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Genotypic distribution of HHV-8 in AIDS individuals without and with Kaposi sarcoma: Is genotype B associated with better prognosis of AIDS-KS?
AIDS-associated Kaposi's sarcoma (AIDS-KS) caused by human herpes virus 8 (HHV-8) is the most severe and resistant form of KS tumor. Our aim was to verify whether there is an association between HHV-8 variability and development of AIDS-KS in Brazil by comparing the HHV-8 variability between in...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Wolters Kluwer Health
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5134807/ https://www.ncbi.nlm.nih.gov/pubmed/27902590 http://dx.doi.org/10.1097/MD.0000000000005291 |
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author | Tozetto-Mendoza, Tania Regina Ibrahim, Karim Yaqub Tateno, Adriana Fumie de Oliveira, Cristiane Mendes Sumita, Laura Massami Sanchez, Maria Carmem Arroyo Luna, Expedito José Pierrotti, Ligia Camara Drexler, Jan Felix Braz-Silva, Paulo Henrique Pannuti, Claudio Sérgio Romano, Camila Malta |
author_facet | Tozetto-Mendoza, Tania Regina Ibrahim, Karim Yaqub Tateno, Adriana Fumie de Oliveira, Cristiane Mendes Sumita, Laura Massami Sanchez, Maria Carmem Arroyo Luna, Expedito José Pierrotti, Ligia Camara Drexler, Jan Felix Braz-Silva, Paulo Henrique Pannuti, Claudio Sérgio Romano, Camila Malta |
author_sort | Tozetto-Mendoza, Tania Regina |
collection | PubMed |
description | AIDS-associated Kaposi's sarcoma (AIDS-KS) caused by human herpes virus 8 (HHV-8) is the most severe and resistant form of KS tumor. Our aim was to verify whether there is an association between HHV-8 variability and development of AIDS-KS in Brazil by comparing the HHV-8 variability between individuals without and with KS. Saliva samples and blood, when available, were analyzed by polymerase chain reaction (PCR) techniques for detection of the fragments of ORF K1 of HHV-8, which were then genotyped and analyzed regarding the genetic variability. Our study described 106 positive cases for HHV-8 in the saliva from 751 AIDS patients without previous KS. In addition, we performed a phylogenetic analysis of HHV-8 in 34 of the 106 AIDS patients without KS and in 33 of the 37 patients with active KS. The distribution of HHV-8 genotypes A, B, C, and F in AIDS individuals was indistinguishable by comparing non-KS and KS groups, as well as regarding ethnicity. Considering the KS group, genotype B was associated with better prognosis of KS tumor. Interestingly, we found a particular profile of diversity within clade C and 2 recombinant patterns of HHV-8 in the saliva of AIDS individuals without KS. We emphasize the need to achieve standard genotyping protocol for ORF K1 amplification, thus allowing for substantial detection of HHV-8 variants. Our findings can shed light on the role of HHV-8 variability in the pathogenesis of AIDS-KS. |
format | Online Article Text |
id | pubmed-5134807 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Wolters Kluwer Health |
record_format | MEDLINE/PubMed |
spelling | pubmed-51348072016-12-22 Genotypic distribution of HHV-8 in AIDS individuals without and with Kaposi sarcoma: Is genotype B associated with better prognosis of AIDS-KS? Tozetto-Mendoza, Tania Regina Ibrahim, Karim Yaqub Tateno, Adriana Fumie de Oliveira, Cristiane Mendes Sumita, Laura Massami Sanchez, Maria Carmem Arroyo Luna, Expedito José Pierrotti, Ligia Camara Drexler, Jan Felix Braz-Silva, Paulo Henrique Pannuti, Claudio Sérgio Romano, Camila Malta Medicine (Baltimore) 4850 AIDS-associated Kaposi's sarcoma (AIDS-KS) caused by human herpes virus 8 (HHV-8) is the most severe and resistant form of KS tumor. Our aim was to verify whether there is an association between HHV-8 variability and development of AIDS-KS in Brazil by comparing the HHV-8 variability between individuals without and with KS. Saliva samples and blood, when available, were analyzed by polymerase chain reaction (PCR) techniques for detection of the fragments of ORF K1 of HHV-8, which were then genotyped and analyzed regarding the genetic variability. Our study described 106 positive cases for HHV-8 in the saliva from 751 AIDS patients without previous KS. In addition, we performed a phylogenetic analysis of HHV-8 in 34 of the 106 AIDS patients without KS and in 33 of the 37 patients with active KS. The distribution of HHV-8 genotypes A, B, C, and F in AIDS individuals was indistinguishable by comparing non-KS and KS groups, as well as regarding ethnicity. Considering the KS group, genotype B was associated with better prognosis of KS tumor. Interestingly, we found a particular profile of diversity within clade C and 2 recombinant patterns of HHV-8 in the saliva of AIDS individuals without KS. We emphasize the need to achieve standard genotyping protocol for ORF K1 amplification, thus allowing for substantial detection of HHV-8 variants. Our findings can shed light on the role of HHV-8 variability in the pathogenesis of AIDS-KS. Wolters Kluwer Health 2016-12-02 /pmc/articles/PMC5134807/ /pubmed/27902590 http://dx.doi.org/10.1097/MD.0000000000005291 Text en Copyright © 2016 the Author(s). Published by Wolters Kluwer Health, Inc. All rights reserved. http://creativecommons.org/licenses/by/4.0 This is an open access article distributed under the Creative Commons Attribution License 4.0 (CCBY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. http://creativecommons.org/licenses/by/4.0 |
spellingShingle | 4850 Tozetto-Mendoza, Tania Regina Ibrahim, Karim Yaqub Tateno, Adriana Fumie de Oliveira, Cristiane Mendes Sumita, Laura Massami Sanchez, Maria Carmem Arroyo Luna, Expedito José Pierrotti, Ligia Camara Drexler, Jan Felix Braz-Silva, Paulo Henrique Pannuti, Claudio Sérgio Romano, Camila Malta Genotypic distribution of HHV-8 in AIDS individuals without and with Kaposi sarcoma: Is genotype B associated with better prognosis of AIDS-KS? |
title | Genotypic distribution of HHV-8 in AIDS individuals without and with Kaposi sarcoma: Is genotype B associated with better prognosis of AIDS-KS? |
title_full | Genotypic distribution of HHV-8 in AIDS individuals without and with Kaposi sarcoma: Is genotype B associated with better prognosis of AIDS-KS? |
title_fullStr | Genotypic distribution of HHV-8 in AIDS individuals without and with Kaposi sarcoma: Is genotype B associated with better prognosis of AIDS-KS? |
title_full_unstemmed | Genotypic distribution of HHV-8 in AIDS individuals without and with Kaposi sarcoma: Is genotype B associated with better prognosis of AIDS-KS? |
title_short | Genotypic distribution of HHV-8 in AIDS individuals without and with Kaposi sarcoma: Is genotype B associated with better prognosis of AIDS-KS? |
title_sort | genotypic distribution of hhv-8 in aids individuals without and with kaposi sarcoma: is genotype b associated with better prognosis of aids-ks? |
topic | 4850 |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5134807/ https://www.ncbi.nlm.nih.gov/pubmed/27902590 http://dx.doi.org/10.1097/MD.0000000000005291 |
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