Cargando…

Do we know whether potential G-quadruplexes actually form in long functional RNA molecules?

The roles of deoxyribonucleic acid (DNA) G-quadruplex structures in gene expression and telomere maintenance have been well characterized. Recent results suggest that such structures could also play pivotal roles in ribonucleic acid (RNA) biology, such as splicing or translation regulation. However,...

Descripción completa

Detalles Bibliográficos
Autores principales: Weldon, Carika, Eperon, Ian C., Dominguez, Cyril
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2016
Materias:
RNA
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5135001/
https://www.ncbi.nlm.nih.gov/pubmed/27913687
http://dx.doi.org/10.1042/BST20160109
_version_ 1782471554342322176
author Weldon, Carika
Eperon, Ian C.
Dominguez, Cyril
author_facet Weldon, Carika
Eperon, Ian C.
Dominguez, Cyril
author_sort Weldon, Carika
collection PubMed
description The roles of deoxyribonucleic acid (DNA) G-quadruplex structures in gene expression and telomere maintenance have been well characterized. Recent results suggest that such structures could also play pivotal roles in ribonucleic acid (RNA) biology, such as splicing or translation regulation. However, it has been difficult to show that RNA G-quadruplexes (G4s) exist in specific long RNA sequences, such as precursor messenger RNA, in a functional or cellular context. Most current methods for identifying G4s involve the use of short, purified RNA sequences in vitro, in the absence of competition with secondary structures or protein binding. Therefore, novel methods need to be developed to allow the characterization of G4s in long functional RNAs and in a cellular context. This need has in part been met by our recent development of a method based on a comparison of RNA and 7-deaza-RNA that provides a test for identifying RNA G4s in such conditions.
format Online
Article
Text
id pubmed-5135001
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Portland Press Ltd.
record_format MEDLINE/PubMed
spelling pubmed-51350012016-12-16 Do we know whether potential G-quadruplexes actually form in long functional RNA molecules? Weldon, Carika Eperon, Ian C. Dominguez, Cyril Biochem Soc Trans RNA The roles of deoxyribonucleic acid (DNA) G-quadruplex structures in gene expression and telomere maintenance have been well characterized. Recent results suggest that such structures could also play pivotal roles in ribonucleic acid (RNA) biology, such as splicing or translation regulation. However, it has been difficult to show that RNA G-quadruplexes (G4s) exist in specific long RNA sequences, such as precursor messenger RNA, in a functional or cellular context. Most current methods for identifying G4s involve the use of short, purified RNA sequences in vitro, in the absence of competition with secondary structures or protein binding. Therefore, novel methods need to be developed to allow the characterization of G4s in long functional RNAs and in a cellular context. This need has in part been met by our recent development of a method based on a comparison of RNA and 7-deaza-RNA that provides a test for identifying RNA G4s in such conditions. Portland Press Ltd. 2016-12-15 2016-12-02 /pmc/articles/PMC5135001/ /pubmed/27913687 http://dx.doi.org/10.1042/BST20160109 Text en © 2016 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (https://creativecommons.org/licenses/by/4.0) .
spellingShingle RNA
Weldon, Carika
Eperon, Ian C.
Dominguez, Cyril
Do we know whether potential G-quadruplexes actually form in long functional RNA molecules?
title Do we know whether potential G-quadruplexes actually form in long functional RNA molecules?
title_full Do we know whether potential G-quadruplexes actually form in long functional RNA molecules?
title_fullStr Do we know whether potential G-quadruplexes actually form in long functional RNA molecules?
title_full_unstemmed Do we know whether potential G-quadruplexes actually form in long functional RNA molecules?
title_short Do we know whether potential G-quadruplexes actually form in long functional RNA molecules?
title_sort do we know whether potential g-quadruplexes actually form in long functional rna molecules?
topic RNA
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5135001/
https://www.ncbi.nlm.nih.gov/pubmed/27913687
http://dx.doi.org/10.1042/BST20160109
work_keys_str_mv AT weldoncarika doweknowwhetherpotentialgquadruplexesactuallyforminlongfunctionalrnamolecules
AT eperonianc doweknowwhetherpotentialgquadruplexesactuallyforminlongfunctionalrnamolecules
AT dominguezcyril doweknowwhetherpotentialgquadruplexesactuallyforminlongfunctionalrnamolecules