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Mass spectrometry profiling of oxysterols in human sperm identifies 25-hydroxycholesterol as a marker of sperm function
Cholesterol is a main lipid component of sperm cell that is essential for sperm membrane fluidity, capacitation, and acrosomal reaction. Recent data obtained in bovine sperm showed that sperm capacitation is associated to the formation of oxysterols, oxidized products of cholesterol. The aim of this...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5133663/ https://www.ncbi.nlm.nih.gov/pubmed/27912195 http://dx.doi.org/10.1016/j.redox.2016.11.008 |
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author | Zerbinati, Chiara Caponecchia, Luisa Puca, Rosa Ciacciarelli, Marco Salacone, Pietro Sebastianelli, Annalisa Pastore, Antonio Palleschi, Giovanni Petrozza, Vincenzo Porta, Natale Rago, Rocco Carbone, Antonio Iuliano, Luigi |
author_facet | Zerbinati, Chiara Caponecchia, Luisa Puca, Rosa Ciacciarelli, Marco Salacone, Pietro Sebastianelli, Annalisa Pastore, Antonio Palleschi, Giovanni Petrozza, Vincenzo Porta, Natale Rago, Rocco Carbone, Antonio Iuliano, Luigi |
author_sort | Zerbinati, Chiara |
collection | PubMed |
description | Cholesterol is a main lipid component of sperm cell that is essential for sperm membrane fluidity, capacitation, and acrosomal reaction. Recent data obtained in bovine sperm showed that sperm capacitation is associated to the formation of oxysterols, oxidized products of cholesterol. The aim of this study was to profile oxysterol content in human semen, and to investigate their potential role in sperm pathophysiology. Among the 12 oxysterols analyzed, 25-hydroxycholesterol (25-HC) resulted the most represented in normozoospermic samples, and its concentration positively correlated with spermatozoa number. We detected Cholesterol 25-hydroxylase, the enzyme responsible for 25-HC production, in human spermatozoa at the level of the neck and the post acrosomal area. Upon incubation with spermatozoa, 25-HC induced calcium and cholesterol transients in connection with the acrosomal reaction. Our results support a role for 25-HC in sperm function. |
format | Online Article Text |
id | pubmed-5133663 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-51336632016-12-09 Mass spectrometry profiling of oxysterols in human sperm identifies 25-hydroxycholesterol as a marker of sperm function Zerbinati, Chiara Caponecchia, Luisa Puca, Rosa Ciacciarelli, Marco Salacone, Pietro Sebastianelli, Annalisa Pastore, Antonio Palleschi, Giovanni Petrozza, Vincenzo Porta, Natale Rago, Rocco Carbone, Antonio Iuliano, Luigi Redox Biol Method Cholesterol is a main lipid component of sperm cell that is essential for sperm membrane fluidity, capacitation, and acrosomal reaction. Recent data obtained in bovine sperm showed that sperm capacitation is associated to the formation of oxysterols, oxidized products of cholesterol. The aim of this study was to profile oxysterol content in human semen, and to investigate their potential role in sperm pathophysiology. Among the 12 oxysterols analyzed, 25-hydroxycholesterol (25-HC) resulted the most represented in normozoospermic samples, and its concentration positively correlated with spermatozoa number. We detected Cholesterol 25-hydroxylase, the enzyme responsible for 25-HC production, in human spermatozoa at the level of the neck and the post acrosomal area. Upon incubation with spermatozoa, 25-HC induced calcium and cholesterol transients in connection with the acrosomal reaction. Our results support a role for 25-HC in sperm function. Elsevier 2016-11-22 /pmc/articles/PMC5133663/ /pubmed/27912195 http://dx.doi.org/10.1016/j.redox.2016.11.008 Text en © 2016 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Method Zerbinati, Chiara Caponecchia, Luisa Puca, Rosa Ciacciarelli, Marco Salacone, Pietro Sebastianelli, Annalisa Pastore, Antonio Palleschi, Giovanni Petrozza, Vincenzo Porta, Natale Rago, Rocco Carbone, Antonio Iuliano, Luigi Mass spectrometry profiling of oxysterols in human sperm identifies 25-hydroxycholesterol as a marker of sperm function |
title | Mass spectrometry profiling of oxysterols in human sperm identifies 25-hydroxycholesterol as a marker of sperm function |
title_full | Mass spectrometry profiling of oxysterols in human sperm identifies 25-hydroxycholesterol as a marker of sperm function |
title_fullStr | Mass spectrometry profiling of oxysterols in human sperm identifies 25-hydroxycholesterol as a marker of sperm function |
title_full_unstemmed | Mass spectrometry profiling of oxysterols in human sperm identifies 25-hydroxycholesterol as a marker of sperm function |
title_short | Mass spectrometry profiling of oxysterols in human sperm identifies 25-hydroxycholesterol as a marker of sperm function |
title_sort | mass spectrometry profiling of oxysterols in human sperm identifies 25-hydroxycholesterol as a marker of sperm function |
topic | Method |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5133663/ https://www.ncbi.nlm.nih.gov/pubmed/27912195 http://dx.doi.org/10.1016/j.redox.2016.11.008 |
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