Cargando…

Discrete adipose-derived stem cell subpopulations may display differential functionality after in vitro expansion despite convergence to a common phenotype distribution

BACKGROUND: Complex immunophenotypic repertoires defining discrete adipose-derived stem cell (ASC) subpopulations may hold a key toward identifying predictors of clinical utility. To this end, we sorted out of the freshly established ASCs four subpopulations (SPs) according to a specific pattern of...

Descripción completa

Detalles Bibliográficos
Autores principales: Nielsen, Frederik Mølgaard, Riis, Simone Elkjær, Andersen, Jens Isak, Lesage, Raphaëlle, Fink, Trine, Pennisi, Cristian Pablo, Zachar, Vladimir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5134234/
https://www.ncbi.nlm.nih.gov/pubmed/27906060
http://dx.doi.org/10.1186/s13287-016-0435-8
Descripción
Sumario:BACKGROUND: Complex immunophenotypic repertoires defining discrete adipose-derived stem cell (ASC) subpopulations may hold a key toward identifying predictors of clinical utility. To this end, we sorted out of the freshly established ASCs four subpopulations (SPs) according to a specific pattern of co-expression of six surface markers, the CD34, CD73, CD90, CD105, CD146, and CD271, using polychromatic flow cytometry. METHOD: Using flow cytometry-associated cell sorting and analysis, gating parameters were set to select for a CD73(+)CD90(+)CD105(+) phenotype plus one of the four following combinations, CD34(−)CD146(−)CD271(−) (SP1), CD34(−)CD146(+)CD271(−) (SP2), CD34(+)CD146(+)CD271(−) (SP3), and CD34(−)CD146(+)CD271(+) (SP4). The SPs were expanded 700- to 1000-fold, and their surface repertoire, trilineage differentiation, and clonogenic potential, and the capacity to support wound healing were assayed. RESULTS: Upon culturing, the co-expression of major epitopes, the CD73, CD90, and CD105 was maintained, while regarding the minor markers, all SPs reverted to resemble the pre-sorted population with CD34(−)CD146(−)CD271(−) and CD34(−)CD146(+)CD271(−) representing the most prevalent combinations, followed by less frequent CD34(+)CD146(−)CD271(−) and CD34(+)CD146(+)CD271(−) variants. There was no difference in the efficiency of adipo-, osteo-, or chondrogenesis by cytochemistry and real-time RT-PCR or the CFU capacity between the individual SPs, however, the SP2(CD73+90+105+34-146+271-) outperformed others in terms of wound healing. CONCLUSIONS: Our study shows that ASCs upon culturing inherently maintain a stable distribution of immunophenotype variants, which may potentially disguise specific functional properties of particular downstream lines. Furthermore, the outlined approach suggests a paradigm whereby discrete subpopulations could be identified to provide for a therapeutically most relevant cell product. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13287-016-0435-8) contains supplementary material, which is available to authorized users.