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A Novel Application for Low Frequency Electrochemical Impedance Spectroscopy as an Online Process Monitoring Tool for Viable Cell Concentrations

New approaches in process monitoring during industrial fermentations are not only limited to classical pH, dO(2) and offgas analysis, but use different in situ and online sensors based on different physical principles to determine biomass, product quality, lysis and far more. One of the very importa...

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Autores principales: Slouka, Christoph, Wurm, David J., Brunauer, Georg, Welzl-Wachter, Andreas, Spadiut, Oliver, Fleig, Jürgen, Herwig, Christoph
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5134559/
https://www.ncbi.nlm.nih.gov/pubmed/27845720
http://dx.doi.org/10.3390/s16111900
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author Slouka, Christoph
Wurm, David J.
Brunauer, Georg
Welzl-Wachter, Andreas
Spadiut, Oliver
Fleig, Jürgen
Herwig, Christoph
author_facet Slouka, Christoph
Wurm, David J.
Brunauer, Georg
Welzl-Wachter, Andreas
Spadiut, Oliver
Fleig, Jürgen
Herwig, Christoph
author_sort Slouka, Christoph
collection PubMed
description New approaches in process monitoring during industrial fermentations are not only limited to classical pH, dO(2) and offgas analysis, but use different in situ and online sensors based on different physical principles to determine biomass, product quality, lysis and far more. One of the very important approaches is the in situ accessibility of viable cell concentration (VCC). This knowledge provides increased efficiency in monitoring and controlling strategies during cultivations. Electrochemical impedance spectroscopy—EIS—is used to monitor biomass in a fermentation of E. coli BL21(DE3), producing a recombinant protein using a fed batch-based approach. Increases in the double layer capacitance (C(dl)), determined at frequencies below 1 kHz, are proportional to the increase of biomass in the batch and fed batch phase, monitored in offline and online modes for different cultivations. A good correlation of C(dl) with cell density is found and in order to get an appropriate verification of this method, different state-of-the-art biomass measurements are performed and compared. Since measurements in this frequency range are largely determined by the double layer region between the electrode and media, rather minor interferences with process parameters (aeration, stirring) are to be expected. It is shown that impedance spectroscopy at low frequencies is a powerful tool for cultivation monitoring.
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spelling pubmed-51345592017-01-03 A Novel Application for Low Frequency Electrochemical Impedance Spectroscopy as an Online Process Monitoring Tool for Viable Cell Concentrations Slouka, Christoph Wurm, David J. Brunauer, Georg Welzl-Wachter, Andreas Spadiut, Oliver Fleig, Jürgen Herwig, Christoph Sensors (Basel) Article New approaches in process monitoring during industrial fermentations are not only limited to classical pH, dO(2) and offgas analysis, but use different in situ and online sensors based on different physical principles to determine biomass, product quality, lysis and far more. One of the very important approaches is the in situ accessibility of viable cell concentration (VCC). This knowledge provides increased efficiency in monitoring and controlling strategies during cultivations. Electrochemical impedance spectroscopy—EIS—is used to monitor biomass in a fermentation of E. coli BL21(DE3), producing a recombinant protein using a fed batch-based approach. Increases in the double layer capacitance (C(dl)), determined at frequencies below 1 kHz, are proportional to the increase of biomass in the batch and fed batch phase, monitored in offline and online modes for different cultivations. A good correlation of C(dl) with cell density is found and in order to get an appropriate verification of this method, different state-of-the-art biomass measurements are performed and compared. Since measurements in this frequency range are largely determined by the double layer region between the electrode and media, rather minor interferences with process parameters (aeration, stirring) are to be expected. It is shown that impedance spectroscopy at low frequencies is a powerful tool for cultivation monitoring. MDPI 2016-11-11 /pmc/articles/PMC5134559/ /pubmed/27845720 http://dx.doi.org/10.3390/s16111900 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Slouka, Christoph
Wurm, David J.
Brunauer, Georg
Welzl-Wachter, Andreas
Spadiut, Oliver
Fleig, Jürgen
Herwig, Christoph
A Novel Application for Low Frequency Electrochemical Impedance Spectroscopy as an Online Process Monitoring Tool for Viable Cell Concentrations
title A Novel Application for Low Frequency Electrochemical Impedance Spectroscopy as an Online Process Monitoring Tool for Viable Cell Concentrations
title_full A Novel Application for Low Frequency Electrochemical Impedance Spectroscopy as an Online Process Monitoring Tool for Viable Cell Concentrations
title_fullStr A Novel Application for Low Frequency Electrochemical Impedance Spectroscopy as an Online Process Monitoring Tool for Viable Cell Concentrations
title_full_unstemmed A Novel Application for Low Frequency Electrochemical Impedance Spectroscopy as an Online Process Monitoring Tool for Viable Cell Concentrations
title_short A Novel Application for Low Frequency Electrochemical Impedance Spectroscopy as an Online Process Monitoring Tool for Viable Cell Concentrations
title_sort novel application for low frequency electrochemical impedance spectroscopy as an online process monitoring tool for viable cell concentrations
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5134559/
https://www.ncbi.nlm.nih.gov/pubmed/27845720
http://dx.doi.org/10.3390/s16111900
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