Analyzing structure–function relationships of artificial and cancer-associated PARP1 variants by reconstituting TALEN-generated HeLa PARP1 knock-out cells

Genotoxic stress activates PARP1, resulting in the post-translational modification of proteins with poly(ADP-ribose) (PAR). We genetically deleted PARP1 in one of the most widely used human cell systems, i.e. HeLa cells, via TALEN-mediated gene targeting. After comprehensive characterization of thes...

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Detalles Bibliográficos
Autores principales: Rank, Lisa, Veith, Sebastian, Gwosch, Eva C., Demgenski, Janine, Ganz, Magdalena, Jongmans, Marjolijn C., Vogel, Christopher, Fischbach, Arthur, Buerger, Stefanie, Fischer, Jan M.F., Zubel, Tabea, Stier, Anna, Renner, Christina, Schmalz, Michael, Beneke, Sascha, Groettrup, Marcus, Kuiper, Roland P., Bürkle, Alexander, Ferrando-May, Elisa, Mangerich, Aswin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2016
Materias:
Nucleic Acid Enzymes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5137445/
https://www.ncbi.nlm.nih.gov/pubmed/27694308
http://dx.doi.org/10.1093/nar/gkw859

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