Measurement of amyloid formation by turbidity assay—seeing through the cloud

Detection of amyloid growth is commonly carried out by measurement of solution turbidity, a low-cost assay procedure based on the intrinsic light scattering properties of the protein aggregate. Here, we review the biophysical chemistry associated with the turbidimetric assay methodology, exploring t...

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Detalles Bibliográficos
Autores principales: Zhao, Ran, So, Masatomo, Maat, Hendrik, Ray, Nicholas J., Arisaka, Fumio, Goto, Yuji, Carver, John A., Hall, Damien
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2016
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5135725/
https://www.ncbi.nlm.nih.gov/pubmed/28003859
http://dx.doi.org/10.1007/s12551-016-0233-7
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author Zhao, Ran
So, Masatomo
Maat, Hendrik
Ray, Nicholas J.
Arisaka, Fumio
Goto, Yuji
Carver, John A.
Hall, Damien
author_facet Zhao, Ran
So, Masatomo
Maat, Hendrik
Ray, Nicholas J.
Arisaka, Fumio
Goto, Yuji
Carver, John A.
Hall, Damien
author_sort Zhao, Ran
collection PubMed
description Detection of amyloid growth is commonly carried out by measurement of solution turbidity, a low-cost assay procedure based on the intrinsic light scattering properties of the protein aggregate. Here, we review the biophysical chemistry associated with the turbidimetric assay methodology, exploring the reviewed literature using a series of pedagogical kinetic simulations. In turn, these simulations are used to interrogate the literature concerned with in vitro drug screening and the assessment of amyloid aggregation mechanisms.
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spelling pubmed-51357252016-12-19 Measurement of amyloid formation by turbidity assay—seeing through the cloud Zhao, Ran So, Masatomo Maat, Hendrik Ray, Nicholas J. Arisaka, Fumio Goto, Yuji Carver, John A. Hall, Damien Biophys Rev Review Detection of amyloid growth is commonly carried out by measurement of solution turbidity, a low-cost assay procedure based on the intrinsic light scattering properties of the protein aggregate. Here, we review the biophysical chemistry associated with the turbidimetric assay methodology, exploring the reviewed literature using a series of pedagogical kinetic simulations. In turn, these simulations are used to interrogate the literature concerned with in vitro drug screening and the assessment of amyloid aggregation mechanisms. Springer Berlin Heidelberg 2016-11-23 /pmc/articles/PMC5135725/ /pubmed/28003859 http://dx.doi.org/10.1007/s12551-016-0233-7 Text en © The Author(s) 2016 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Review
Zhao, Ran
So, Masatomo
Maat, Hendrik
Ray, Nicholas J.
Arisaka, Fumio
Goto, Yuji
Carver, John A.
Hall, Damien
Measurement of amyloid formation by turbidity assay—seeing through the cloud
title Measurement of amyloid formation by turbidity assay—seeing through the cloud
title_full Measurement of amyloid formation by turbidity assay—seeing through the cloud
title_fullStr Measurement of amyloid formation by turbidity assay—seeing through the cloud
title_full_unstemmed Measurement of amyloid formation by turbidity assay—seeing through the cloud
title_short Measurement of amyloid formation by turbidity assay—seeing through the cloud
title_sort measurement of amyloid formation by turbidity assay—seeing through the cloud
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5135725/
https://www.ncbi.nlm.nih.gov/pubmed/28003859
http://dx.doi.org/10.1007/s12551-016-0233-7
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