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A combined chloroplast atpB–rbcL and trnL-F phylogeny unveils the ancestry of balsams (Impatiens spp.) in the Western Ghats of India

Only a few Impatiens spp. from South India (one of the five centers of diversity for Impatiens species) were included in the published datum of molecular phylogeny of the family Balsaminaceae. The present investigation is a novel attempt to reveal the phylogenetic association of Impatiens species of...

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Autores principales: Shajitha, P. P., Dhanesh, N. R., Ebin, P. J., Laly, Joseph, Aneesha, Devassy, Reshma, John, Augustine, Jomy, Linu, Mathew
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5135705/
https://www.ncbi.nlm.nih.gov/pubmed/28330330
http://dx.doi.org/10.1007/s13205-016-0574-8
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author Shajitha, P. P.
Dhanesh, N. R.
Ebin, P. J.
Laly, Joseph
Aneesha, Devassy
Reshma, John
Augustine, Jomy
Linu, Mathew
author_facet Shajitha, P. P.
Dhanesh, N. R.
Ebin, P. J.
Laly, Joseph
Aneesha, Devassy
Reshma, John
Augustine, Jomy
Linu, Mathew
author_sort Shajitha, P. P.
collection PubMed
description Only a few Impatiens spp. from South India (one of the five centers of diversity for Impatiens species) were included in the published datum of molecular phylogeny of the family Balsaminaceae. The present investigation is a novel attempt to reveal the phylogenetic association of Impatiens species of South India, by placing them in the global phylogeny of Impatiens based on a combined analysis of two chloroplast genes. Thirty species of genus Impatiens were collected from different locations of South India. Total genomic DNA was extracted from fresh plant leaf, and polymerase chain reaction was carried out using atpB–rbcL and trnL-F intergenic spacer-specific forward and reverse primers. Thirteen sequences of Impatiens species from three centers of diversity were obtained from GenBank for reconstructing the evolutionary relationships within the genus Impatiens. Bayesian inference analysis was carried out in MrBayes v.3.2.2. This analysis supported Southeast Asia as the ancestral place of origin of extant Impatiens species. Molecular phylogeny of South Indian Impatiens spp. based on combined chloroplast sequences showed the same association as that of morphological taxonomy. Sections Scapigerae, Tomentosae, Sub-Umbellatae, and Racemosae showed Southeast Asian relationship, while sections Annuae and Microsepalae showed African affinity. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s13205-016-0574-8) contains supplementary material, which is available to authorized users.
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spelling pubmed-51357052016-12-19 A combined chloroplast atpB–rbcL and trnL-F phylogeny unveils the ancestry of balsams (Impatiens spp.) in the Western Ghats of India Shajitha, P. P. Dhanesh, N. R. Ebin, P. J. Laly, Joseph Aneesha, Devassy Reshma, John Augustine, Jomy Linu, Mathew 3 Biotech Short Reports Only a few Impatiens spp. from South India (one of the five centers of diversity for Impatiens species) were included in the published datum of molecular phylogeny of the family Balsaminaceae. The present investigation is a novel attempt to reveal the phylogenetic association of Impatiens species of South India, by placing them in the global phylogeny of Impatiens based on a combined analysis of two chloroplast genes. Thirty species of genus Impatiens were collected from different locations of South India. Total genomic DNA was extracted from fresh plant leaf, and polymerase chain reaction was carried out using atpB–rbcL and trnL-F intergenic spacer-specific forward and reverse primers. Thirteen sequences of Impatiens species from three centers of diversity were obtained from GenBank for reconstructing the evolutionary relationships within the genus Impatiens. Bayesian inference analysis was carried out in MrBayes v.3.2.2. This analysis supported Southeast Asia as the ancestral place of origin of extant Impatiens species. Molecular phylogeny of South Indian Impatiens spp. based on combined chloroplast sequences showed the same association as that of morphological taxonomy. Sections Scapigerae, Tomentosae, Sub-Umbellatae, and Racemosae showed Southeast Asian relationship, while sections Annuae and Microsepalae showed African affinity. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s13205-016-0574-8) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2016-12-02 2016-12 /pmc/articles/PMC5135705/ /pubmed/28330330 http://dx.doi.org/10.1007/s13205-016-0574-8 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Short Reports
Shajitha, P. P.
Dhanesh, N. R.
Ebin, P. J.
Laly, Joseph
Aneesha, Devassy
Reshma, John
Augustine, Jomy
Linu, Mathew
A combined chloroplast atpB–rbcL and trnL-F phylogeny unveils the ancestry of balsams (Impatiens spp.) in the Western Ghats of India
title A combined chloroplast atpB–rbcL and trnL-F phylogeny unveils the ancestry of balsams (Impatiens spp.) in the Western Ghats of India
title_full A combined chloroplast atpB–rbcL and trnL-F phylogeny unveils the ancestry of balsams (Impatiens spp.) in the Western Ghats of India
title_fullStr A combined chloroplast atpB–rbcL and trnL-F phylogeny unveils the ancestry of balsams (Impatiens spp.) in the Western Ghats of India
title_full_unstemmed A combined chloroplast atpB–rbcL and trnL-F phylogeny unveils the ancestry of balsams (Impatiens spp.) in the Western Ghats of India
title_short A combined chloroplast atpB–rbcL and trnL-F phylogeny unveils the ancestry of balsams (Impatiens spp.) in the Western Ghats of India
title_sort combined chloroplast atpb–rbcl and trnl-f phylogeny unveils the ancestry of balsams (impatiens spp.) in the western ghats of india
topic Short Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5135705/
https://www.ncbi.nlm.nih.gov/pubmed/28330330
http://dx.doi.org/10.1007/s13205-016-0574-8
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