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A first approach to evaluate the cell dose in highly porous scaffolds by using a nondestructive metabolic method
BACKGROUND: In cell-based therapies, in vitro studies on biomimetic cell–scaffold constructs can facilitate the determination of the cell dose, a key factor in guaranteeing the effectiveness of the treatment. However, highly porous scaffolds do not allow a nondestructive evaluation of the cell numbe...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Future Science Ltd
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5137907/ https://www.ncbi.nlm.nih.gov/pubmed/28031911 http://dx.doi.org/10.4155/fso.15.58 |
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author | Divieto, Carla Sassi, Maria Paola |
author_facet | Divieto, Carla Sassi, Maria Paola |
author_sort | Divieto, Carla |
collection | PubMed |
description | BACKGROUND: In cell-based therapies, in vitro studies on biomimetic cell–scaffold constructs can facilitate the determination of the cell dose, a key factor in guaranteeing the effectiveness of the treatment. However, highly porous scaffolds do not allow a nondestructive evaluation of the cell number. Our objective was to develop a nondestructive method for human mesenchymal stem cells dose evaluation in a highly porous scaffold for bone regeneration. MATERIALS & MEASUREMENT METHOD: Proliferation trend of human mesenchymal stem cells on Biocoral(®) scaffolds was measured by a resazurin-based assay here optimized for 3D cultures. The method allows to noninvasively follow the cell proliferation on biocorals over 3 weeks with very high reproducibility. CONCLUSION: This reliable method could be a powerful tool in cell-based therapies for cell dose determination. |
format | Online Article Text |
id | pubmed-5137907 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Future Science Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-51379072016-12-28 A first approach to evaluate the cell dose in highly porous scaffolds by using a nondestructive metabolic method Divieto, Carla Sassi, Maria Paola Future Sci OA Research Article BACKGROUND: In cell-based therapies, in vitro studies on biomimetic cell–scaffold constructs can facilitate the determination of the cell dose, a key factor in guaranteeing the effectiveness of the treatment. However, highly porous scaffolds do not allow a nondestructive evaluation of the cell number. Our objective was to develop a nondestructive method for human mesenchymal stem cells dose evaluation in a highly porous scaffold for bone regeneration. MATERIALS & MEASUREMENT METHOD: Proliferation trend of human mesenchymal stem cells on Biocoral(®) scaffolds was measured by a resazurin-based assay here optimized for 3D cultures. The method allows to noninvasively follow the cell proliferation on biocorals over 3 weeks with very high reproducibility. CONCLUSION: This reliable method could be a powerful tool in cell-based therapies for cell dose determination. Future Science Ltd 2015-11-01 /pmc/articles/PMC5137907/ /pubmed/28031911 http://dx.doi.org/10.4155/fso.15.58 Text en © Divieto & Sassi This work is licensed under a Creative Commons Attribution 4.0 License (http://creativecommons.org/licenses/by/4.0/) |
spellingShingle | Research Article Divieto, Carla Sassi, Maria Paola A first approach to evaluate the cell dose in highly porous scaffolds by using a nondestructive metabolic method |
title | A first approach to evaluate the cell dose in highly porous scaffolds by using a nondestructive metabolic method |
title_full | A first approach to evaluate the cell dose in highly porous scaffolds by using a nondestructive metabolic method |
title_fullStr | A first approach to evaluate the cell dose in highly porous scaffolds by using a nondestructive metabolic method |
title_full_unstemmed | A first approach to evaluate the cell dose in highly porous scaffolds by using a nondestructive metabolic method |
title_short | A first approach to evaluate the cell dose in highly porous scaffolds by using a nondestructive metabolic method |
title_sort | first approach to evaluate the cell dose in highly porous scaffolds by using a nondestructive metabolic method |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5137907/ https://www.ncbi.nlm.nih.gov/pubmed/28031911 http://dx.doi.org/10.4155/fso.15.58 |
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