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Development of a fast and easy method for Escherichia coli genome editing with CRISPR/Cas9
BACKGROUND: Microbial genome editing is a powerful tool to modify chromosome in way of deletion, insertion or replacement, which is one of the most important techniques in metabolic engineering research. The emergence of CRISPR/Cas9 technique inspires various genomic editing methods. RESULTS: In thi...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5134288/ https://www.ncbi.nlm.nih.gov/pubmed/27908280 http://dx.doi.org/10.1186/s12934-016-0605-5 |