintenance of helical shape and extended this to a wide range of C. jejuni and C. coli isolates. Genome sequence analysis revealed variation in the sequence and length of homopolymeric tracts found within these genes, providing a potential mechanism of phase variation of cell shape. Nature Publishing Group 2016-12-02 /pmc/articles/PMC5133587/ /pubmed/27910897 http://dx.doi.org/10.1038/srep38303 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Esson, Diane
Mather, Alison E.
Scanlan, Eoin
Gupta, Srishti
de Vries, Stefan P. W.
Bailey, David
Harris, Simon R.
McKinley, Trevelyan J.
Méric, Guillaume
Berry, Sophia K.
Mastroeni, Pietro
Sheppard, Samuel K.
Christie, Graham
Thomson, Nicholas R.
Parkhill, Julian
Maskell, Duncan J.
Grant, Andrew J.
Genomic variations leading to alterations in cell morphology of Campylobacter spp
title Genomic variations leading to alterations in cell morphology of Campylobacter spp
title_full Genomic variations leading to alterations in cell morphology of Campylobacter spp
title_fullStr Genomic variations leading to alterations in cell morphology of Campylobacter spp
title_full_unstemmed Genomic variations leading to alterations in cell morphology of Campylobacter spp
title_short Genomic variations leading to alterations in cell morphology of Campylobacter spp
title_sort genomic variations leading to alterations in cell morphology of campylobacter spp
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5133587/
https://www.ncbi.nlm.nih.gov/pubmed/27910897
http://dx.doi.org/10.1038/srep38303
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