Development and validation of a clinical HPLC method for the quantification of hydroxychloroquine and its metabolites in whole blood

BACKGROUND: Therapeutic drug monitoring for hydroxychloroquine (HCQ) has been suggested to assess nonadherence and optimize treatment efficacy in systemic lupus erythematosus patients. MATERIALS & METHODS: After protein precipitation, HCQ and its metabolites, desethylhydroxychloroquine and deset...

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Detalles Bibliográficos
Autores principales: Qu, Ying, Noe, Gaelle, Breaud, Autumn R, Vidal, Michel, Clarke, William A, Zahr, Noel, Dervieux, Thierry, Costedoat-Chalumeau, Nathalie, Blanchet, Benoit
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Future Science Ltd 2015
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5137978/
https://www.ncbi.nlm.nih.gov/pubmed/28031899
http://dx.doi.org/10.4155/fso.15.24
Descripción
Sumario:BACKGROUND: Therapeutic drug monitoring for hydroxychloroquine (HCQ) has been suggested to assess nonadherence and optimize treatment efficacy in systemic lupus erythematosus patients. MATERIALS & METHODS: After protein precipitation, HCQ and its metabolites, desethylhydroxychloroquine and desethylchloroquine were separated on a phenyl column and monitored by fluorescence detection. The method was linear from 50 to 4000 ng/ml for HCQ. The intra-day and inter-day precision of HCQ, desethylhydroxychloroquine and desethylchloroquine ranged from 4.3 to 10.3%. LLOQ was 50 ng/ml for HCQ. CONCLUSION: The method is very practical and was applied to routinely monitor the steady state whole blood exposure of HCQ and its metabolites in systemic lupus erythematosus patients. It well correlated with our LC–MS/MS and another HPLC method.

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