Cargando…

A Melting Curve-Based Multiplex RT-qPCR Assay for Simultaneous Detection of Four Human Coronaviruses

Human coronaviruses HCoV-OC43, HCoV-229E, HCoV-NL63 and HCoV-HKU1 are common respiratory viruses associated with acute respiratory infection. They have a global distribution. Rapid and accurate diagnosis of HCoV infection is important for the management and treatment of hospitalized patients with HC...

Descripción completa

Detalles Bibliográficos
Autores principales:	Wan, Zhenzhou, Zhang, Ya’nan, He, Zhixiang, Liu, Jia, Lan, Ke, Hu, Yihong, Zhang, Chiyu
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2016
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5133880/ https://www.ncbi.nlm.nih.gov/pubmed/27886052 http://dx.doi.org/10.3390/ijms17111880

Ejemplares similares

A new multiplex RT-qPCR method for the simultaneous detection and discrimination of Zika and chikungunya viruses
por: Broeders, Sylvia, et al.
Publicado: (2020)

Validation and advantages of using novel RT-qPCR melting curve analysis assays for the identification of SARS-CoV-2 variants
por: Juul, Sebastian, et al.
Publicado: (2022)

Development of a Sensitive and Specific Novel qPCR Assay for Simultaneous Detection and Differentiation of Mucormycosis and Aspergillosis by Melting Curve Analysis
por: Pandey, Mragnayani, et al.
Publicado: (2022)

Detection of SARS-CoV-2 RNA by multiplex RT-qPCR
por: Kudo, Eriko, et al.
Publicado: (2020)

In-silico prediction of RT-qPCR-high resolution melting for broad detection of emaraviruses
por: Olmedo-Velarde, Alejandro, et al.
Publicado: (2023)

A sensitive and affordable multiplex RT-qPCR assay for SARS-CoV-2 detection
por: Reijns, Martin A. M., et al.
Publicado: (2020)

Development of quantitative multiplex RT-qPCR one step assay for detection of hepatitis delta virus
por: da Silva Queiroz, Jackson Alves, et al.
Publicado: (2023)

Specific and Sensitive Detection of Neuroblastoma mRNA Markers by Multiplex RT-qPCR
por: van Zogchel, Lieke M. J., et al.
Publicado: (2021)

Hundreds-Dollar-Level Multiplex Integrated RT-qPCR Quantitative System for Field Detection
por: Lan, Zhihao, et al.
Publicado: (2022)

Web-based LinRegPCR: application for the visualization and analysis of (RT)-qPCR amplification and melting data
por: Untergasser, Andreas, et al.
Publicado: (2021)

Multiplex One-Step RT-qPCR Assays for Simultaneous Detection of SARS-CoV-2 and Other Enteric Viruses of Dogs and Cats
por: Thieulent, Côme J., et al.
Publicado: (2023)

A novel multiplex qPCR‑HRM assay for the simultaneous detection of four abortive zoonotic agents in cattle, sheep, and goats
por: Modise, Boitumelo M., et al.
Publicado: (2023)

A TaqMan-probe-based multiplex real-time RT-qPCR for simultaneous detection of porcine enteric coronaviruses
por: Huang, Xin, et al.
Publicado: (2019)

MeltMan: Optimization, Evaluation, and Universal Application of a qPCR System Integrating the TaqMan qPCR and Melting Analysis into a Single Assay
por: Nagy, Alexander, et al.
Publicado: (2016)

Multiplex Detection of Salmonella spp., E. coli O157 and L. monocytogenes by qPCR Melt Curve Analysis in Spiked Infant Formula
por: Azinheiro, Sarah, et al.
Publicado: (2020)

Development of a Multiplex RT-qPCR for the Detection of Different Clades of Avian Influenza in Poultry
por: Le, Tran Bac, et al.
Publicado: (2020)

Multiplex RT-qPCR Application in Early Detection of Bovine Respiratory Disease in Healthy Calves
por: Goto, Yusuke, et al.
Publicado: (2023)

Sample-ready multiplex qPCR assay for detection of malaria
por: Kamau, Edwin, et al.
Publicado: (2014)

Evaluation of the mechanism of action of Bacillus spp. to manage Meloidogyne incognita with split root assay, RT-qPCR and qPCR
por: Gattoni, Kaitlin M., et al.
Publicado: (2023)

Correction: MeltMan: Optimization, Evaluation, and Universal Application of a qPCR System Integrating the TaqMan qPCR and Melting Analysis into a Single Assay
por: Nagy, Alexander, et al.
Publicado: (2018)

Applicability of RNA standards for evaluating RT-qPCR assays and platforms
por: Devonshire, Alison S, et al.
Publicado: (2011)

Hepatitis A virus subgenotyping based on RT-qPCR assays
por: Coudray-Meunier, Coralie, et al.
Publicado: (2014)

Tutorial: Guidelines for Single-Cell RT-qPCR
por: Zucha, Daniel, et al.
Publicado: (2021)

Development of RT-qPCR and semi-nested RT-PCR assays for molecular diagnosis of hantavirus pulmonary syndrome
por: Nunes, Bruno Tardelli Diniz, et al.
Publicado: (2019)

A multiplex RT-PCR assay for rapid and simultaneous detection of four RNA viruses in swine
por: Zhao, Yan, et al.
Publicado: (2019)

Development and Validation of a Panel of One-Step Four-Plex qPCR/RT-qPCR Assays for Simultaneous Detection of SARS-CoV-2 and Other Pathogens Associated with Canine Infectious Respiratory Disease Complex
por: Thieulent, Côme J., et al.
Publicado: (2023)

Digital Microfluidic Multiplex RT-qPCR for SARS-CoV-2 Detection and Variants Discrimination
por: Ho, Kuan-Lun, et al.
Publicado: (2023)

TaqMan-probe-based multiplex real-time RT-qPCR for simultaneous detection of GoAstV, GPV, and GoCV
por: Yu, Ju, et al.
Publicado: (2022)

Multiplex Real-Time qPCR Assay for Simultaneous and Sensitive Detection of Phytoplasmas in Sesame Plants and Insect Vectors
por: Ikten, Cengiz, et al.
Publicado: (2016)

Development of a Multiplex RT-PCR Assay for Simultaneous Detection of Four Potential Zoonotic Swine RNA Viruses
por: Werid, Gebremeskel Mamu, et al.
Publicado: (2022)

EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR
por: Fout, G. Shay, et al.
Publicado: (2016)

Multiplex qPCR for Detection and Absolute Quantification of Malaria
por: Kamau, Edwin, et al.
Publicado: (2013)

Simultaneous quantification of the most common and proteolytic Pseudomonas species in raw milk by multiplex qPCR
por: Maier, Christopher, et al.
Publicado: (2021)

Algorithm Optimization in Methylation Detection with Multiple RT-qPCR
por: Song, Lele, et al.
Publicado: (2016)

Improving the standardization of mRNA measurement by RT-qPCR
por: Sanders, Rebecca, et al.
Publicado: (2018)

COVID-19 diagnosis by RT-qPCR in alternative specimens
por: Gonçalves, Cássia Cristina Alves, et al.
Publicado: (2021)

Rapid and accurate quantification of isomiRs by RT-qPCR
por: Franco, Sandra, et al.
Publicado: (2022)

IGI-LuNER: single-well multiplexed RT-qPCR test for SARS-CoV-2
por: Stahl, Elizabeth C., et al.
Publicado: (2020)

Confirming Multiplex RT-qPCR Use in COVID-19 with Next-Generation Sequencing: Strategies for Epidemiological Advantage
por: Carpenter, Rob E., et al.
Publicado: (2022)

Performance of a novel melting curve-based qPCR assay for malaria parasites in routine clinical practice in non-endemic setting
por: van Bergen, Kim J. M., et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_ffhhi2iuolqupjfirn6lps72ii