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HMGB1 Promotes Intraoral Palatal Wound Healing through RAGE-Dependent Mechanisms

High mobility group box 1 (HMGB1) is tightly connected to the process of tissue organization upon tissue injury. Here we show that HMGB1 controls epithelium and connective tissue regeneration both in vivo and in vitro during palatal wound healing. Heterozygous HMGB1 (Hmgb1(+/−)) mice and Wild-type (...

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Autores principales: Tancharoen, Salunya, Gando, Satoshi, Binita, Shrestha, Nagasato, Tomoka, Kikuchi, Kiyoshi, Nawa, Yuko, Dararat, Pornpen, Yamamoto, Mika, Narkpinit, Somphong, Maruyama, Ikuro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5133955/
https://www.ncbi.nlm.nih.gov/pubmed/27886093
http://dx.doi.org/10.3390/ijms17111961
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author Tancharoen, Salunya
Gando, Satoshi
Binita, Shrestha
Nagasato, Tomoka
Kikuchi, Kiyoshi
Nawa, Yuko
Dararat, Pornpen
Yamamoto, Mika
Narkpinit, Somphong
Maruyama, Ikuro
author_facet Tancharoen, Salunya
Gando, Satoshi
Binita, Shrestha
Nagasato, Tomoka
Kikuchi, Kiyoshi
Nawa, Yuko
Dararat, Pornpen
Yamamoto, Mika
Narkpinit, Somphong
Maruyama, Ikuro
author_sort Tancharoen, Salunya
collection PubMed
description High mobility group box 1 (HMGB1) is tightly connected to the process of tissue organization upon tissue injury. Here we show that HMGB1 controls epithelium and connective tissue regeneration both in vivo and in vitro during palatal wound healing. Heterozygous HMGB1 (Hmgb1(+/−)) mice and Wild-type (WT) mice were subjected to palatal injury. Maxillary tissues were stained with Mallory Azan or immunostained with anti-HMGB1, anti-proliferating cell nuclear antigen (PCNA), anti-nuclear factor-κB (NF-κB) p50 and anti-vascular endothelial growth factor (VEGF) antibodies. Palatal gingival explants were cultured with recombinant HMGB1 (rHMGB1) co-treated with siRNA targeting receptor for advanced glycation end products (RAGEs) for cell migration and PCNA expression analysis. Measurement of the wound area showed differences between Hmgb1(+/−) and WT mice on Day 3 after wounding. Mallory Azan staining showed densely packed of collagen fibers in WT mice, whereas in Hmgb1(+/−) mice weave-like pattern of low density collagen bundles were present. At three and seven days post-surgery, PCNA, NF-κB p50 and VEGF positive keratinocytes of WT mice were greater than that of Hmgb1(+/−) mice. Knockdown of RAGE prevents the effect of rHMGB1-induced cell migration and PCNA expression in gingival cell cultures. The data suggest that HMGB1/RAGE axis has crucial roles in palatal wound healing.
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spelling pubmed-51339552016-12-12 HMGB1 Promotes Intraoral Palatal Wound Healing through RAGE-Dependent Mechanisms Tancharoen, Salunya Gando, Satoshi Binita, Shrestha Nagasato, Tomoka Kikuchi, Kiyoshi Nawa, Yuko Dararat, Pornpen Yamamoto, Mika Narkpinit, Somphong Maruyama, Ikuro Int J Mol Sci Article High mobility group box 1 (HMGB1) is tightly connected to the process of tissue organization upon tissue injury. Here we show that HMGB1 controls epithelium and connective tissue regeneration both in vivo and in vitro during palatal wound healing. Heterozygous HMGB1 (Hmgb1(+/−)) mice and Wild-type (WT) mice were subjected to palatal injury. Maxillary tissues were stained with Mallory Azan or immunostained with anti-HMGB1, anti-proliferating cell nuclear antigen (PCNA), anti-nuclear factor-κB (NF-κB) p50 and anti-vascular endothelial growth factor (VEGF) antibodies. Palatal gingival explants were cultured with recombinant HMGB1 (rHMGB1) co-treated with siRNA targeting receptor for advanced glycation end products (RAGEs) for cell migration and PCNA expression analysis. Measurement of the wound area showed differences between Hmgb1(+/−) and WT mice on Day 3 after wounding. Mallory Azan staining showed densely packed of collagen fibers in WT mice, whereas in Hmgb1(+/−) mice weave-like pattern of low density collagen bundles were present. At three and seven days post-surgery, PCNA, NF-κB p50 and VEGF positive keratinocytes of WT mice were greater than that of Hmgb1(+/−) mice. Knockdown of RAGE prevents the effect of rHMGB1-induced cell migration and PCNA expression in gingival cell cultures. The data suggest that HMGB1/RAGE axis has crucial roles in palatal wound healing. MDPI 2016-11-23 /pmc/articles/PMC5133955/ /pubmed/27886093 http://dx.doi.org/10.3390/ijms17111961 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tancharoen, Salunya
Gando, Satoshi
Binita, Shrestha
Nagasato, Tomoka
Kikuchi, Kiyoshi
Nawa, Yuko
Dararat, Pornpen
Yamamoto, Mika
Narkpinit, Somphong
Maruyama, Ikuro
HMGB1 Promotes Intraoral Palatal Wound Healing through RAGE-Dependent Mechanisms
title HMGB1 Promotes Intraoral Palatal Wound Healing through RAGE-Dependent Mechanisms
title_full HMGB1 Promotes Intraoral Palatal Wound Healing through RAGE-Dependent Mechanisms
title_fullStr HMGB1 Promotes Intraoral Palatal Wound Healing through RAGE-Dependent Mechanisms
title_full_unstemmed HMGB1 Promotes Intraoral Palatal Wound Healing through RAGE-Dependent Mechanisms
title_short HMGB1 Promotes Intraoral Palatal Wound Healing through RAGE-Dependent Mechanisms
title_sort hmgb1 promotes intraoral palatal wound healing through rage-dependent mechanisms
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5133955/
https://www.ncbi.nlm.nih.gov/pubmed/27886093
http://dx.doi.org/10.3390/ijms17111961
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