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Stage-Specific Transcriptome and Proteome Analyses of the Filarial Parasite Onchocerca volvulus and Its Wolbachia Endosymbiont
Onchocerciasis (river blindness) is a neglected tropical disease that has been successfully targeted by mass drug treatment programs in the Americas and small parts of Africa. Achieving the long-term goal of elimination of onchocerciasis, however, requires additional tools, including drugs, vaccines...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Microbiology
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5137501/ https://www.ncbi.nlm.nih.gov/pubmed/27881553 http://dx.doi.org/10.1128/mBio.02028-16 |
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author | Bennuru, Sasisekhar Cotton, James A. Ribeiro, Jose M. C. Grote, Alexandra Harsha, Bhavana Holroyd, Nancy Mhashilkar, Amruta Molina, Douglas M. Randall, Arlo Z. Shandling, Adam D. Unnasch, Thomas R. Ghedin, Elodie Berriman, Matthew Lustigman, Sara Nutman, Thomas B. |
author_facet | Bennuru, Sasisekhar Cotton, James A. Ribeiro, Jose M. C. Grote, Alexandra Harsha, Bhavana Holroyd, Nancy Mhashilkar, Amruta Molina, Douglas M. Randall, Arlo Z. Shandling, Adam D. Unnasch, Thomas R. Ghedin, Elodie Berriman, Matthew Lustigman, Sara Nutman, Thomas B. |
author_sort | Bennuru, Sasisekhar |
collection | PubMed |
description | Onchocerciasis (river blindness) is a neglected tropical disease that has been successfully targeted by mass drug treatment programs in the Americas and small parts of Africa. Achieving the long-term goal of elimination of onchocerciasis, however, requires additional tools, including drugs, vaccines, and biomarkers of infection. Here, we describe the transcriptome and proteome profiles of the major vector and the human host stages (L1, L2, L3, molting L3, L4, adult male, and adult female) of Onchocerca volvulus along with the proteome of each parasitic stage and of its Wolbachia endosymbiont (wOv). In so doing, we have identified stage-specific pathways important to the parasite’s adaptation to its human host during its early development. Further, we generated a protein array that, when screened with well-characterized human samples, identified novel diagnostic biomarkers of O. volvulus infection and new potential vaccine candidates. This immunomic approach not only demonstrates the power of this postgenomic discovery platform but also provides additional tools for onchocerciasis control programs. |
format | Online Article Text |
id | pubmed-5137501 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | American Society for Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-51375012016-12-08 Stage-Specific Transcriptome and Proteome Analyses of the Filarial Parasite Onchocerca volvulus and Its Wolbachia Endosymbiont Bennuru, Sasisekhar Cotton, James A. Ribeiro, Jose M. C. Grote, Alexandra Harsha, Bhavana Holroyd, Nancy Mhashilkar, Amruta Molina, Douglas M. Randall, Arlo Z. Shandling, Adam D. Unnasch, Thomas R. Ghedin, Elodie Berriman, Matthew Lustigman, Sara Nutman, Thomas B. mBio Research Article Onchocerciasis (river blindness) is a neglected tropical disease that has been successfully targeted by mass drug treatment programs in the Americas and small parts of Africa. Achieving the long-term goal of elimination of onchocerciasis, however, requires additional tools, including drugs, vaccines, and biomarkers of infection. Here, we describe the transcriptome and proteome profiles of the major vector and the human host stages (L1, L2, L3, molting L3, L4, adult male, and adult female) of Onchocerca volvulus along with the proteome of each parasitic stage and of its Wolbachia endosymbiont (wOv). In so doing, we have identified stage-specific pathways important to the parasite’s adaptation to its human host during its early development. Further, we generated a protein array that, when screened with well-characterized human samples, identified novel diagnostic biomarkers of O. volvulus infection and new potential vaccine candidates. This immunomic approach not only demonstrates the power of this postgenomic discovery platform but also provides additional tools for onchocerciasis control programs. American Society for Microbiology 2016-11-23 /pmc/articles/PMC5137501/ /pubmed/27881553 http://dx.doi.org/10.1128/mBio.02028-16 Text en Copyright © 2016 Bennuru et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (http://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Article Bennuru, Sasisekhar Cotton, James A. Ribeiro, Jose M. C. Grote, Alexandra Harsha, Bhavana Holroyd, Nancy Mhashilkar, Amruta Molina, Douglas M. Randall, Arlo Z. Shandling, Adam D. Unnasch, Thomas R. Ghedin, Elodie Berriman, Matthew Lustigman, Sara Nutman, Thomas B. Stage-Specific Transcriptome and Proteome Analyses of the Filarial Parasite Onchocerca volvulus and Its Wolbachia Endosymbiont |
title | Stage-Specific Transcriptome and Proteome Analyses of the Filarial Parasite Onchocerca volvulus and Its Wolbachia Endosymbiont |
title_full | Stage-Specific Transcriptome and Proteome Analyses of the Filarial Parasite Onchocerca volvulus and Its Wolbachia Endosymbiont |
title_fullStr | Stage-Specific Transcriptome and Proteome Analyses of the Filarial Parasite Onchocerca volvulus and Its Wolbachia Endosymbiont |
title_full_unstemmed | Stage-Specific Transcriptome and Proteome Analyses of the Filarial Parasite Onchocerca volvulus and Its Wolbachia Endosymbiont |
title_short | Stage-Specific Transcriptome and Proteome Analyses of the Filarial Parasite Onchocerca volvulus and Its Wolbachia Endosymbiont |
title_sort | stage-specific transcriptome and proteome analyses of the filarial parasite onchocerca volvulus and its wolbachia endosymbiont |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5137501/ https://www.ncbi.nlm.nih.gov/pubmed/27881553 http://dx.doi.org/10.1128/mBio.02028-16 |
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