Cargando…

Insightful directed evolution of Escherichia coli quorum sensing promoter region of the lsrACDBFG operon: a tool for synthetic biology systems and protein expression

Quorum sensing (QS) regulates many natural phenotypes (e.q. virulence, biofilm formation, antibiotic resistance), and its components, when incorporated into synthetic genetic circuits, enable user-directed phenotypes. We created a library of Escherichia coli lsr operon promoters using error-prone PC...

Descripción completa

Detalles Bibliográficos
Autores principales:	Hauk, Pricila, Stephens, Kristina, Mckay, Ryan, Virgile, Chelsea Ryan, Ueda, Hana, Ostermeier, Marc, Ryu, Kyoung-Seok, Sintim, Herman O., Bentley, William E.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2016
Materias:	Synthetic Biology and Bioengineering
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5137460/ https://www.ncbi.nlm.nih.gov/pubmed/27915294 http://dx.doi.org/10.1093/nar/gkw981

Ejemplares similares

Evidence of link between quorum sensing and sugar metabolism in Escherichia coli revealed via cocrystal structures of LsrK and HPr
por: Ha, Jung-Hye, et al.
Publicado: (2018)

A predictive biophysical model of translational coupling to coordinate and control protein expression in bacterial operons
por: Tian, Tian, et al.
Publicado: (2015)

LsrR Quorum Sensing “Switch” Is Revealed by a Bottom-Up Approach
por: Hooshangi, Sara, et al.
Publicado: (2011)

Developing an endogenous quorum-sensing based CRISPRi circuit for autonomous and tunable dynamic regulation of multiple targets in Streptomyces
por: Tian, Jinzhong, et al.
Publicado: (2020)

Lsr operon is associated with AI-2 transfer and pathogenicity in avian pathogenic Escherichia coli
por: Zuo, Jiakun, et al.
Publicado: (2019)

Modification and Assembly of a Versatile Lactonase for Bacterial Quorum Quenching
por: Rhoads, Melissa K., et al.
Publicado: (2018)

Repurposing endogenous type I CRISPR-Cas systems for programmable gene repression
por: Luo, Michelle L., et al.
Publicado: (2015)

Co-incident insertion enables high efficiency genome engineering in mouse embryonic stem cells
por: Shy, Brian R., et al.
Publicado: (2016)

Multilayered genetic safeguards limit growth of microorganisms to defined environments
por: Gallagher, Ryan R., et al.
Publicado: (2015)

A versatile platform strain for high-fidelity multiplex genome editing
por: Egbert, Robert G, et al.
Publicado: (2019)

Toehold clipping: A mechanism for remote control of DNA strand displacement
por: Faheem, Hiba, et al.
Publicado: (2022)

Targeting Quorum Sensing: High-Throughput Screening to Identify Novel LsrK Inhibitors
por: Gatta, Viviana, et al.
Publicado: (2019)

Bacteria Floc, but Do They Flock? Insights from Population Interaction Models of Quorum Sensing
por: Ueda, Hana, et al.
Publicado: (2019)

Heterodimeric DNA methyltransferases as a platform for creating designer zinc finger methyltransferases for targeted DNA methylation in cells
por: Meister, Glenna E., et al.
Publicado: (2010)

Engineering bacterial motility towards hydrogen-peroxide
por: Virgile, Chelsea, et al.
Publicado: (2018)

Indole and azaindole halogenation catalyzed by the RebH enzyme variant 3-LSR utilizing co-purified E. coli reductase
por: Li, Eunice Hui Yen, et al.
Publicado: (2022)

Can the Natural Diversity of Quorum-Sensing Advance Synthetic Biology?
por: Davis, René Michele, et al.
Publicado: (2015)

BeQuIK (Biosensor Engineered Quorum Induced Killing): designer bacteria for destroying recalcitrant biofilms
por: Riangrungroj, Pinpunya, et al.
Publicado: (2019)

Quorum Sensing Autoinducer-3 Finally Yields to Structural Elucidation
por: Hernandez, Delmis E., et al.
Publicado: (2020)

A universal strategy for regulating mRNA translation in prokaryotic and eukaryotic cells
por: Cao, Jicong, et al.
Publicado: (2015)

Selective inhibition of miR-21 by phage display screened peptide
por: Bose, Debojit, et al.
Publicado: (2015)

A general approach to high-yield biosynthesis of chimeric RNAs bearing various types of functional small RNAs for broad applications
por: Chen, Qiu-Xia, et al.
Publicado: (2015)

A genome-wide analysis of Cas9 binding specificity using ChIP-seq and targeted sequence capture
por: O'Geen, Henriette, et al.
Publicado: (2015)

De novo design of heat-repressible RNA thermosensors in E. coli
por: Hoynes-O'Connor, Allison, et al.
Publicado: (2015)

Tight regulation of plant immune responses by combining promoter and suicide exon elements
por: Gonzalez, Tania L., et al.
Publicado: (2015)

A fungicide-responsive kinase as a tool for synthetic cell fate regulation
por: Furukawa, Kentaro, et al.
Publicado: (2015)

Synthetic biosensors for precise gene control and real-time monitoring of metabolites
por: Rogers, Jameson K., et al.
Publicado: (2015)

Selection-free gene repair after adenoviral vector transduction of designer nucleases: rescue of dystrophin synthesis in DMD muscle cell populations
por: Maggio, Ignazio, et al.
Publicado: (2016)

Locked and proteolysis-based transcription activator-like effector (TALE) regulation
por: Lonzarić, Jan, et al.
Publicado: (2016)

Dealing with the genetic load in bacterial synthetic biology circuits: convergences with the Ohm's law
por: Carbonell-Ballestero, M., et al.
Publicado: (2016)

Algorithmic co-optimization of genetic constructs and growth conditions: application to 6-ACA, a potential nylon-6 precursor
por: Zhou, Hui, et al.
Publicado: (2015)

Reassignment of a rare sense codon to a non-canonical amino acid in Escherichia coli
por: Mukai, Takahito, et al.
Publicado: (2015)

Probing the active site tryptophan of Staphylococcus aureus thioredoxin with an analog
por: Englert, Markus, et al.
Publicado: (2015)

A platform for rapid prototyping of synthetic gene networks in mammalian cells
por: Duportet, Xavier, et al.
Publicado: (2014)

Synergetic regulation of translational reading-frame switch by ligand-responsive RNAs in mammalian cells
por: Hsu, Hsiu-Ting, et al.
Publicado: (2014)

A bottom-up characterization of transfer functions for synthetic biology designs: lessons from enzymology
por: Carbonell-Ballestero, Max, et al.
Publicado: (2014)

CRISPR-based self-cleaving mechanism for controllable gene delivery in human cells
por: Moore, Richard, et al.
Publicado: (2015)

Global coordination in adaptation to gene rewiring
por: Murakami, Yoshie, et al.
Publicado: (2015)

Protein-responsive ribozyme switches in eukaryotic cells
por: Kennedy, Andrew B., et al.
Publicado: (2014)

A split intein T7 RNA polymerase for transcriptional AND-logic
por: Schaerli, Yolanda, et al.
Publicado: (2014)

Cannot write session to /tmp/vufind_sessions/sess_i7s8bmokc9081939daiah5hth2